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Citations for Millipore Sigma :
351 - 400 of 3795 citations for Plasma kallikrein KLKB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: Plasma insulin was determined using a commercial kit (Merck Millipore) on a MAGPIXTM Multiplex reader and processed using Bio-Plex ManagerTM MP.
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bioRxiv - Cancer Biology 2019Quote: ... and loss of plasma membrane integrity (7-AAD staining; Sigma) were repeated every 15 min on a FACSCanto II instrument and analyzed with FACSDiva software ...
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bioRxiv - Bioengineering 2021Quote: ... thrombin from bovine plasma (T4648, Sigma-Aldrich, St. Louis, MO), 6-aminocapuroic acid (6-AA ...
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bioRxiv - Cell Biology 2021Quote: ... thrombin from bovine plasma (50 U/ml, Sigma- Aldrich, USA) was added to the mixture at a thrombin:total mixture volume ratio of 0.016:1 ...
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bioRxiv - Cell Biology 2020Quote: ... at 12μg/ml and Fibronectin from bovine plasma (F1141, Sigma) at 1μg/ml for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Heat-inactivated plasma and exogenous guinea pig complement (EMD Millipore) were incubated with the target strain ...
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bioRxiv - Neuroscience 2020Quote: ... radioactive plasma samples were loaded on ultrafiltration tubes (Millipore Centrifree) and centrifuged at 1500g for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were treated with fibronectin from bovine plasma (Sigma-Aldrich, F1141) and allowed to dry ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma membranes were visualized by trypan blue (0.05%; Sigma-Aldrich) in HBSS+/+ ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 4 ug/mL Fibronectin bovine plasma (Sigma-Aldrich F1141). Cells were allowed to recover overnight in complete medium.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and citrate plasma were purchased from Sigma-Aldrich (Darmstadt, Germany) and 4-nitrocatechol sulfate from ChemCruz (Santa Cruz Biotechnology) ...
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bioRxiv - Bioengineering 2023Quote: ... Inserts were coated with bovine plasma fibronectin (Sigma-Aldrich, F1141) at 50 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... and Thrombin from murine plasma were purchased from Sigma-Aldrich.
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bioRxiv - Cell Biology 2024Quote: ... fibronectin (bovine plasma fibronectin, F4759, Sigma Aldrich, ∼ 450 kg/mol); fibronectin-HiLyte488 (FNR02 Cytoskeleton ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... HEK293 cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cell Biology 2019Quote: ... and coated with 50 µg/ml bovine plasma fibronectin (F1141, Sigma) in a total volume of 250 µl overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: Samples from purified rFIX and plasma derived FIX (pdFIX) (F0806, Sigma) were prepared by performing in-solution digestion ...
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bioRxiv - Bioengineering 2021Quote: ... fibrinogen and thrombin from bovine plasma were all purchased from Sigma-Aldridge ...
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bioRxiv - Molecular Biology 2019Quote: Plasma RNA was extracted using TRI Reagent BD (T3809, Sigma Aldrich), in accordance with manufacturer’s instructions ...