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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and penicillin-streptomycin (final concentration 100 U penicillin ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 100 U/mL human interleukin-2 (Millipore Sigma; 11011456001), 2 ng/mL human interleukin-7 (Peptrotech ...
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bioRxiv - Microbiology 2022Quote: ... and human thrombin (final concentration 2 U/ml; Sigma) in 50 μl Hank’s Balanced Salt Solution supplemented with 400 μg/ml aprotinin (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... and 100 U/ml human IL-2 (Sigma-Aldrich). After initial activation by plate-bound 0.05 µg of anti-CD3 (homemade ...
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was filtered through a 0.2 µm low-protein binding syringe filter (Millex Durapore; EMD Millipore) and applied to glutathione Sepharose 4b resin (Cytiva ...
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bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Medium was collected after this period and filtered through 0.45μm ultra-low protein binding filter (Merck Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected and transferred to 0.22 μm hydrophilic low protein binding 96-well filter plates (Millipore) and centrifuged at 4000 rpm to remove residual cells ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Glycosidases were removed after incubation by filtration through 10 kDa protein-binding EZ™ filters (Millipore Corporation). N-glycans were then analysed by UPLC as previously described (36).
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Neuroscience 2020Quote: Human Embryonic Kidney Cells (HEK293) and HEK293 Flp-In T-Rex cells were grown and maintained in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 FlpIn mycBirA*FOPNL and HEK293 FlpIn mycBirA* cells were incubated for 24 h with 50 μM biotin (Sigma-Aldrich) in the presence or absence of 0.1 μg/ml tetracycline.
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bioRxiv - Cell Biology 2020Quote: ... NRCMs with serum-free DMEM no glucose media and ARCMs with serum-free media 199 for 16 h before treating with 4-methyl 2-oxopentanoic acid sodium salt (ketoleucine, Sigma), sodium-3-methyl-2-oxobutyrate (ketovaline ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µM rotenone for 60 min or for 2 h with 0.5 mM L-leucyl-L-leucine methyl ester (LLOMe; L7393, Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW, 114.17 g/mol; purity, ≥99%; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Neuroscience 2019Quote: ... and 0.1 g of odorant consisting of one of three different concentrations (0.1%, 1%, and 2% by weight) of methyl salicylate (Sigma-Aldrich) diluted in mineral oil (Fisher Chemical Paraffin Oil ...
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bioRxiv - Microbiology 2019Quote: ... All viruses were titrated on confluent BHK cells one day post-seeding in culture medium containing 2% (v/v) FBS and 0.5% (w/v) methyl cellulose (Sigma Aldrich). For DENV-2 titrations ...
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bioRxiv - Biochemistry 2020Quote: ... These were mounted in a drop of in 0.1 M phosphate buffer containing 1:1 of 2% methyl cellulose (Sigma-Aldrich) and 2.3 M sucrose on TEM grids with a carbon-coated Formvar film (Taab Laboratory Equipment Ltd) ...
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bioRxiv - Developmental Biology 2021Quote: ... 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385, Sigma-Aldrich) as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu ...
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bioRxiv - Neuroscience 2024Quote: ... and 2-(14-carboxytetradecyl)-2-ethyl-4,4-di methyl-3-oxazolidinyloxy (16-doxyl-stearicacid, 16-DSA, cat. no. 253596) from Sigma-Aldrich. 5-DSA (spin probe containing the nitroxide group attached on C5 that is located on the opposite terminal relative to the charged carboxyl fatty acid terminus ...
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bioRxiv - Microbiology 2021Quote: ... ScAb or mAb samples were incubated with the antigen in doubling dilutions and the binding was detected using anti-Human C Kappa horseradish peroxidase (HRP) conjugated secondary antibody (Sigma) (for scAbs ...
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bioRxiv - Immunology 2023Quote: ... DH270 UCA and anti-c-myc antibody binding were detected with 500 µl of 10 µg/ml goat-anti-human PE (Sigma) and 10 µg/ml goat anti-chicken Alexa 647 respectively ...
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bioRxiv - Immunology 2019Quote: ... and HEK293 and HEK293T cells in DMEM (Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and HEK293 cells with polyethylenimine (PEI, Sigma Aldrich). Details are described in the SI Appendix ...
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bioRxiv - Neuroscience 2022Quote: HEK293 culture (ECACC 85120602, Sigma-Aldrich, Munich, Germany), transfection and electrophysiological recordings were performed as described previously (Grimm et al. ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells were cultured in RPMI medium (Sigma) supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were treated with MG132 (Sigma-Aldrich) at a final concentration of 1 or 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... The infection was incubated for 2-3 hours at 37°C and then the viral inoculum was removed and replaced with a pH 7.2 methyl cellulose overlay medium (1% methyl cellulose [Sigma-Aldrich, MA]/5% FBS [Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Cell Biology 2020Quote: ... PQ (Methyl viologen dichloride hydrate, Sigma-Aldrich, #856177) was always freshly prepared and first adjusted to a 1 M stock in H2O ...