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Citations for Millipore Sigma :
51 - 100 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... SybrGreen Quantitative RT-PCR kit (Sigma Aldrich, Germany) was used with Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The VH and VL sequences of B6 were recovered using the mouse iG primer set (Millipore) using the protocol outlined in (Siegel ...
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bioRxiv - Microbiology 2020Quote: ... KiCqStart SYBR green primers for qRT-PCR (Sigma-Aldrich) were specific for MCM2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR primers were synthesized by Sigma Aldrich (S2 Table), longer DNA fragments and genes were ordered from GeneScript (S3 Table) ...
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bioRxiv - Microbiology 2020Quote: ... PCR primers and oligos were purchased from Sigma-Aldrich. Restriction enzymes were purchased from New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046). DMSO solution was used as a control ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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bioRxiv - Genetics 2022Quote: ... or miR-27b-3p mimics (Sigma) for 48 h ...
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bioRxiv - Biochemistry 2019Quote: Genotyping of founders (F0) was done using genomic PCR with the primers following primers provided by Sigma, forward 5’-CGCGATGACAGTTTCCAGTA-3’ reverse 5’-CAAACAAAAACCCACTGAGGA-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... DNA oligonucleotides (qRT-PCR primers) were obtained from Sigma-Aldrich.
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bioRxiv - Systems Biology 2020Quote: ... Linear products were created by PCR with primers from Sigma Life Science and Q5 Polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... Linear products were created through PCR with primers from Sigma Life Science and Q5 Polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were then amplified by PCR with TruSeq primers (Sigma) and Phusion polymerase (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Primers used for genotyping PCR were synthesized by Sigma-Aldrich. Oligonucleotide sequences are given in Table 2.
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was carried out as previously described [20] using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes (see Supp Table 1 for primer list) ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using Kapa SYBR Fast (Sigma). SREBP genes primer sequences are listed in Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2020Quote: RT-PCR: 106 cells were lysed in TRI reagent (Sigma) and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research) ...
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bioRxiv - Microbiology 2020Quote: ... Samples for quantitative PCR and quantitative RT-PCR were homogenized in TRiZOL reagent (Sigma Aldrich) and further processed for nucleic acid extractions using manufacturer’s protocols.
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bioRxiv - Microbiology 2021Quote: ... For gene expression analysis one-step qRT-PCR (SYBR Green Quantitative RT-PCR kit, Sigma) was carried out ...
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bioRxiv - Neuroscience 2023Quote: ... mice were intraperitoneally injected with 40 mg/kg RU-486 (also referred as mifepristone; M8046; Sigma-Aldrich). Metyrapone and RU-486 were freshly dissolved in DMSO and then diluted 1:100 in pure corn oil ...
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bioRxiv - Neuroscience 2021Quote: ... PCR mix was prepared using gene-specific primers (0.6 μM, Sigma, primer sequences in the Appendix Table S1 and S2 ...
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bioRxiv - Microbiology 2020Quote: ... Primers for PCR amplification of fragments were ordered from Sigma-Aldrich. All primers and oligonucleotides used for cloning are listed in the table below.
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bioRxiv - Molecular Biology 2023Quote: ... Primers utilized to produce PCR fragments were purchased from Sigma-Aldrich.
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bioRxiv - Microbiology 2023Quote: All PCR primers used in this study were synthesized by Sigma and are indicated Supplementary Table S1.
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bioRxiv - Cancer Biology 2023Quote: Custom primers for real-time PCR were purchased from Sigma-Aldrich, Germany (GAPDH ...
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bioRxiv - Developmental Biology 2023Quote: ... TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 μM, Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout ...
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bioRxiv - Developmental Biology 2023Quote: ... TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 μM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
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bioRxiv - Genomics 2019Quote: ... was used along with 0.36 μl of 50 μM custom-designed SYNE2 short and long isoform primer and 0.5 μl of 10 μM probe set (Table 1, obtained from Sigma-Aldrich) and 1 μl of the primer limited cyclophilin A (PPIA ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). ELISAs were performed as per the manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Overlapping PCR primers encoding the gRNA sequences were ordered from Sigma-Aldrich and Eurofins Genomics ...
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: Colonies that produced bands of the expected size with both primer sets were inoculated into 1 ml of LB containing 1% arabinose (Sigma-Aldrich) for 1 hour at 30°C to induce I-Sce expression to linearize the inserted KanSacB cassette ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Cancer Biology 2023Quote: miR-6883 mimic was purchased from Sigma-Aldrich (HMI2616). This double-stranded RNA molecule mimics endogenous mature miRNA when introduced into cells.
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Cell Biology 2022Quote: Individual cells were sorted into 96-well plates containing 4μL of lysis buffer and index RT primers (0,2% Triton (Sigma Aldrich), 0,4 U/μL RNaseOUT (Thermofisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence was verified by colony PCR using standard primers (Sigma-Aldrich, Taufkirchen, Germany) and Taq polymerase (In house ...
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bioRxiv - Genetics 2023Quote: ... specific primers were designed and PCR was performed using KOD DNA polymerase (Sigma Aldrich). The amplification of DNA fragments was done following manufacturer’s instructions into a Bioer GeneExplorer thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.