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Citations for SouthernBiotech :
601 - 650 of 699 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with Annexin V binding buffer (Cat#: 10045-01, SouthernBiotech) then resuspended in 200 µL of binding buffer and stained with 5 µL of 7-AAD (Cat# ...
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bioRxiv - Pathology 2024Quote: ... cells were incubated in DAPI (1 µg/ml, Southern Biotech, Cat # 0100-20) for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed before the addition of goat anti-mouse IgG secondary antibody conjugated with alkaline phosphatase (AP) (Southern Biotech, Birmingham, AL, USA) for 2 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in the secondary antibody at room temperature and washed with TBS prior to slide-mounting with DAPI Fluoromount-G® mounting medium (SouthernBiotech, catalog #0100-20). Primary antibodies included rabbit anti-DsRed (Takara Bio ...
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bioRxiv - Biophysics 2021Quote: ... Cell lines were tested for mycoplasma contamination via the Mycoplasma Detection Kit (Southern Biotech). Transient transfections were performed with Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with goat anti-human IgG Fc-HRP (Southern Biotech, 2014-05) diluted at 1:1000 in Perm Wash for 1.5 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were mounted with DAPI Fluoromount-G media with DAPI nuclear stain (Southern Biotech). Slides were viewed with Olympus microscopy.
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bioRxiv - Neuroscience 2021Quote: ... We then incubated cells with goat-anti-rabbit-APC (Southern Biotech, Cat# 4050-11S), mouse anti-human monoclonal antibodies against p75/NGFR (Becton-Dickinson ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained with DAPI and mounted with Fluoromount-G mounting medium (SouthernBiotech). For β-gal staining ...
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bioRxiv - Neuroscience 2022Quote: ... cells were mounted with DAPI Fluoromount-G® Mounting Media (Southern Biotech, 0100-20). All images were acquired using a confocal laser scanning microscope (LSM 800 with Airyscan ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections or cells were counterstained with DAPI and mounted with Fluoromount-G (Southern Biotech) before being visualized using a Nikon Eclipse Ti microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Stained cells were cover-slipped in DAPI Fluoromount G mounting medium (SouthernBiotech, 0100-20), allowed to dry ...
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bioRxiv - Biochemistry 2023Quote: ... in PBS for 5 min and mounted the cells with Fluoromount-G (Southern Biotech). For negative control ...
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bioRxiv - Immunology 2020Quote: ... or F(ab)2 polyclonal goat anti-human IgM (Jackson Immuno Research) as capture antibody and detection with HRP-conjugated mouse μ-specific anti-human IgM (Southern Biotech, clone SA-DA4).
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... No mycoplasma contamination was detected in this cell line throughout the experiments (SouthernBiotech #13100-01). XPA expression plasmids contain full-length human XPA (NM_000380 ...
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bioRxiv - Immunology 2021Quote: ... As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) and anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed then coincubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted to 1:200 for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were mounted on a glass slide with Fluoromount-G mounting media (Southern Biotech). Images were acquired using confocal LSM780 (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech). For intracellular staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were stained using mouse anti-chicken mABs CD3-PE (clone CT-3; Southern Biotech) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
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bioRxiv - Microbiology 2024Quote: ... cells were labeled with 2 μg/ml PE-conjugated goat anti-human IgG (Southern Biotech) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and incubated for 5 min in ice with streptavidin (60 μg/ml, SouthernBiotech) for cross-linking and incubated at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fluoromount-G used to mount cells for imaging was obtained from Southern Biotech (Cat# 0100-01). AZD1152 (AURKB inhibitor Cat# SML0268 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with PBS three times followed by treatment with DAPI Fluoromount-G (Southern Biotech). Cells were imaged with a Leica SP8 3×STED superresolution microscope.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with PBS and mounted on slides with Fluoromount G (SouthernBiotech #0100-01) for imaging and quantification using the CellInsight CX7 LED Pro High-Content Screening Platform (ThermoFisher Scientific HCSDCX7LEDPRO).
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bioRxiv - Genomics 2023Quote: ... Cells were checked for mycoplasma contamination using the Mycoplasma detection kit (Southern Biotech, catalog #13100-01) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with goat anti-mouse IgG (H + L)–FITC (1:5000 dilution, Southern Biotech) and DAPI (500ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were mounted on microscopy slides in DAPI Fluoromount-G mounting medium (0100-20, Southern Biotech), which stains the nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed again 3x with PBS before allowing to dry and mounting with Fluoromount-G (SouthernBioTech).
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bioRxiv - Immunology 2022Quote: ... Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L) and HRP-streptavidin (Southern Biotech). The reactions were developed with o-Phenylenediamine di-hydrochloridec (OPD ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected and stained on ice with Alexa-fluor 488-coupled anti-IgA Ab (Southern Biotech) and 7AAD (Roche ...
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were then activated with 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) or left resting ...
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bioRxiv - Molecular Biology 2024Quote: ... Glass slides with the cells were washed with PBS and mounted with DAPI Fluoromount-G (SouthernBiotech, USA). LSM 510 META inverted confocal microscope at a 40× magnification (Carl Zeiss Micro Imaging ...
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bioRxiv - Microbiology 2024Quote: ... Cells were routinely confirmed to be mycoplasma free by conducting PCRs using a mycoplasma detection kit (SouthernBiotech).
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then washed and mounted on the slide glass using fluromount-G® (SouthernBiotech, 011-01).
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times with 1x PBST and mounted with DAPI Fluoromount-G® (SouthernBiotech, AL).
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 8x in 1x PBS then mounted in a DAPI-containing mounting medium (Southern Biotech). Slides were sealed with nail varnish then stored at 4 °C in the dark until required ...