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Citations for SouthernBiotech :
551 - 600 of 699 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with neoepitope-targeting mAbs and subsequently detected with a mouse anti-human IgG Fc-PE secondary antibody (SouthernBiotech 9040-09). Detection of the rabbit pAb cocktail was conducted with a goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were mycoplasma free as determined PCR assay (Southern Biotech).
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bioRxiv - Biophysics 2020Quote: ... Cells were mounted onto glass microscope slides with Fluoromount-G (Southern Biotech) containing DAPI (2.5 μg/mL) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then mounted onto slides with Fluoromount with DAPI (Southern Biotech). Micrograph images were obtained using a DeltaVision microscope (Applied Precision ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Immunology 2022Quote: ... cells were mounted on glass slides with Fluoromount-G (SouthernBiotech; 0100-01) and dried overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01, and anti-human IgG, SouthernBiotech, Cat # 2042-01) were conjugated on to selected peaks of Streptavidin coated 4um polystyrene capture beads ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Neuroscience 2022Quote: ... Staining was performed using anti-PrP antibody (clone ICSM18; D-Gen Ltd) followed by detection with alkaline-phosphatase-linked anti-IgG1 antiserum (Southern Biotech; 1070-04). Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane was washed five times in PBS-T before it was incubated with the secondary antibody goat α-rat IgG (H&L)-HRP (1:10,000, Southern Biotech #3050-05) or goat α-mouse IgG (H&L)-HRP (1:10,000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The secondary antibodies were goat anti-rabbit (#4010-05, 1:5000) and anti-mouse (#1030-05, 1:5000, both from Southern Biotech, Birmingham AL) coupled to horseradish peroxidase ...
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bioRxiv - Immunology 2024Quote: ... 100 μl per well of 1 μg/l alkaline phosphatase (AP)-conjugated goat anti-human antibodies (Goat Anti-Human kappa-AP, SouthernBiotech, ref 2060-04) was incubated in PBS with 0.05% Tween® 20 for 2 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... coated overnight at 4°C (100 μl per well) with 2 μg/ml Igκ antibodies (Goat Anti-Human kappa-UNLB, SouthernBiotech, ref 2060-01) in PBS ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with indicated primary antibodies overnight at 4 °C and with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (SouthernBiotech, Birmingham, AL, USA) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 1 hour at room temperature in TBSX with 1:1000 donkey fluorescent secondary antibody and mounted on slides using Fluoromount-G (Southern Biotech 0100-01) before coverslipping ...
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bioRxiv - Immunology 2024Quote: ... Blocking and primary sample incubations were one hour at 37°C and secondary antibody incubations with a 1:6,000 dilution of mouse anti-monkey HRP conjugate (clone SB108a, Southern Biotech, cat. 4700-05) were 45 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed in TBS-T and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human Ig Fc antibody (Southern Biotech, 2047-050) at a dilution of 1:4000 in TBS-TMS for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: Fifty microliters of appropriately diluted serum samples were added to 96-well plates precoated with anti-mouse Igκ and Igλ antibodies (2 mg/ml; Southern Biotech, Birmingham, AL) in 0.1 M carbonate buffer (pH 9.0) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to nitrocellulose or PVDF membrane and probed with rat anti-FLAG (clone 9H1, produced in-house; 1:1000) primary antibody and anti-rat HRP (Southern Biotech; 1:5000) secondary.
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bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly washed in PBS prior to mounting in Fluoromount-G (SouthernBiotech) on glass slides.
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bioRxiv - Developmental Biology 2021Quote: ... and cells were mounted in Fluoromount G (Southern Biotech cat. no. 0100-01). Images were acquired using a Zeiss LSM780 point scanning confocal microscope with a 20X objective.
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bioRxiv - Immunology 2022Quote: ... Cells were mounted using water-based mowiol mounting media (Southern Biotech, Birmingham, AL) to preserve fluorescence ...
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bioRxiv - Immunology 2022Quote: ... Cells were mounted using water-based mowiol mounting media (Southern Biotech, Birmingham, AL) to preserve fluorescence ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted 1:200 at 4°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with FITC-conjugated goat-anti-Rhesus polyclonal antisera (Southern Biotech) to detect binding of the plasma Ab (pAb) ...
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bioRxiv - Molecular Biology 2023Quote: ... coverslips with cells were then mounted in Fluoromount-G® mounting medium (SouthernBiotech) on microscope slides ...
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bioRxiv - Microbiology 2023Quote: ... All cell cultures were verified by PCR to be Mycoplasma-free (Southern Biotech).
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bioRxiv - Bioengineering 2023Quote: ... or without cell type specificity (rabbit anti-human IgG, 6145-01, Southern Biotech) was dissolved in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The stained sections or cells were mounted onto slides with Fluoromount-G (SouthernBiotech) or ProlongTM Glass Antifade Mountant (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were mounted to glass slides using Fluoromeount G DAPI (SouthernBiotech; 0100-20) and imaged on a Zeiss 800 CLSM with Airyscan at the UNMC Advanced Microscopy Core Facility.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed and embedded on glass slides with DAPI Fluoromount-G (SouthernBiotech). Mean nuclear signal is mean gray value calculated with image analysis using FIJI.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and mounted in Fluoromount (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 1:200 of anti-monkey IgG-PE (Southern Biotech) for 25 minutes at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated with Goat anti-mouse IgG2b-AF555 (1091-32, SouthernBiotech) (1:400 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were mounted on slides using Fluoromount mounting medium (Southern Biotech, OB100-01). Images were acquired using an EVOS M5000 Microscope (Invitrogen ...