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Citations for Millipore Sigma :
451 - 500 of 8860 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... coli extract (100500P, Sigma) was dissolved in chloroform and the organic solvent was evaporated using the first nitrogen stream ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) (Novagen) cells and were induced with 0.2 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biophysics 2023Quote: ... coli competent cells (Novagen) with appropriate antibiotics ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta strain (Novagen). The cell pellet was resuspended and lysed by sonication on ice in 100 mL of lysis buffer containing 25 mM Tris (pH 7.5) ...
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bioRxiv - Physiology 2023Quote: ... coli Rosetta 2 (Novagen), a single colony was inoculated into a 5-ml starter culture and grown overnight before being added to 500 ml Auto-Induction media (Formulation (g/l ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21 (DE3) (Novagen) was transformed with the resulting recombinant plasmids (pET28a-MurA ...
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bioRxiv - Genetics 2023Quote: ... coli BL21Rosetta cells (Novagen) harboring the pRARE plasmid (Novagen) ...
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bioRxiv - Biochemistry 2023Quote: ... coli bacteria (Sigma-Aldrich) following an earlier protocol (Herrmann et al ...
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bioRxiv - Biophysics 2023Quote: ... coli cells (Sigma Aldrich). For RGG constructs (RGG2 and RGG2-GFP) ...
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bioRxiv - Genetics 2023Quote: ... coli cells (Novagen 70951) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) (Novagen). Transformed cells were first cultured in LB medium at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 DE3 (Novagen) and induced overnight by 0.2 mM isopropyl β-D-thiogalactoside at 16 °C in the LB medium ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Rosetta2 (EMD Millipore) and plated on LB plates containing Ampicillin (Amp ...
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bioRxiv - Neuroscience 2023Quote: ... coli (Millipore EMD, #71397). Ribonucleoprotein complexes were placed at 37°C for 15 minutes prior to injection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli competent cells (Novagen). Cultures were grown to an OD of 0.8 in LB media ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coli (Novagen, Madison, WI) and FABP1 purification and delipidation were optimized and conducted as described in detail in Supplemental Materials ...
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bioRxiv - Molecular Biology 2024Quote: ... coli competent bacteria (Novagen) and transformed cells were selected on agar plates using Carbenicillin (100µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... coli (Sigma-Aldrich, L2018) for 8 hours and ATP (10nM)(InvivoGen ...
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bioRxiv - Biochemistry 2024Quote: ... coli competent cells (Novagen) with appropriate antibiotics ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... coli LPS (5 mg/kg) (Escherichia coli serotype O111:B4; Sigma, MO, USA). For more information ...
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bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... coli genes for proteins bS6 and bL9 were PCR-amplified and cloned individually into the pET28(a) plasmid (Novagen). Recombinant single-cysteine proteins were then expressed ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... pronase E 1 mg/ml (Sigma), and DNAse I 50 U/μl (Applichem ...
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bioRxiv - Cancer Biology 2019Quote: ... Cyclin E (1:1000, Sigma Aldrich), V5 (1/500 ...