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Citations for Millipore Sigma :
151 - 200 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: DNA primers (Sigma) were radiolabeled at the 5’-end with 32P using the manufacturer recommended protocol for T4 PNK (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Primers (Sigma-Aldrich) for qRT-PCR were intron-spanning to avoid amplification of non-digested genomic DNA fragments and were designed by online Universal ProbeLibrary Assay Design Center (Roche) ...
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bioRxiv - Zoology 2021Quote: ... Primers (Sigma Aldrich) used to amplify D2/D3LSU segment of all three species ...
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bioRxiv - Cell Biology 2022Quote: ... mutagenic primers (Sigma) were created for the desired mutations ...
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bioRxiv - Cell Biology 2019Quote: ... mutagenic primers (Sigma) were created for the desired mutations ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Sigma-Aldrich) were as follows ...
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bioRxiv - Plant Biology 2021Quote: ... and primers (Sigma) listed in Supplemental Table S1 ...
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bioRxiv - Genomics 2022Quote: ... Oligonucleotide primers (Sigma) are listed in Table S9 ...
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bioRxiv - Cell Biology 2023Quote: ... Primer sequences (Sigma) are listed in Table 1 ...
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bioRxiv - Genomics 2023Quote: ... qPCR primers (Sigma) were designed to amplify 75-200bp amplicons and were checked for specificity using Primer-BLAST (ncbi.nlm.nih.gov) ...
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bioRxiv - Cell Biology 2023Quote: ... Primers (Sigma Aldrich) and probes (MWG ...
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bioRxiv - Developmental Biology 2023Quote: Primers (Sigma-Aldrich) were designed to amplify exon–exon junctions using PrimerBlast (NIH ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed on 100 ng of cDNA in triplicate using SYBR Green Jumpstart Taq readymix (Sigma). Primers RMC379/380 (16S internal) ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The cDNA samples generated with RT PCR were then used for performing qPCR using SYBR Green I (Sigma) and Platinum Taq polymerase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Immunology 2022Quote: ... mature miR-31 was quantified using MystiCq® microRNA qPCR Assay (Sigma, MIRRM00-100RXN) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a loaded MLNPs or empty MLNPs were lysed using 1% TritonX-100 (Sigma) and diluted in a working solution ...
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bioRxiv - Cell Biology 2020Quote: ... The primers used for qPCR were Kicq predesigned primers (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... All primers used for qPCR were Kicq pre-designed primers (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... predesigned primers KiCqStart® SYBR® Green Primers (Sigma-Aldrich, # KSPQ12012) were used for gene expression analysis ...
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bioRxiv - Developmental Biology 2022Quote: DNA fragments were released by restriction from existing plasmids or amplified by PCR using primers synthesized by Sigma-Aldrich or Eurofins ...
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bioRxiv - Cell Biology 2020Quote: ... Mutagenic primers were used to amplify pFA6a-mto2S338N-C-KanMX6 using the Expand High Fidelity PCR system (Millipore Sigma). The PCR product was digested with DpnI and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Plant Biology 2022Quote: ... were also amplified by PCR using the primers listed in Supplemental Table 2 and cloned into pGEX4T-1 (Novagen) using BamHI/EcoRI sites to N-terminal glutathione S-transferase (GST ...
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bioRxiv - Molecular Biology 2023Quote: ... We verified candidate plasmids using colony PCR using primers listed in Supplemental Table 2 and REDTaq ReadyMix (Sigma-Aldrich). All plasmids were miniprepped (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail set III (Cat No. 539134-1 set, 1:100, Merck Millipore, Rahway, NJ). The lysate was centrifuged at 16,000 g at 4 LJ for 10 minutes to remove insoluble samples ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.2% NP40) supplemented with 1% protease inhibitor cocktail set III and 1% phosphatase inhibitor cocktail set II (Millipore). Following clarification by centrifiguation ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail set III (Merck Millipore), 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail set III (Merck Millipore), 1 mM PMSF) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease Inhibitor Cocktail Set III (EMD Millipore)) on ice for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Protease Inhibitor Cocktail Set III (Merck Millipore) and SUPERase• In RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... protease (Protease Inhibitor Cocktail Set III, Sigma)] and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... and phosphatase inhibitor set II (Sigma, 524625). Protein extracts were quantified using Pierce BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... to sort each well containing 80 nuclei per well into new four 96-well plates (in total of 384 wells) containing 8 μL primer dilution buffers (15 pmol primer i7, 15 pmol primer i5, 200 ng BSA (Sigma), 0.027% SDS ...
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bioRxiv - Neuroscience 2019Quote: ... RT-PCR was performed in a Biorad CFX384 using KiCqStart SYBR Green Ready Mix (Millipore-Sigma, St. Louis, MO). RNA expression was normalized to the housekeeping gene GADPH and quantified using the ΔΔCt method ...
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bioRxiv - Neuroscience 2019Quote: ... RT-PCR was performed in a Biorad CFX384 using KiCqStart SYBR Green Ready Mix (Millipore-Sigma, St. Louis, MO). RNA expression was normalized to the housekeeping gene GADPH and quantified using the ΔΔCt method ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600) with a LightCycler 480 apparatus (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... The primers used for the genes were KiCqStart SYBR green primers (Sigma) and the primers for miRNAs are listed in Table-2.
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bioRxiv - Cancer Biology 2019Quote: ... 5′ to 3′ Primer sequences: Custom primers were obtained from Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2020Quote: ... Primer sequences used were predesigned KiCqStart SYBR® Green primers (Sigma-Aldrich) and are included in Appendix Table S13 ...
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bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set I #524624 and Phosphatase Inhibitor Cocktail Set II #524625; Millipore Sigma-Aldrich), was added directly on the plated cells and lysed for 15 min on ice ...
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bioRxiv - Systems Biology 2024Quote: ... the negative control set as 0.1% DMSO and the positive control set as 100 µM etoposide (Sigma Aldrich, Germany), and a media blank column (plated per column) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequences of primers (Sigma) used for genotyping are listed in Table S1 in Supplementary Material ...
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bioRxiv - Genetics 2021Quote: Primers (Sigma, BLOT solutions), Restriction enzymes ...
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bioRxiv - Synthetic Biology 2021Quote: ... and TruSeq primers (Sigma). Libraries were gel purified (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... and TruSeq primers (Sigma). Libraries were gel purified (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... using primers (Sigma-Aldrich) and amplification program described by McEvoy et al ...
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bioRxiv - Cell Biology 2021Quote: ... We designed primers (Sigma) as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... Lyophilised primers (Sigma Aldrich) were designed using exon spanning sequences in primer3 ...
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bioRxiv - Neuroscience 2021Quote: ... oligonucleotide primers (Sigma Aldrich) specific to target genes and SYBR Green (KAPA Syber1 FAST PCR Master Mix (2X ...
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bioRxiv - Immunology 2021Quote: ... NLRP3 primer pair (Sigma): forward 5’ -TCAGCACTAATCAGAATCTCACGCACCTTT -3’ and reverse 5’ -CCAGGTCATTGTTGCCCAGGCTC -3’ ...