Labshake search
Citations for Millipore Sigma :
501 - 550 of 3795 citations for Plasma kallikrein KLKB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasma samples for glucose analysis were derivatized first with 30 µl ethylhydroxylamine (Sigma) at 20 mg/ml in pyridine for 20 min at 80°C ...
-
bioRxiv - Physiology 2022Quote: ... plasma was deproteinated using a Microcon-10kDa centrifugal filter unit (Millipore Sigma, #MRCPRT010). For glutathione (GSH ...
-
bioRxiv - Neuroscience 2024Quote: ... Plasma samples were then filtered with a 0.8 µm filter (SLAA033SB, Merck Millipore), aliquoted into a microcentrifuge tube ...
-
bioRxiv - Physiology 2023Quote: ... and plasma insulin (n=5) was analyzed by ELISA (EZRMI-13K, Sigma Aldrich), according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... Plasma leptin and insulin levels were measured by murine ELISA kits (Merck Millipore, Darmstadt ...
-
bioRxiv - Molecular Biology 2024Quote: ... Plasma insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Millipore, Burlington MA).
-
bioRxiv - Physiology 2024Quote: ... Plasma active (acylated) ghrelin was measured by mouse/rat ELISA (EZRGRA-90K, Millipore). Plasma total ghrelin was measured by mouse/rat ELISA (EZRGRT-91K ...
-
iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
-
bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
-
bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
-
bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
-
bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
-
bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
-
bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
-
bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
-
bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
-
bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
-
bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
-
bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
-
bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
-
bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
-
bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
-
bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
-
bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
-
bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
-
bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
-
bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... were plasma cleaned and immediately coated with 0.5 mg/mL Concanavalin A (Sigma, C2010). Synchronized zoospores were washed three times with Bonner’s salts (10.27 mM NaCl ...
-
bioRxiv - Physiology 2021Quote: ... and plasma insulin (pg/ml) was measured via Luminex multiplex technology (Millipore, Billerica, MA).
-
bioRxiv - Immunology 2021Quote: ... IgG depletion from plasma was performed using the Albumin IgG Depletion Spintrap from Millipore-Sigma (cat # GE28-9480-20 ...
-
bioRxiv - Genomics 2021Quote: ... Plasma samples were filtered using Amicon Ultra Centrifugal Filters (3k molecular weight cutoff, Millipore) to remove proteins and lipids ...
-
bioRxiv - Cell Biology 2022Quote: ... and then incubated for 1 hr with a fibronectin (FN; Bovine plasma, Sigma-Aldrich) solution having a total concentration of 35 μg/mL (dissolved in 0.1 M NaHCO3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... coverslips were coated with 2.5 µg/mL bovine plasma fibronectin (Millipore Sigma, F1141-2MG) diluted in phosphate buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... and plasma cholesterol levels using a colorimetric cholesterol quantitation kit (Sigma-Aldrich, MAK043-1KT), according to the manufacturers’ protocols.