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Citations for Millipore Sigma :
601 - 650 of 4627 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST tag (Step 2 ...
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bioRxiv - Cell Biology 2022Quote: ... The release of the RUSH cargos from the ER-hook was induced by removing the streptavidin supplemented media and addition of biotin supplemented media (3 mM of D-biotin, B4501 Sigma-Aldrich) for the indicated times.
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bioRxiv - Cancer Biology 2022Quote: ... click chemistry reactions were performed on pre-permeabilized samples to conjugate biotin to the EdU-labeled DNA by using Biotin Picolyl azide (Sigma Aldrich). Sonication was performed with a Bioruptor Pico sonicator (Diagenode ...
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bioRxiv - Biophysics 2024Quote: ... Polyethylene glycol (PEG)-8000 (catalog no. P2139-500G) and Bovine Serum Albumin-biotin (BSA-biotin) (catalog no. A8549-10MG) were obtained from Sigma-Aldrich. Round and square # 1.5 coverslips (catalog no ...
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bioRxiv - Biophysics 2024Quote: ... We used the docking strand R4* modified with biotin on its 5’ end and immobilized it onto coverslip surface functionalized with BSA-biotin (A8549, Sigma-Aldrich) and then Neutravidin (31000 ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
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bioRxiv - Cell Biology 2024Quote: PA-JEB/β4 cells stably expressing Caskin2-BirA* fusion proteins were grown to 70%-80% confluence on 3 × 145 mm plates in biotin-depleted keratinocyte growth medium before they were treated with 50 μM biotin (Sigma-Aldrich #B4501) for 20 h at 37°C in DMEM/10% FCS ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant plasmids were transformed into TUNER (Novagen) cells in LB broth containing 10 μg/ml kanamycin at 37 °C shaking at 180 rpm ...
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bioRxiv - Immunology 2022Quote: ... DNAse I recombinant (Roche by Sigma Aldrich) and sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 μg/l recombinant mouse (Sigma-Aldrich) or human epithelial growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant 6His-ErbB3 was purchased from Sigma. The in vitro binding assay was conducted by incubating 1 μg/ml recombinant ErbB3 with 1 μg/ml recombinant Rabaptin5 in a stringent buffer containing 50 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... 103 IU recombinant mouse LIF (Millipore, ESG1107), 2mM L-glutamine (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... a recombinant HRV 3C protease (Sigma-Aldrich) was added to NSP7/NSP8 complex and incubated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Plant Biology 2022Quote: ... and submerged in 0−700 µM biotin (Sigma; B4501) solution in water ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µM final concentration biotin-tyramide (Sigma-Aldrich, SML2135) was added to the media ...
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bioRxiv - Cell Biology 2020Quote: ... biotin-HRP conjugated (Sigma A0185, mouse, WB:1/1000), anti-KAT2A/GCN5 (Cell Signaling Technology 3305 ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescent Atto 488nm-biotin (0.008 µg/sample, Sigma Aldrich) was used to label extracellular beads.
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bioRxiv - Biochemistry 2021Quote: ... 2.2mg (0.005 mmol) of Azide-PEG3-biotin (Sigma-Aldrich) was suspended in 10 µL of DMSO and added to the t-butanol:H2O mixture ...
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bioRxiv - Bioengineering 2020Quote: ... were conjugated to Atto-500 Biotin (Sigma Aldrich, USA) and were incorporated into the COL1/HA solution at a concentration of 0.1 mg ml−1 and used to identify the interface between the COL1 and COL1/HA regions ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with biotin (50 μM; Sigma-Aldrich) for indicated exposure times ...
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bioRxiv - Plant Biology 2022Quote: ... supplemented with 1% (w/v) biotin-free BSA (Sigma)) ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μl Biotin (33 μM, Sigma-Aldrich, B4639-100MG), 100 μl Pantothenate (17 μM ...
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bioRxiv - Immunology 2022Quote: ... then 55µL of hydrazide biotin at 50mM (Sigma, 21339), 25µL of 1M NaOAc ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin for click it reaction was purchased from Sigma. All primers and RNA sequences used in this study were purchased from Integrated DNA Technologies (IDT) ...
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bioRxiv - Cell Biology 2021Quote: ... biotin-16-deoxyuridine (bio-16-dUTP) (Sigma-Aldrich, USA) was substituted for the appropriate unlabeled dNTP in the following polymerase reaction mix ...
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bioRxiv - Microbiology 2020Quote: Biotin binding sites on streptavidin-coated beads (Millipore Sigma) were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μl Biotin (33 μM, Sigma-Aldrich, B4639-100MG), 100 μl Pantothenate (17 μM ...
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bioRxiv - Microbiology 2021Quote: ... falciparum cultures were incubated with 20 mM Biotin (Sigma) for 24-48 hours before harvesting ...
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bioRxiv - Biochemistry 2021Quote: ... medium was further supplemented with 50 μM biotin (Sigma), unless indicated otherwise.
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bioRxiv - Evolutionary Biology 2020Quote: ... The biotin-bound capture primers (Sigma-Aldrich, Gillingham, UK) were attached to biotinylated magnetic beads (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... or anti-Biotin-HRP (1:40,000, Sigma-Aldrich A0185) in biotin blocking buffer at 22°C for 60 minutes ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 4 µg/ml D-biotin (Sigma-Aldrich) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: D-Biotin (Millipore-Sigma, USA; cat. no. B4501-1G)
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 40 μM d-Biotin (Sigma) ingrown media for different time points (20 ...
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bioRxiv - Cell Biology 2023Quote: ... BSA-Biotin (cat: A8549) was obtained from Sigma-Aldrich. Tween 20 (cat ...
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bioRxiv - Biochemistry 2023Quote: D-Biotin (Millipore-Sigma, USA; cat. no. B4501-1G)
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bioRxiv - Synthetic Biology 2023Quote: ... substituting the Biotin-7-dATP with dA (Sigma-Aldrich). Anticipating a noisier response from the full genetic circuits ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5µM Biotin-PEG4-alkyne (Sigma-Aldrich, Cat. No. 764213). The reactions were incubated at room temperature for 1.5Hr and then precipitated with TCA as described above to remove excess click chemistry reagents ...
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bioRxiv - Biophysics 2023Quote: ... to which helicase (as indicated) and biotin (Sigma-Aldrich) were added to 100 µM (acting as a trap for free streptavidin ...