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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Initiator 2,2-azobis(2-methyl-propionitrile) (AIBN; >98%; Sigma-Aldrich) was recrystallized from methanol (>99.9% ...
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bioRxiv - Physiology 2021Quote: ... N-Methyl-2-pyrrolidone (NMP) was from Sigma-Aldrich (328634). Intraperitoneal injections (IP ...
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bioRxiv - Neuroscience 2021Quote: Metyrapone (2-Methyl-1,2-di-3-pyridyl-1-propane; Sigma), a steroid 11β-inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... Biotinylated hyaluronan-binding protein (HABP) wassupplied by Millipore-Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated hyaluronic acid binding protein (Sigma-Aldrich, Singapore) were diluted in blocking buffer and incubated with samples overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Hyaluronic Acid Binding Protein (HABP; Millipore-Sigma, 385911), Ki67 (1:50 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... Hyaluronic Acid Binding Protein (HABP; Millipore-Sigma, 385911), Ki67 (1:50 dilution ...
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bioRxiv - Synthetic Biology 2023Quote: ... Single-strand binding protein from Sigma-Aldrich (S3917) was added at the final concentration (in Mixture A+B reaction ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked with 2% BSA (Sigma) and 2% donkey serum (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Nonspecific binding was blocked with 2% BSA (Sigma) and 2% donkey serum (Sigma ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: For treatment with methyl 2-benzimidazole carbamate (MBC; Sigma 45368-250M), we washed the cell cultures twice with the same growth media at the appropriate concentration of MBC ...
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bioRxiv - Neuroscience 2021Quote: ... and flash-frozen in liquid nitrogen-cooled 2-methyl butane (Sigma) prior to serial sectioning at 25μm thickness onto Superfrost Plus glass slides (Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629). Cultures were grown for another 30 min at 37 °C to reach OD600=0.8-1 ...
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bioRxiv - Immunology 2021Quote: ... containing cryomolds and immediately frozen in 2-methyl-butane (Sigma-Aldrich). 5μm frozen sections of the tissues were made using the cryostat and layered on Superfrost™ Plus Slides (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... compound (Tissue-Tek) in liquid nitrogen-cooled 2-methyl butane (Sigma). Ten micron sections were melted onto PEN-membrane glass slides (Leica ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were flash-frozen in 2-methyl-butane (Sigma-Aldrich M32631) at −150°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 4%UA/2% Methyl Cellulose (Sigma Aldrich, Gillingham, UK) at a 1:9 ratio on ice for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 3-Methyl-1-phenyl-2- pyrazolin-5-one (Edaravone; Millipore 443300) was resuspended in ethanol at 500mM and heated at 65°C for solubilization ...
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bioRxiv - Neuroscience 2022Quote: ... frozen in 2-methyl butane (Sigma-Aldrich, St. Louis, MO, USA) over the fumes of liquid nitrogen ...
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bioRxiv - Genomics 2024Quote: Isopentane (2-methyl butane; EMD Millipore/MilliporeSigma, cat. no. MX0760-1)
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Biotinylated hyaluronan-binding protein (HABP) was supplied by Millipore-Sigma ...
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bioRxiv - Microbiology 2020Quote: ... filtered through a 0.45μm low protein-binding filter (Millipore), and used to transduce Vero cells ...
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bioRxiv - Cell Biology 2021Quote: Biotinylated hyaluronan-binding protein (HABP) was supplied by Millipore-Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... using biotynilated HA-binding protein (385911, Sigma Aldrich, USA) and biotynilated GSLII (B-1215 ...
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bioRxiv - Immunology 2023Quote: ... filtered through 0.45 μm low- protein-binding filters (Millipore) and concentrated via 16 hours centrifugation at 3000 x g at 10 °C ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) were purchased from Sigma-Aldrich. Vismione H and madagascine were obtained from PGE2 fraction of Psorospermum glaberimum as previously described (Gallé ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... CpG 1826 (0.25 mg/mL) (Sigma-Aldrich) or Polyriboinosinic:polyribocytidylic acid (Poly I:C ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2,2,2-tribromoethaol 1.25%, 2-methyl-2-butanol 0.78%; 20 µL/g, i.p.; Sigma Aldrich) and transcardially perfused with PBS followed by 4% paraformaldehyde in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... which was prepared as a 100% (w/v) stock solution in 2-methyl-2-butanol (Sigma), diluted 1:40 in PBS ...
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bioRxiv - Biophysics 2020Quote: ... and 2% (w/v) N,N-methyl-bis-acrylamide (Sigma-Aldrich, M1533) were mixed in PBS on ice and vortexed carefully ...
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bioRxiv - Systems Biology 2019Quote: ... Methyl methacrylate contains hydroquinone as stabilizer (Sigma-Alderich, CAS. 1310-73-2)
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bioRxiv - Biophysics 2019Quote: ... The (-)-1-methyl-2-(3-pyridyl)pyrrolidine was purchased from Sigma-Aldrich Brasil Ltda (Sao Paulo ...
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bioRxiv - Developmental Biology 2022Quote: ... or a scrambled 2⍰-O-Methyl RNA sequence (both custom-made, Sigma). The cells were harvested in PBS ...