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Citations for Millipore Sigma :
201 - 250 of 8860 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... Activation of primary B-cells was induced by the addition of 10 μg/ml LPS (from E. coli, O111:B4, Sigma-Aldrich, Cat#L4391). Ramos cells were authenticated by Eurofins Genomics (Ebersberg ...
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bioRxiv - Neuroscience 2024Quote: ... pups PND 8-9 were given an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 40 μg/kg; E. coli O111:B4, Sigma; dissolved in sterile saline) or vehicle (saline) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli (Millipore) expressing human LC3B-WT ...
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bioRxiv - Molecular Biology 2021Quote: ... coli (Novagen) for expression of recombinant protein or ΔabmR Mtb for transcriptional activity assays.
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bioRxiv - Bioengineering 2021Quote: ... coli (Novagen) and selected on LB ampicillin with kanamycin B (15 μg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... coli (Novagen). Bacterial cultures were grown to an OD600 of 0.8 at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... coli (Sigma) at 20 ng/mL for NF-κB induction were used as positive controls.
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bioRxiv - Neuroscience 2021Quote: ... coli (Sigma). A batch of mice was used to run a comparative analysis of cortical gliosis in SW ...
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bioRxiv - Physiology 2022Quote: ... coli (Sigma L5293 ...
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bioRxiv - Immunology 2022Quote: ... coli (Novagen) to make high copy plasmids ...
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bioRxiv - Biophysics 2019Quote: ... coli (Novagen). Bacterial cultures were grown to an OD600 of 0.8 at 37°C and then expression was induced with a final concentration of 1 mM of isopropyl β-D-1-thiogalactopyranoside and the growth temperature was reduced to 16°C for 14-16 hours ...
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bioRxiv - Plant Biology 2020Quote: ... coli (Millipore), cultured in LB and induced to express proteins by adding 1 mM IPTG ...
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bioRxiv - Biophysics 2020Quote: ... coli (Novagen) was predominantly used ...
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bioRxiv - Systems Biology 2019Quote: ... coli (Novagen) in minimal (M9 ...
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bioRxiv - Plant Biology 2019Quote: ... coli (Novagen). The expression of the recombinant protein (ending with DSDVD in C-ter ...
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bioRxiv - Microbiology 2021Quote: ... coli (Millipore) for protein expression ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... coli (Millipore) were transformed with the expression vectors ...
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bioRxiv - Immunology 2020Quote: ... coli (Novagen). The bacterial cell pellets were resuspended in the lysis buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Novagen) transformed with pET_-7GFP ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Novagen) was performed as described (Geli et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli (Novagen) by heat-shock and plated on LB with the appropriate selection ...
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bioRxiv - Neuroscience 2022Quote: ... coli (Sigma) in 10 mM ammonium acetate buffer pH 5.3 ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) was transformed with each expression vector and grown in 50 ml LB broth containing 100 µg/ml ampicillin at 37°C with shaking until A600 reached between 0.6 and 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) grown in Terrific Broth media ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Novagen) by heat shock ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Novagen). 250 mL to 1 L bacteria cultures were grown in LB broth with ampicillin (100 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli (NOVAgen.1 L bacteria cultures in LB broth containing ampicillin (100 μg/mL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (Novagen) and purified by affinity and size exclusion chromatography ...
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bioRxiv - Microbiology 2023Quote: ... coli (Novagen) carrying a modified pET28a expressing either EHEC FruK-C-His or EHEC Cra-C-Flag were grown in 150 mL LB supplemented with kanamycin (50 µg mL−1 ...
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bioRxiv - Immunology 2023Quote: ... coli (Novagen). Cells were lysed with an M-110P microfluidizer (Microfluidics ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Sigma) measured via Amplex red uricase activity assay (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... coli (Novagen) and coselected on ampicillin (100 µg /ml ...
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bioRxiv - Immunology 2023Quote: ... E-64d (Sigma), or 0.5% DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Molecular Biology 2020Quote: ... coli ProQ protein or its N-terminal domain (NTD) were cloned into pET15b vector (Novagen) using BamHI restriction site (Supplemental Table S2) ...
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of recombinant protein was induced by 0.2% (w/v) L-Arabinose (Sigma Aldrich). The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Neuroscience 2021Quote: ... The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220; Sigma-Aldrich Japan), eluted with FLAG peptide (A3290 ...