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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Developmental Biology 2021Quote: We used an in situ Cell Death Fluorescein Kit (Millipore Sigma, Cat. # 11684795910) to detect cells undergoing apoptosis.
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bioRxiv - Neuroscience 2020Quote: ... Cell death was assessed by TUNEL assay (with TMR, red-dUTP, Sigma, 12156792910) and nuclei were labeled with DAPI stain (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed at 48 hours using propidium iodide (PI) (Sigma, P4864) uptake ...
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bioRxiv - Immunology 2022Quote: ... Tunel staining was performed with the In Situ Cell Death detection kit (Sigma) as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Root cell death was quantified using Evans blue dye (Sigma-Aldrich, Taufkirchen, Germany) to assess S ...
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bioRxiv - Cell Biology 2023Quote: For induction of ferroptotic cell death we used (1S,3R)-RSL3 (Sigma-Aldrich) and Imidazole Ketone Erastin (IKE ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptosis was determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich) per manufacture’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Physiology 2020Quote: ... then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma Aldrich) at ZT6 (time “0 minutes”).
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bioRxiv - Biochemistry 2021Quote: Human recombinant SRPK2 (0.4 ug/reaction; EMD Millipore 14-666; Glu46-end) and SRSF2 (0.8 ug/reaction ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell death was measured by incubating the samples with propidium iodide (PI, Sigma- Aldrich), to a concentration of 1 μg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell death exclusion was always applied by staining with DAPI (Sigma cat. D9542-50MG). Sorting was performed on a BD FACS Aria III or BD Influx (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... cell death was quantitated with 8 μg/ml propidium iodide (Sigma, ref. no. 81845). Unless otherwise indicated ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death was assessed 72 hours after addition of DNR (Sigma, cat no: 30450) or Ara-C (LKT Laboratories ...
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bioRxiv - Pathology 2020Quote: ... and incubated with TUNEL reaction mixture (In Situ Cell Death Detection Kit; Sigma, 11684795910) for 1 hr at 37°C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cell death was assessed using SYTOX™ green nucleic acid stain (S7020, Sigma). 0.3-1 µl of 5 mM stain was added to the sample and images acquired ...
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bioRxiv - Molecular Biology 2024Quote: ... Infection and cell death were measured with a Guava EasyCyte Plus flow cytometer (Millipore). Cell death was measured by 7AAD (7-aminoactinomycin D ...