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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Lipid droplets staining was performed by immersing cells in 0.1 μg mL−1 Nile red (Sigma) for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... The lysate was then mixed with 2 ml of 50% Iodixanol (Sigma-Aldrich D1556) to generate a nuclei suspension with 20% Iodixanol ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle lysates were further digested with 2 IU/ml of Dispase II (Sigma Aldrich) diluted in minimum amount of PBS for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... remaining lysate was equally divided and incubated with 2 µg Pan-Ago (Millipore; 2A8) or IgG (Cell Signaling 5415 ...
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bioRxiv - Genomics 2023Quote: ... The lysate was then mixed with 2 mL of 50% Iodixanol (Sigma-Aldrich D1556) to generate a nuclei suspension with 20% Iodixanol ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells infected with JR-FL without Env (No Env virus) were treated with 100 mM 2-deoxy-glucose (2-DG) (Sigma-Aldrich, St. Louis, MO, USA) 2 h prior to image acquisition.
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Biochemistry 2019Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Sigma) and oligo dT (Sigma ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Biophysics 2021Quote: ... The cells were then washed twice with PBS and incubated with 5 μM Nile Red (Sigma, N3013) and 1 μg/mL Hoechst 33342 (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... rinse 3 times in 1XPBS without detergent and stained with Nile red (Cat. No. 72485, Sigma-Aldrich) or Bodipy™ 493/503 (Cat ...
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bioRxiv - Microbiology 2019Quote: ... Lipids were stained with Nile red and DNA was stained with Hoechst 33342 (Sigma-Aldrich, Munich, Germany). Therefore 10 µl of the cell suspensions were centrifuged for 5 min at 8,000 x g and the pellet was resuspended in 500 µl PBS containing 100 ng/ml Hoechst 33342 and 250 ng/ml Nile red ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was stained with 1:2000 dilution of 0.5 mg/ml Nile Red (Sigma Aldrich, N3013) in PBS for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... PHAs were stained in the biological samples with Nile red (CAS n. 7385-67-3, Sigma Aldricht), as in (34) ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Genomics 2019Quote: ... The lysate was transferred to a pre-chilled 2 ml dounce homogenizer (Sigma-Aldrich D8938) and dounced using loose and tight pestles for 20 times each ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... hTert-BJ1 or U937 were infected with 2 ml of fresh virus in presence of 8µg/ml Protamine (Millipore Sigma). To generate transduced MDDCs ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Microbiology 2019Quote: ... Anti-influenza A virus NP antibodies (Millipore), Anti-TNK2 (A12 ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... grids were incubated with secondary antibody Alexa 488 and in the last 5 minutes Nile red (Sigma, 72485) and Hoechst 33342 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were again washed twice with PBS and then stained with 150 nM Nile Red (Sigma-Aldrich, 72485) in PBS for 15 minutes at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The remainder of the lysate was incubated with 2 µg of anti-FLAG M2 (Sigma, F1804), SUZ12 (CST ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg of clarified lysate was incubated with 40 μL of rinsed streptavidin beads (Sigma 11641786001) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins in the lysate were reduced with 20mM Tris(2-carboxyethyl)phosphine (TCEP) (Sigma-Aldrich) and alkylated with 55mM 2-Chloroacetamide (CAA ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were prepared by resuspending pellets in MilliQ H2O and 2×Laemmli sample buffer (Sigma) and heating at 95°C for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2 ml bed volume Anti-FLAG M2 Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for two hours prior to application to a gravity flow column ...
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bioRxiv - Microbiology 2019Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56 °C ...
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bioRxiv - Microbiology 2021Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56°C ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Plant Biology 2021Quote: ... sorokiniana cells from appropriate treatment were first normalized to 0.2 O.D and then 10 μL of a 100 μg/ml stock of nile red (Sigma Aldrich) in DMSO was added to 100 μL of the culture ...
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bioRxiv - Plant Biology 2019Quote: Lipid droplets were stained with addition of Nile red stock (0.1 mg per mL in acetone) (Sigma-Aldrich, USA) at a final concentration of 1 μg per mL followed by a 20 min incubation in the dark at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 1ml of culture was incubated for 5 minutes at 37°C with 5 μg/ml of nile red (Sigma) to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...