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Citations for Millipore Sigma :
1 - 50 of 1716 citations for Mayaro virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Microbiology 2019Quote: ... Anti-influenza A virus NP antibodies (Millipore), Anti-TNK2 (A12 ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were stored in aliquots containing 10% sucrose (Sigma) at −150°C ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Input virus was treated with benzonase (500U/mL, Sigma-Aldrich) before cell exposure to reduce nucleic acid contamination in the viral stock ...
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bioRxiv - Neuroscience 2021Quote: ... Concentration of virus was repeated on smaller Amicon Ultracels (Millipore) until desired high titer was reached ...
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bioRxiv - Cell Biology 2021Quote: ... with 60 μg of Tobacco etch virus protease (Sigma-Aldrich) added in the dialysis cassette to cleave the histidine tag ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of virus suspension and Polybrene (hexadimethrine bromide, Sigma) at a final concentration of 8 μg/ml were added ...
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bioRxiv - Microbiology 2023Quote: ... virus was filtered using a millipore filter (Millipore Sigma, SE1M179M6). On day of infection ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Plant Biology 2023Quote: ... and then the tobacco etch virus (TEV) protease (Sigma-Aldrich) was added to remove the purification tags ...
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bioRxiv - Microbiology 2023Quote: Swabs were stored in virus cultivation medium (Sigma-Virocult®). Archived swabs and organ samples were kept at-70 °C until final analyses ...
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bioRxiv - Microbiology 2023Quote: ... Virus propagation was performed in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal calf serum (Logan) ...
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bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
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bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
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bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...
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bioRxiv - Microbiology 2023Quote: ... the virus containing medium plus 8 µg/ml polybrene (Sigma #H9268) and the infected 293T cells were co-cultured with iSLK cells ...
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... The lysate was benzonase (Millipore, 70664) treated for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...
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bioRxiv - Genetics 2021Quote: ... Increasing amounts of virus and 8 μg/ml polybrene (hexadimethrine bromide, Sigma) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...