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Citations for Millipore Sigma :
451 - 500 of 1716 citations for Mayaro virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated with anti-flag M2 beads (Sigma-Aldrich, St. Louis, USA) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then treated with 50U/ml of Benzonase (Novagen #70664), for 30min in a 37°C water bath ...
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bioRxiv - Cell Biology 2019Quote: ... Pre-cleared cell lysates were incubated with anti-FLAG beads (Sigma, A2220) or anti-HA beads (Sigma ...
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bioRxiv - Genetics 2021Quote: ... The adjusted lysates were combined with anti-VSV-G agarose beads (Sigma) that had been washed once with IP buffer and reconstituted to a 50/50 bead/buffer slurry ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Developmental Biology 2021Quote: ... crude cell lysates were diluted in 2x Laemmli buffer (Sigma-Aldrich, #S3401) and denatured for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were loaded onto Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) and incubated for 2 h at 4°C with overhead shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysates were combined with 20μL of ANTI-FLAG M2 (Sigma-Aldrich) magnetic beads that had been pre-washed 2x with Buffer A then incubated for 2 hr on a rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Filtered lysates were incubated with the anti-FLAG beads (Sigma-Aldrich, M8823) for 2 hrs at room temperature (22 °C) ...
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bioRxiv - Immunology 2021Quote: ... Lysates were resolved on SDS-PAGE and transferred to PVDF membranes (Millipore), followed by immunoblotting as previously described (Haniuda et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... cleared lysates were incubated with Anti-FLAG® M2 Magnetic Beads (SIGMA) for 2h and washed two times in lysis buffer and one time in TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... lysates were incubated with anti-FLAG M2 ® magnetic beads (Sigma M8823). After incubation ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclear lysate was used for immunoprecipitation with Flag M2 Magnetic Beads (Sigma) for 2h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... whole cell lysates (WCLs) were precleared using protein A Sepharose (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Each cell lysate was incubated with ANTI-FLAG M2 affinity gel (Sigma) at 4°C overnight on a shaker at 10 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were sonicated and 10 % v/v β-mercaptoethanol added (Sigma-Aldrich). Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Subsequent lysates were supplemented with 10% Protease Inhibitor Cocktail (PrIC; Sigma-Aldrich), 1% Phosphatase Inhibitor Cocktail 2 (PhIC ...
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bioRxiv - Bioengineering 2020Quote: ... The lysate was filtered with Amicon 30MWCO filters (Millipore Sigma, Catalog #UFC8030) according to the manufacturer’s protocol and washed three times to exchange the buffer with the reaction buffer (45mM sodium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were then subjected to preclearing with Sepharose 4B beads (Sigma) at 4 °C for 1 h with agitation ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate was incubated with 25 U benzonase HC nuclease (Sigma-Aldrich) at 37 °C for 30 min before IP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated with Anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) for 4 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: Whole-cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Systems Biology 2021Quote: ... whole-cell lysates were incubated with Concanavalin A-Sepharose 4B (Sigma-Aldrich). Whole-cell lysates were rotated at 4°C for 1 hour with 100 ml of 50% Con-A slurry ...
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bioRxiv - Neuroscience 2020Quote: ... Cleared lysates were incubated with 10 μg of 2A8 antibody (EMD-Millipore) (Nelson et al. ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were subjected to SDS-PAGE and transferred to PVDF membrane (Millipore). Membranes were blocked in skim milk or BSA and probed overnight with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were vortexed 4 x 45 sec with glass beads (Sigma Aldrich), maintained on ice between pulses ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were incubated with anti-M2 FLAG-conjugated agarose beads (Sigma-Aldrich) at 10 ul bed volume per 1 mg of protein and incubated for 1 hour at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was applied to 0.5 mL of glutathione agarose (Sigma-Aldrich) per liter of culture for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Whole-cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then supplemented with 5-10 mM imidazole (Sigma #I5513), added to 1 ml of washed Ni2+-NTA agarose (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was then treated with Benzonase nuclease HC (#71206-3, Millipore) for 45 minutes at 37 oC ...
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bioRxiv - Pathology 2022Quote: ... The lysate was then treated with Benzonase nuclease HC (#71206-3, Millipore) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Then cell lysate was added to Streptavidin Agarose conjugated beads (Millipore, USA) and incubated for 2 h with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were placed on Sepharose 4B beads (Sigma-Aldrich CAT# 4B200) to clear for 30min at 4°C rocking ...
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bioRxiv - Microbiology 2021Quote: ... cellular lysates were treated prior immunoprecipitation with 10µg/ml of RNAseA (SIGMA) for 2 hours at 37°C.
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... was added to cell lysates prior to treatment with 50mM Iodoacetamide (Sigma) or 0.1M DTT (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were incubated with Anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) for 4 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of cell lysates were incubated with Flag-M2 (M8823, Sigma) or GFP (gtma ...
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bioRxiv - Microbiology 2020Quote: ... Sonicated cell lysates were subjected to immunoprecipitation with antibodies to CTCF (Millipore), SMC1 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... and lysates were incubated with anti-Flag-M2-agarose beads (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cleared lysate was incubated with anti-FLAG magnetic beads (Sigma-Aldrich, M8823) overnight with rotation at 4°C beads ...