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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were treated with 2 mM 1,10-phenanthroline (Sigma), cross-linked with 0.5 mM DSP (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... 2.87 µL of a 1mg/mL stock of Nile red (Sigma) was added to each sample and incubated at 30°C shaking for an additional 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... or with Nile Red (1 µg/ml; Sigma 19123 Oakville, ON) and DAPI (2 µg/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Whole mount epidermal preparations were stained with Nile Blue (Sigma-Aldrich) and Oil Red O (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and fluorescent dye nile red (NR) were manufactured by Sigma-Aldrich Co ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Whole mount epidermal preparations were stained with Nile Blue (Sigma-Aldrich) and Oil Red O (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... or 10 μg/mL Nile red (Sigma, St. Louis, Missouri, USA). The culture containing the staining solution was incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: The intracellular lipid bodies were stained with Nile red (Sigma-Aldrich) fluorescence dye following Greenspan ...
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bioRxiv - Neuroscience 2023Quote: Larvae were washed and subsequently stained with Nile Red (Sigma, Israel), which specifically stains neutral lipids ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: ... and then stained with the Nile Red solution (Sigma-Aldrich, Germany) and KI/I2 solution ...
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bioRxiv - Cancer Biology 2023Quote: Lipid accumulation was assessed by Nile Red staining (Sigma-Aldrich N3013). Primary hepatocytes were isolated and seeded onto chambered coverslips (IBIDI ...
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bioRxiv - Developmental Biology 2024Quote: ... Fixed brains were incubated in the Nile Red solution (Sigma Aldrich, 19123 ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Spores were incubated with Nile red for 30 minutes in the dark followed by 2 µl of Calcofluor White (Sigma-Aldrich 18909). Polar tubes were imaged at 63x magnification using an Axio Imager.M2 (Zeiss) ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Cell Biology 2022Quote: ... or with Nile Red (10 μg/ ml; Sigma-Aldrich, St Louis, MO) for 5 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... and incubated for 30 min in a 0.01mM Nile Red Solution (SIGMA) and with 1:100 Alexa Fluor 488 Phalloidin to visualize the cytoskeleton through the binding between Phalloidin and F-actin ...
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bioRxiv - Cell Biology 2023Quote: ... for an hour or with 1 μg/ml Nile Red (Sigma, 72485) for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Lipid storage function was assessed using Nile red staining (Sigma, MO, USA).
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Neuroscience 2022Quote: ... freshly made from a 1mg/ml stock of nile red (72485, Millipore Sigma) dissolved in acetone stock solution ...
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bioRxiv - Neuroscience 2021Quote: ... and subjected to lipid staining with Nile Red N3013 Technical grade (Sigma-Aldrich) as previously described (Martelli et al. ...
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bioRxiv - Genetics 2023Quote: ... and subjected to lipid staining with Nile Red N3013 Technical grade (Sigma-Aldrich) and nucleic acid staining with DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... FAF-BSA (cat. no. A8806) and Nile red were from Sigma-Aldrich (USA). High Pure RNA isolation kits were from Roche (Germany) ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Nile Red (10 μg/ml; Sigma-Aldrich, St Louis, MO) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with Nile Red (10 μg/ml, Sigma-Aldrich, St Louis, MO) for 5 min at room temperature and washed twice with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with a 0.3 mM Nile Red (Sigma Aldrich, ON, Canada) solution for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was filtered through 2-layers of miracloth (Millipore, Cat. 475855) set up in a 50[ml centrifuge tube ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards cells were co-stained with the fluorescent dyes Nile Red (0.5 μg/ml, Sigma) and Hoechst 33342 (1 μg/ml ...
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bioRxiv - Genomics 2022Quote: ... Packing efficiency of the emulsion was analyzed using 3D lightsheet microscopy with Nile Red (Sigma) stained emulsion oil ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixed tissues were stained using solution of 0.005% (1:200) Nile Red 555 (Sigma; 19123) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... C4-2 cell lysates were incubated with antibodies for AR (Sigma, Cat. No.A9853), YAP (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... samples were incubated with benzonase (Sigma Aldrich, 2 μl/500 μl of lysate) for 90 minutes at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.05 MgSO4 per liter of deionized water) agar plates containing Nile red (Sigma-Aldrich, Missouri, USA) at 0.5 mg per liter ...