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Citations for Millipore Sigma :
151 - 200 of 1716 citations for Mayaro virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2020Quote: ... then the lysate was treated with RNAseA (Sigma-Aldrich) at 20 ug/ul for 30 min at 37 oC ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were incubated with FLAG agarose (Sigma-Aldrich), GFP-Trap Agarose (Chromotek ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... cleared cell lysates were incubated with glutathione agarose (Sigma) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were immunoprecipitated with FLAG M2 antibodies (SIGMA). The beads were washed with TBS/0.1% Triton X-100 for five times followed by elution with DYKDDDK peptide and processed for sample preparation.
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bioRxiv - Neuroscience 2019Quote: ... The lysate was treated with Benzonase nuclease HC (Millipore) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were treated with 2 mM 1,10-phenanthroline (Sigma), cross-linked with 0.5 mM DSP (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were incubated with anti-Flag (M2 clone; Sigma) or anti-m6A (Synaptic Systems ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysate was supplemented with 1 mM PMSF (Sigma). Proteins were then purified via a three strep purification protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were incubated with Anti-Flag (M2)-agarose (Sigma) for 4h to overnight at 4□ ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates were prepared using RIPA buffer (SIGMA; R0278) supplemented with protease inhibitors (Roche ...
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bioRxiv - Cell Biology 2021Quote: Tissue/cell lysates were prepared using RIPA (SIGMA #R0278) lysis buffer supplemented with cOmplete-ULTRA protease inhibitor (Roche #05892970001) ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-agarose or -sepharose (Sigma; GenScript) and Talon metal affinity resin (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: Protein lysates were harvested in RIPA buffer (Sigma, R0278) supplemented with protease inhibitors (Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 lysates were normalized to 180 nM fluorescien (Sigma) based on a standard curve ...
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bioRxiv - Microbiology 2021Quote: ... and cell lysates were collected in TRI reagent (Sigma) 24 hours post-treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were prepared in RIPA buffer (Sigma-Aldrich # R0278). Upon usage ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was treated with 250 units of Benzonase (Sigma) at 37°C for 30 min and then spun at 4300 x g for 30 min before being loaded onto an iodixanol gradient ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysate was extracted using RIPA lysis buffer (Sigma) containing protease inhibitor (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates containing p75 were supplemented with iodoacetamide (Sigma) to avoid post-lysate dimer disulfide formation ...
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bioRxiv - Molecular Biology 2022Quote: ... The remaining lysate was incubated with streptavidin beads (Sigma) for 30 min rotating at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Clarified lysates were then incubated on gluthathione-agarose (Sigma) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear lysates were treated with Benzonase (Merck-Millipore, 70664). Nuclear lysates were pre-cleared on 25 μL Dynabeads M-280 Sheep anti-mouse or antirabbit IgG (Thermo ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Denatured lysates were treated with Benzonase Nuclease (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates plus resin were transferred to spin columns (Sigma) and centrifuged to remove unbound protein ...