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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 3’-3’ linkage of CpG with some staples were applied by dibenzocyclooctyne (DBCO)-azide (Sigma, US) click chemistry (Fig ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Neuroscience 2022Quote: ... and 2% (v/v) of human serum (Sigma-Aldrich, cat. #H4522). BBB working medium was used for co-culture and assembloids ...
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bioRxiv - Immunology 2020Quote: ... cells were blocked with 2% human AB serum (Sigma-Aldrich, UK) for 10 minutes on ice and then stained with the relevant APC-labelled mAb or hIgG1 isotype (BioInvent ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... The concentration of guanidine-HCl was diluted to 2 M with 25 mM ammonium bicarbonate and filtrated with low binding 0.45 μm PVDF filters (Millipore). The Lys-C endopeptidase was added at a 1:100 enzyme to protein ratio to digest proteins at room temperature overnight ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... anti-MAR binding reagent (Millipore, MABE1076), anti-GST (clone 6G9) ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing medium was collected 46 and 65 hours post transfection and was filtered through 0.45 μM Millipore low-protein binding filter units (Millipore SCHVU02RE). Viral supernatant was concentrated by first using low speed centrifugation through 100 kDa MW cutoff Millipore Centricon 70 Plus cartridges to condense volume to < 4mL ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Developmental Biology 2021Quote: ... As control for iodide uptake and protein-binding measurements cells were also treated with 30µM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 µM methimazole (TPO inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Cancer Biology 2022Quote: ... Hydrogels were made permissive for protein binding by incubating them in 500 µl of Sulfo-SANPAH solution [0.2 mg/ml Sulfo-SANPAH (Sigma, 803332) and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Molecular Biology 2023Quote: ... the virus-containing supernatant was harvested by gentle pipetting and filtered with a low protein binding syringe filter (0.22 μM, EDM Millipore # SLGV033RB). The virus was frozen at -80 °C for long-term storage or transduced directly into the desired cells.
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bioRxiv - Immunology 2023Quote: ... Protein was harvested from cell culture supernatants and purified by binding to a nickel-nitrilotriacetic acid (Ni-NTA) column (Novagen) followed by elution with 1M imidazole containing 0.2% octyl β-D-glucopyranoside ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysate was incubated with RIP binding buffer containing magnetic beads coated with human Ago2 antibody or mouse IgG antibody (control) (Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: Wild type Hek293 cells were grown in DMEM (Sigma D6429) supplemented with 10% FCS ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... while HEK293 and A549 cells were cultured in DMEM (Sigma). Both were supplemented with 10% foetal calf serum (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Hela and HEK293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (PAN Biotech) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were cultured in high glucose DMEM (Sigma-Aldrich) containing 10% FBS (HyClone) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cell lines were cultured with MEM medium (Sigma-Aldrich) supplied with FBS (Funakoshi) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were incubated in culture with 1.5% DMS (Sigma) at room temperature for 7 minutes ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Sigma-Aldrich) coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: HEK293 derived-nsEVs were resuspended with dextran-FITC (Sigma-Aldrich) or seed saporin (SAP ...