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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... medium supplemented with 2% AB human serum (Sigma-Aldrich, Spain). At that moment ...
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bioRxiv - Cell Biology 2022Quote: ... REVs were incubated with 2 μM human hemopexin (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2020Quote: ... 300 U/mL Roche recombinant human IL-2 (Sigma-Aldrich) and 1 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Biochemistry 2020Quote: A reference standard of 38 fatty acid methyl ester standards was prepared by mixing a purchased 37 fatty acid methyl ester (FAME) standard (Restek, Bellefonte, PA, USA) and 450 μM methyl-nonadecanoate in MTBE (Sigma Aldrich) at a 1:9 ratio ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Microbiology 2022Quote: ... the medium was supplemented with a mixture of 3 branched-chain fatty acid precursors (100 μm of 2-methyl-butyrate, isobutyrate and isovalerate, Sigma-Aldrich) or straight fatty acid precursors (100 μm of methyl-butyrate ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977 ...
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bioRxiv - Microbiology 2023Quote: The enzymatic production of DsrC-trisulfide was performed inside a Coy anaerobic chamber (98% N2, 2% H2) at 60 °C using Zn-reduced methyl viologen (Sigma-Aldrich) as the electron donor (7) ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Neuroscience 2023Quote: We used the odorants methyl hexanoate (MHXE, CAS: 106-70-7) and 2-heptanone (HEPN, CAS: 110-43-0) (Sigma Aldrich). Odorant stimuli were generated with a custom-made stimulator optimized for temporal precision in binary mixtures [13] ...
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bioRxiv - Immunology 2024Quote: ... The small intestine sections were rolled up from distal-to-proximal into “swiss rolls.” The swiss rolls were embedded in Optimal Cutting Temperature (OCT) (Sakura Finetek) and flash frozen in 2-methyl butane (Sigma Aldrich) over dry ice ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Immunology 2020Quote: ... The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Genomics 2021Quote: ... Nonspecific binding was then blocked by incubation with PBS containing 2% BSA (fraction V, globulin free; Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Panc1 and HEK293 in DMEM (Sigma, Cat No. D1145), SH-SY5Y in 1:1 mixture of DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described [46 ...
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bioRxiv - Genomics 2022Quote: ... HEK293 were induced with 3 μg/ml doxycycline (Sigma) and 400 μg/ml of G418 (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were plated on poly-L-lysine (Sigma) coated coverslips in a 12-well plate and transfected with Kv4.1 or Kv4.2 cDNA ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells were cultured in low-glucose DMEM (Sigma) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Millipore) coated glass coverslips ...
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bioRxiv - Physiology 2019Quote: ... mice fasted overnight (14h) were injected with 250mg/kg of the norepinephrine synthesis blocker α-Methyl-DL-tyrosine methyl ester hydrochloride (AMT, Sigma-Aldrich) and immediately placed at 5°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2020Quote: ... the active molecule Acibenzolar-S-methyl (Sigma-Aldrich, Germany) was used ...
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bioRxiv - Cell Biology 2020Quote: ... or methyl-β-cyclodextrin (9.5 µM, Sigma-Aldrich C4555) were added to growth media at normal growth conditions for one hour prior to unroofing and fixation.
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bioRxiv - Cell Biology 2019Quote: ... N methyl-bis-acrylamide cross-linker (Sigma-Aldrich, M1533) in PBS (Table 1) ...
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bioRxiv - Neuroscience 2021Quote: ... colorimetric methyl-thiazolyl diphenyl-tetrazolium bromide (MTT; Sigma Aldrich). All purchased materials were used as received.
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bioRxiv - Neuroscience 2021Quote: ... Capsaicin (8-Methyl-N-vanillyl-trans-6-nonenamide, Sigma), RQ00203078 (Tocris ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 0.2 mM 3-isobutyl-1-methyl-xanthine (Sigma) to prevent resumption of meiosis ...
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bioRxiv - Bioengineering 2019Quote: ... 500 μM 1-methyl-3-isobutylxanthine (IBMX) (Sigma-Aldrich), 50 μM ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were cleared in methyl salicylate (Sigma Aldrich, M2047) for 30 min at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM methyl-cyclo-beta-dextrin (MßCD) (C4555, Sigma), or 1 μM Amphotericin B (A2942 ...
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bioRxiv - Biophysics 2020Quote: ... Propylene glycol methyl ether acetate (PGMEA) (Sigma-Aldrich, 537543) was then used to remove the unexposed SU8 from the wafer ...
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bioRxiv - Bioengineering 2020Quote: CDDO-methyl (Sigma Aldrich, St. Louis, MO. SMB00376-100MG), Synperonic-PE-P84 pluronic tri-block co-polymer (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and methyl-beta-cyclodextrin were purchased from Sigma-Aldrich Co (USA).
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bioRxiv - Cancer Biology 2020Quote: ... 5-methyl-THF was obtained from Sigma Aldrich (M0132). Isotopic tracers [U-13C] methionine (CLM-893-H) ...
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bioRxiv - Neuroscience 2020Quote: ... diacetyl and methyl-salicylate were obtained from Sigma Aldrich. ccBA and ccDA abbreviate undiluted (concentrated ...
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bioRxiv - Neuroscience 2019Quote: ... brains were mounted in fresh methyl salicylate (Sigma, 76631). In control experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.5 mM 3-methyl-1-isobutylxathine (Sigma-Aldrich) for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... then 250 μl methyl tert-butyl ether (Sigma-Aldrich) was added and the tubes were vortexed for 30 s ...