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Citations for Millipore Sigma :
101 - 150 of 8860 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... LPA and recombinant Src protein were purchased from Sigma-Aldrich, while recombinant human IDO1 protein was obtained by Giotto Biotech ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2020Quote: ... or spectinomycin (100 mg/L for E. coli or 400 mg/L for S. venezuelae, Sigma Aldrich, USA) All bacterial strains and plasmids used in this work are listed in Table 2 and are available through the public instance of the JBEI registry ...
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bioRxiv - Immunology 2019Quote: ... Cells were either unstimulated or challenged with LPS (1ug/mL; LPS from E. coli O111:B4; Sigma-Aldrich) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... DCs were treated for 18h with Fh12 (2.5µg/ml or 1µg/ml LPS (E. coli 0111: B4; Sigma-Aldrich). In the inhibition experiments ...
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bioRxiv - Immunology 2020Quote: ... M1 macrophages were then kept unstimulated or stimulated with 100 ng/mL LPS (E. coli 0111:B4; Sigma) for 4h or 8h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BMDMs were also stimulated with lipopolysaccharide (LPS; standard LPS, E. coli 0111: B4; Sigma-Aldrich, 500 ng/mL) plus Nigericin (tlrl-nig ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full-length PRC1 was expressed for 3.5 hours at 18°C after induction with 0.5 mM IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Immunology 2021Quote: ... The same volumes of PBS and LPS (20 μg/mouse, from E. coli O114:B4, Sigma-Aldrich, Shanghai, China) were used as negative control and positive control ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
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bioRxiv - Immunology 2019Quote: ... 30 minutes before being challenged with 40 mg kg−1 LPS (E. coli, serotype 0111:B4, L-2630, Sigma). Mice were euthanized 3 hours after LPS challenge for blood collection ...
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bioRxiv - Neuroscience 2023Quote: ... 13 weeks old mice were injected intraperitoneally (i.p.) with 5□mg/kg of LPS (L2880, E. coli O55:B5, Sigma) or endotoxin-free PBS between 1 p.m ...
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bioRxiv - Bioengineering 2024Quote: ... were injected intraperitoneally (i.p.) with PBS or 10 mg/kg of lipopolysaccharide (LPS from E. coli O111:B4, 1 µM; #L2630, Sigma). After 8 h ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Acute inflammatory-type macrophages were induced by treating cells with 10 ng/mL lipopolysaccharide (from E. coli 055:B5, Sigma) for 3.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... vehicle (HBSS) or 100 ng/mL lipopolysaccharide (LPS E. coli O111:B4; 500,000 EU/mg; Sigma-Aldrich; St. Louis, MO) was administered for 3 hrs in Hx or Nx ...
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bioRxiv - Biophysics 2022Quote: ... coli optimized gene sequence for expression of S.c. Cdc6 in E. coli (a generous gift of Dr. Megan Davey, Western University, Canada) was cloned into pET11 (Novagen). The Cdc6 gene was then subcloned into pGEX-6P-1 (GeneScript ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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bioRxiv - Molecular Biology 2020Quote: ... and calmodulin was ordered from Genewiz (codon optimized for expression in E. coli) and cloned into the NcoI and EcoRI sites of pET-28a (Novagen).
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753 ...
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bioRxiv - Immunology 2023Quote: ... were plated in P96 plates and infected overnight with BCG at an MOI of 10 or Mtb at an MOI of 1 or stimulated overnight with 100 ng LPS (from E. Coli 011: B4, Sigma) and 10 µM nigericin sodium salt (Sigma ...
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bioRxiv - Immunology 2023Quote: ... mice were injected intraperitoneally (i.p.) or intravenously (i.v.) with a single dose of LPS (E. coli O111:B4; Sigma Cat#L2630) 2.5 mg/kg ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...