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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-methyl-2-propanethiol (Sigma 109207), 0.01% acetophenone (Sigma W200910) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2- methyl-2-thiazoline (Sigma M83406), 1% citronellol (Sigma W230915) ...
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bioRxiv - Cell Biology 2020Quote: ... 2% methyl cellulose (Sigma-Aldrich), 1% pen/strep antibiotics and 1% NEAA ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Neuroscience 2019Quote: ... dissolved in 2-methyl-2-butanol (Sigma Aldrich) 2.5% v/v)] ...
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bioRxiv - Neuroscience 2024Quote: ... 2-methyl-2-thiazoline (2MT; M83406, Sigma Aldrich) presented in a Plexiglas arena (43 × 27 × 19 cm ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% 1-methyl-2-pyrrolidone (Sigma), 40% polyethylene glycol 400 (PEG ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-methyl-1,4-BQ (Sigma-Aldrich) and freshly synthesized 2-methoxy-3-methyl-1,4-BQ (see below).
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Cell Biology 2020Quote: ... sodium-3-methyl-2-oxobutyrate (ketovaline, Sigma), 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine ...
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bioRxiv - Neuroscience 2020Quote: ... frozen in 2-methyl-butane (Sigma-Aldrich) and stored at −80°C.
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bioRxiv - Microbiology 2023Quote: ... 2-methyl-1,4-naphthoquinone (menadione) (Sigma-Aldrich), Vitamin K1 (phylloquinone ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-methyl-2-oxovaleric acid (KIC) (Sigma), and Sodium 3-methyl -2-oxobutyrate (KIV ...
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bioRxiv - Genomics 2022Quote: ... The presence of MeCP2 was assayed by western blotting using anti-MeCP2 monoclonal antibody M6818 (Sigma) using IR-dye as a secondary antibody (IRDye 800CW donkey anti-mouse ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-Methoxy-3-methyl-1,4-BQ was synthesized as follows: 2 mmol 2-Methoxy-3-methyl-1,4-hydroquinone (Sigma Aldrich) and 2 mmol NaIO4 were mixed in a 50 ml round-bottom flask and 10 ml CH2Cl2 and 5 ml of water were then added ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hyaluronic acid binding protein (Millipore) was used at 10 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... and N-methyl-2-pyrrolidone (NMP, Sigma-Aldrich) at a 1:1 volume ratio was used with the pneumatic atomiser and a tip size of 300 μm ...
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bioRxiv - Microbiology 2022Quote: ... 5-Methyl-2-furyl methanol (Sigma-Aldrich CDS003383), Lepidine (Sigma-Aldrich 158283) ...
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bioRxiv - Cancer Biology 2023Quote: ... and Sodium 3-methyl -2-oxobutyrate (KIV) (Sigma) were used to prepare branched-chain ketoacid (BCKA ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were then washed 3x times with ribosome binding buffer and bound mannosylated molecules were eluted with ribosome binding buffer containing 200mM methyl-α-D-mannopyranoside (Sigma-Aldrich, M6882). The eluted fractions were then subjected to SDS-PAGE and immunoblotting with antibodies.
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bioRxiv - Neuroscience 2020Quote: ... in 5 mL of 2-methyl-2-butanol (Sigma Aldrich, #240486). Mice were deeply anesthetized using 15 µL of Avertin 2.5% per gram of body weight ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Bioengineering 2023Quote: ... 0.15 M 2-amino-2-methyl-1-propanol (AMP) (A65182, Sigma-Aldrich) in UPW ...
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bioRxiv - Bioengineering 2023Quote: ... HA (HA binding protein, #385911, Sigma Aldrich), αSMA (#19245S ...
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bioRxiv - Neuroscience 2022Quote: ... and ≥99% 4-methyl-3-heptanol and 99% 6-methyl-5-hepten-2-one from Sigma-Aldrich (Item numbers M48309 and M48805-100ML ...
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bioRxiv - Cell Biology 2020Quote: ... 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine, Sigma) or a combination of all three (BCKAs) ...
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bioRxiv - Bioengineering 2021Quote: ... Initiator 2,2’-azobis(2-methyl-propionitrile) (AIBN; Sigma, >98%) was recrystallized from methanol (MeOH ...
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Bioengineering 2023Quote: Photoinitiator 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (HEPK, Sigma Aldrich) was dissolved into deionized water to obtain an approximately 0.077 wt% solution ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-MECP2 (Sigma-Aldrich, M7443, 1:500), rabbit anti-RFP (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-MeCP2 (rabbit, 1/200, Millipore MABE328). Sections were incubated with appropriate secondary antibodies (488 anti-chicken ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... propyl acetate and 3-methyl-2-buten-1-ol (Sigma). Odorants were selected based on previous work using this task (Gu & Li ...
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bioRxiv - Biophysics 2022Quote: HK2p was dissolved in N-Methyl-2-pyrrolidone (MP) (Sigma) for channel experiments ...