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Citations for Millipore Sigma :
401 - 450 of 3833 citations for Malate dehydrogenase mitochondrial MDH2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Biochemistry 2020Quote: ... was added to reaction mixtures containing Pyruvate Kinase and Lactic Dehydrogenase enzymes (Sigma-Aldrich, Gillingham, United Kingdom) with 0.010-0.016 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 50 mM.min-1 pyruvate kinase and 50 mM.min-1 lactate dehydrogenase (as assayed by the manufacturer, Sigma). Ligases were diluted prior to assay as required in 50 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM phosphoenolpyruvate (PEP) (Molecula, 16921512) and 16 units/ml lactic dehydrogenase/pyruvate kinase enzymes (Sigma, P0294). 30 mL reactions were prepared in 384 well plates (Greiner ...
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bioRxiv - Microbiology 2019Quote: ... and alternative NADH dehydrogenase inhibitor flavone (2-Phenyl-4H-1-benzopyran-4-one, 2-Phenylchromone, Sigma-Aldrich) (Martins et al ...
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bioRxiv - Biochemistry 2022Quote: ... and commercial pyruvate kinase (PK) and lactate dehydrogenase (LDH) from Saccharomyces cerevisae (Sigma-Aldrich, Saint-Louis, USA) (Figure S2) ...
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bioRxiv - Cell Biology 2023Quote: ... Neuronal cytotoxicity was determined with the Lactate Dehydrogenase Activity Assay Kit according to the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... LDH activity was measured using the Lactate Dehydrogenase Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA). For histology ...
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bioRxiv - Plant Biology 2024Quote: ... both enzymes were combined with 10 units pyruvate kinase and 10 units lactate dehydrogenase (both Sigma-Aldrich) at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Biochemistry 2022Quote: ... and brain the mitochondrial isolation buffer was additionally supplemented with 0.2% bovine serum albumin (Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial samples from proteinase-K treatment were thawed and resuspended in RIPA buffer (Millipore #20-188) containing protease inhibitors (Sigma #P8340) ...
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bioRxiv - Physiology 2019Quote: ... was from BD Biosciences; citrate synthase (CS; SAB2701077) and mitochondrial fission protein 1 (FIS1; HPA017430) were from Sigma Aldrich; vitamin D receptor (D-6 ...
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bioRxiv - Neuroscience 2021Quote: Relative mitochondrial aconitase activity was quantified using the colorimetric Aconitase Activity Assay Kit from Sigma (#MAK051), following manufacturer’s instructions as previously described (Martelli et al. ...
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bioRxiv - Microbiology 2019Quote: ... and the mitochondrial GFP reporter strain AfS35 pJW103 (49) were maintained on malt extract (Sigma-Aldrich) agar plates supplemented with 2% (w/v ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... The standard mitochondrial stress test was performed with injection of FCCP (2 μM; Sigma-Aldrich, C2920), oligomycin (1 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μM rotenone and antimycin A (mitochondrial complex I and III inhibitors, respectively; Sigma-Aldrich). Cells were counted at the end of the experiments to normalize the OCR ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial fractions were subjected to various concentrations (0.25-1 mg/mL) of proteinase K (Sigma-Aldrich) for 30 mins at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Mitochondrial pellets were lysed in PBS with 1% n-dodecyl-β-D-maltoside (DDM; Sigma-Aldrich) and 1X protease-phosphatase inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Concentrations of the Mitochondrial stress assay inhibitors are as follows: 1µM oligomycin (Sigma-Aldrich, 75351-5MG), 2µM FCCP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial membrane potential was evaluated using the potentiometric dye tetramethyl rhodamine methyl ester (TMRM; Sigma Aldrich) at a final concentration of 10 nm ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2021Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma-Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Biochemistry 2020Quote: ... and 15 mM phosphoenolpyruvate in presence of a 4% v/v pyruvate kinase/lactate dehydrogenase enzyme mix (Sigma). Reactions were initiated by the addition of ATP to a final concentration of 25 μM - 6.4 mM ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Biochemistry 2020Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Lactate dehydrogenase activity in the basolateral medium was assayed using the Cytotoxicity Detection Kit (Sigma, cat. no. 4744934001). For the high control ...
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Low-cost drug discovery with engineered E. coli reveals an anti-mycobacterial activity of benazeprilbioRxiv - Synthetic Biology 2021Quote: ... 35 μL reaction cocktails of 0.05 U/mL L-alanine dehydrogenase and 20 mM β-NAD (Sigma N8129) in sodium bicarbonate buffer (pH 10.5 ...
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bioRxiv - Biophysics 2020Quote: ... The coupled enzymatic reaction contained ~2 units of pyruvate kinase/lactic dehydrogenase (rabbit muscle isolate mixture, Sigma Aldrich) 50 mM KPi ...
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bioRxiv - Cell Biology 2021Quote: ... and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control ...
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bioRxiv - Microbiology 2022Quote: ... 1.1 U.ml−1 lactate dehydrogenase and 0.8 U.ml−1 pyruvate kinase (rabbit muscle, P0294 from Sigma-Aldrich®) and 0.5 mg.ml−1 MtAPSK (all final concentrations ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Bioengineering 2023Quote: ... The conversion of pyruvate to lactate by lactate dehydrogenase was assessed with a pyruvate assay kit (Sigma, MAK332), and the color change of X-gal was observed in the channels and buffers for the β-galactosidase assay.
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bioRxiv - Biochemistry 2023Quote: ... and mixed 1:1 with a 2X assay solution of 40 U/mL lactate dehydrogenase (Sigma-Aldrich L3916), 1 M Tris (pH 9.8) ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...