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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: The human K562 cells(Sigma-Aldrich) were grown at 37 °C in RPMI 1640 medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... human cardiomyocyte AC16 cells (Millipore; SCC109) in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Human bronchial epithelial cells (16HBE14o-, Sigma) were maintained in medium consisting of α-MEM (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence in human cells was conducted using antibodies against mouse □-tubulin (Sigma, 1:1000), mouse CENP-E (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Immunology 2021Quote: ... 1 U/kg human insulin (Sigma) was given i.p ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/mL human fibrinogen (Millipore), and 10 U/mL heparin (Sigma-Aldrich)).
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bioRxiv - Cell Biology 2023Quote: ... 1 ug/ml human insulin (Sigma), 10 nM hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:2000 human anti-CREST (Sigma) and donkey anti-human Dylight550 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1 % human albumin (Sigma). PBMC were thawed and rested overnight in X-Vivo-20 ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Bioengineering 2023Quote: ... Cell death was quantified based on the abundance of lactate dehydrogenase (LDH) between treated and non-treated samples (MAK066, Sigma-Aldrich, USA). Absolute LDH content was determined with a standard curve ...
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bioRxiv - Plant Biology 2023Quote: ... plants were washed three times in ddH2 O before cell death staining in a 2.5 mM Evans blue solution (Sigma-Aldrich, lot #MKCH7958) dissolved in 0.1 M CaCl2 pH 5.6 for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with pLKO.1 shRNA plasmid (Sigma, Mission human genome shRNA library) and packaging plasmids-delta 8.9 ...
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bioRxiv - Immunology 2023Quote: ... Human acute monocytic leukemia cell line (THP-1) was cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...