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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were then treated with RNase A (Sigma) at 37°C 1 hr in Lysis buffer 2 (Lysis buffer 1 with 0.5% Triton X-100 ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were immunoprecipitated using protein G (Sigma-Aldrich) beads coated with unconjugated anti-biotin antibody (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate concentration was estimated via Bradford Assay (Sigma) and a NanoDrop 2000 (Thermofisher).
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bioRxiv - Microbiology 2022Quote: ... Lysate was applied to an equilibrated HisTrap (Sigma) Ni-sepharose column and washed with 10mL wash Buffer A (50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were incubated with Ni-NTA beads (Novagen) for 3 h and washed with BC300 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with TALON resin (Novagen) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein lysates were incubated with Glutathione-Agarose (Sigma) at 4 °C for 2 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cell lysates were harvested in TRI Reagent (Sigma). Total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was treated with 9U/mL benzonase (Sigma) and clarified by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were treated with benzonase (707463, Novagen) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... In brief lysates were alkylated (iodoacetamide, Sigma, # I1149) for 4h at RT ...
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bioRxiv - Immunology 2023Quote: ... Lysates were treated with benzonase (Sigma, E1014- 25KU) and RNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the lysate was digested with DNase/RNase (Sigma) and treated with 2% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5% human platelet lysate (Sigma #SCM152) and 50 μg/ml Primocin (InvivoGen #ant-pm-2) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were treated with Benzonase Nuclease (Sigma-Aldrich) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5% human platelet lysate (Sigma #SCM152), w/o antibiotics and exchanged to the plated cell medium ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was incubated with benzonase (Sigma-Aldrich) at 8°C for 20 min and then applied on a 5 ml MBP-trap column (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Microbiology 2021Quote: ... The virus/antibody mixture was replaced with an overly media containing 1% CMC (Sigma) before incubation for 24 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Biochemistry 2023Quote: ... The well was then washed 5X with 500 µL of HPLC purified water before 200 µL buffer (which consists of 10nM Nile Red (Sigma-Aldrich, 72485-1G in PBS or GLOX buffer). The wells were then sealed either with Nescofilm or TWINSIL before imaging on a custom built TAB/dSTROM super-resolution microscope (80).
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...