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Citations for Millipore Sigma :
501 - 550 of 4627 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... using Biotin RNA Labeling Mix (Sigma Aldrich). Prior to experiments ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng/mL biotin (#B4639, Sigma-Aldrich), 5 µg/mL insulin (#I1882 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with biotin (Sigma Aldrich). Sections were then probed with anti-B220 (clone RA3-6B2 ...
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bioRxiv - Neuroscience 2022Quote: ... and Biotin (1 μg/mL,Sigma, B4501) were injected intravenously into C57 mice ...
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bioRxiv - Biophysics 2022Quote: ... stored and biotinylated using biotin-NHS (Sigma) as previously described ...
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bioRxiv - Biophysics 2022Quote: ... or biotin with PEG4 linker (Sigma Aldrich); or ∼0.15 mM oligo DNA ...
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bioRxiv - Bioengineering 2022Quote: ... biotin (50 μg.mL−1, Sigma, B4639, USA) and ZellSchield® 1X (ZellSchield® ...
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bioRxiv - Cell Biology 2022Quote: ... or NHS-dPEG®4-biotin (Sigma), the same steps were followed as RMA purification until pelleted filamentous actin was homogenized and sonicated ...
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bioRxiv - Biochemistry 2021Quote: A 2.05 mM biotin (Sigma-Aldrich B4501) stock was prepared in distilled water by brief sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... and biotin RNA labeling mix (Sigma Aldrich) following the manufacturer’s recommendations for 2h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 40 μM D-biotin (Sigma-Aldrich) for the indicated time ...
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bioRxiv - Microbiology 2020Quote: ... and 0.1 g/L biotin (Sigma B4639), was added (1 mL per L of medium) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 ng/mL biotin (B4501; Sigma-Aldrich), 2 mg/ml insulin (I5500 ...
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bioRxiv - Physiology 2022Quote: ... 33 μM d-biotin (Sigma-Aldrich, B4639), 17 μM pantothenate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... or biotin-UTP-labelled probes (Sigma, 11685597910) were performed as described by (Kosman et al. ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of D-biotin (Sigma) at 25 μM final concentration to block any free binding site ...
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bioRxiv - Cell Biology 2020Quote: ... and Biotin (Sigma 29129, dissolved in DMEM). All negative controls were treated with the equivalent volume of solvent ...
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bioRxiv - Biophysics 2021Quote: ... 1 mg ml−1 BSA-biotin (Sigma) was introduced into the flow chamber ...
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bioRxiv - Immunology 2022Quote: ... Biotin-N-hydroxysuccinimide (Sigma-Aldrich, H1759-5mg) was dissolved in DMSO to a concentration of 0.4 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin (500 μM,Sigma-Aldrich; B4501-5G) was added to the media 30 minutes before fixation ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse Cy3 anti-biotin antibody (#C5585 - Sigma) at 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... sorbitol and D-Biotin from Sigma Aldrich. Select agar and Zeocin™ were obtained from Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... 33 nM Biotin (Sigma-Aldrich, B-4639), 17 nM Panthotenate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Click reaction with biotin-azide (Sigma-Aldrich) was performed for 1h at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100μM Azide-PEG3-biotin conjugate (762024 Sigma) for 1h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... azide-PEG3-biotin (0.1 mM; Sigma-Aldrich) and cooper(II)sulfate (2 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase-anti-peroxidase for detecting TAP-tagged proteins (Sigma); anti-FLAG mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Cancer Biology 2022Quote: ... an NTP labeling mixture containing biotin-UTP (10 mM each ATP, CTP, and GTP, 6.5 mM UTP, 3.5 mM biotin-UTP; Sigma, 11685597910) was used for in vitro transcription.
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bioRxiv - Microbiology 2022Quote: ... Biotin labeled RNA probes were generated by adding 1.25 μl of 10 mM biotin-16-UTP (Millipore Sigma 11388908910) to the in vitro transcription reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Step 1: a solution of 200 μg/mL BSA-biotin (biotin conjugated Bovine serum albumin, A8549, Sigma-Aldrich, USA) and 2 μg/mL fibronectin (1918-FN ...
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bioRxiv - Molecular Biology 2023Quote: Proximity ligation assay using antibodies to RADX and an anti-biotin antibody to recognize EdU after conjugation to a biotin-azide were completed according to the manufacturer’s protocol (Sigma) and images obtained and quantified using a Molecular Devices ImageXpress instrument.
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...