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Citations for Millipore Sigma :
601 - 650 of 3795 citations for Plasma kallikrein KLKB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... In order to functionalize the plasma treated cantilevers with concanavalin A (conA) (C2010, Sigma-Aldrich), the cantilevers were placed in a phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2020Quote: ... Plasma and blood cells were separated using Histopaque-1077 according to the manufacturer’s protocol (SIGMA). Briefly ...
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bioRxiv - Immunology 2022Quote: Deactivated saliva and plasma specimens were first passed through 10-kDa cutoff filters (Microcon, Millipore). The filtrates and the remaining materials on filters were subjected to peptidomics and proteomics analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... plasma-activated and silanized with vapored trichloro (1H,1H,2H,2H-perfluorooctyl) silane (Sigma-Aldrich) overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Around 20k cells/cm2 were seeded on fibronectin-(from bovine plasma, 5μg/ml, Sigma Aldrich) coated plates ...
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bioRxiv - Pathology 2020Quote: ... Plasma LH and FSH concentrations were determined using a multiplex assay (MPTMAG-49K, Merck Millipore).
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bioRxiv - Cell Biology 2020Quote: Total RNA from cell cultures and from plasma was extracted with Trizol reagent (#T9424, Sigma) and 0.5–1 μg was retrotranscribed using the TaqMan reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... the glass-bottom dishes were coated with 10 µg/ml bovine plasma fibronectin (FN; Sigma).
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bioRxiv - Developmental Biology 2022Quote: ... by measuring plasma fluorescence after oral gavage of 4-kDa FITC-dextran (Sigma-Aldrich, #46944) diluted in PBS (pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Blood samples were prepared as plasma with EDTA (Sigma-Aldrich Chemical Company, Milwaukee WI, USA) and centrifuged at 3000 g for 10 minutes (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 7,4’-dihydroxyflavone and the plasma membrane microsomes using nitrocellulose filters (0.45 mm pore-size; Millipore). The transport assays were performed with microsomes corresponding to 520 ng·μl-1 protein concentration mixed with transport buffer (10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... The plasma insulin concentrations were measured using an RIA assay (Millipore, St. Charles, MI, USA). Leptin concentrations were determined by ELISA (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Blood samples were prepared as plasma with EDTA (Sigma-Aldrich Chemical Company, Milwaukee WI, USA) and centrifuged at 3000 g for 10 minutes (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: Sarstedt Lumox dish 35 chambers were coated in Fibronectin from bovine plasma (Sigma F1141-1MG) diluted 1:1 with DMEM ...
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bioRxiv - Physiology 2023Quote: Glycerol was measured in mouse plasma samples using the Glycerol Assay Kit (MAK117, Sigma-Aldrich) following the procedure recommended by the manufacturer ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: Plasma FGF21 and insulin were assayed using the rat/mouse FGF21 ELISA kit (EMD Millipore) and the ultrasensitive mouse insulin ELISA kit (Crystal Chem) ...
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bioRxiv - Systems Biology 2024Quote: ... Plasma leptin was determined using an ELISA kit (EZML-82K; Millipore Sigma, St. Louis, MO) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Plasma renin activity was measured by fluorometric assay using the Renin Assay Kit (Sigma Aldrich). Colorimetric and fluorimetric readouts from these assays were attained using a FLUOstar Omega plate reader (BMG Labtech).
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...