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Citations for Millipore Sigma :
651 - 700 of 4627 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... to which helicase (as indicated) and biotin (Sigma-Aldrich) were added to 100 µM (acting as a trap for free streptavidin ...
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bioRxiv - Bioengineering 2024Quote: ... BSA-biotin (Cat. #A8549, Sigma-Aldrich, Oakville, Ontario, Canada), Tween 20 (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... wheat germ agglutinin (biotin-WGA, Sigma; 1 μg/mL) and imaged by binding of IRDye-streptavidin (Licor ...
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bioRxiv - Biophysics 2023Quote: Biotin (244.3 Da) was purchased from Sigma-Aldrich (# B4639). Biotinylated fluoresceine isothiocyanate (b-FITC ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: The following lectins (biotin conjugates) were used for far-western blotting at the indicated final concentrations: Concanavalin A (biotin-ConA, Sigma; 1 μg/mL), wheat germ agglutinin (biotin-WGA ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Cell Biology 2020Quote: ... 10 μg/ml d-biotin (Sigma-Aldrich, St Louis, USA), 20 ng/ml recombinant human epidermal growth factor (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Reagents used in this study were Biotin (Sigma-Aldrich, B4501), Rhosin (Calbiochem ...
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bioRxiv - Biochemistry 2022Quote: ... For the reduction-cleavable reagent (Azo Biotin-azide, Sigma-Aldrich) ligated sample ...
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bioRxiv - Microbiology 2019Quote: ... biotin incorporation was revealed using streptavidin conjugated to HRP (Sigma). Primary antibodies used in the western blots were the mouse clone 7D2 against TG2 (#ABIN1109303 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μL of 5 mM biotin picolylazide (900912 Sigma-Aldrich) and 25 μL of click reaction mix (3 parts TBTA 5 mM TBTA in butanol:DMSO (4:1 ...
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bioRxiv - Neuroscience 2021Quote: ... IB4-biotin conjugate (1:200, Sigma, St. Louis, MO, #L2140), rabbit anti-CGRP (1:2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Then the cells were treated with 100 μM Biotin (Sigma) in 0.1% FBS DMEM with or without 1 ng/mL EGF for 12 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of click reagent (Dibenzocyclooctyne biotin conjugate; Sigma #760749) diluted in PBS was added (5 μM final ...
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bioRxiv - Genomics 2022Quote: ... 1% Triton X-100 and 1 mM Biotin (Sigma-Aldrich)
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bioRxiv - Microbiology 2022Quote: ... After incubating with 100 μM biotin (Sigma-Aldrich, cat#B4639) at 37°C for 3 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in media containing 50 μM biotin (Sigma) for 24 h ...
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bioRxiv - Plant Biology 2021Quote: ... digoxigenin-11-dUTP or biotin-16-dUTP (all Sigma-Aldrich) and FISH were performed according to Jenkins and Hasterok (2007 ...
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bioRxiv - Neuroscience 2021Quote: ... WFA biotin conjugate was used (Sigma L1516, dilution 1:1000), followed by incubation with Streptavidin (Alexa Fluor™ 594 conjugate ...
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bioRxiv - Neuroscience 2022Quote: ... Biotin-conjugated Cholera Toxin B subunit (CTB, Sigma-Aldrich, C9972) or AAVrg-CAG-GFP (Addgene viral prep 37825-AAVrg ...