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Citations for Millipore Sigma :
551 - 600 of 3833 citations for Malate dehydrogenase mitochondrial MDH2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Microbiology 2020Quote: ... measuring the decrease in absorbance at 340 nm 30 min after addition of 5 U of L-lactic dehydrogenase (from rabbit muscle, Sigma-Aldrich) and 4 U of pyruvate kinase (from rabbit muscle ...
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bioRxiv - Immunology 2020Quote: Lactate dehydrogenase (LDH)-based cytotoxicity levels were determined by evaluation of LDH release into the cell culture supernatants using Lactate Dehydrogenase Activity Assay kit (MAK066, Sigma-Aldrich). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... 20 uL of a 5x coupling solution containing 100 U/mL lactate dehydrogenase (product #L1254, Sigma-Aldrich, St. Louis, MO, USA), 500 U/mL pyruvate kinase (product #500-20 ...
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bioRxiv - Pathology 2020Quote: ... 120 or 180 min after the blood collection) was addressed spectrophotometrically using Lactate Dehydrogenase Activity Assay Kit (MAK066, Sigma Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the activity of an intracellular enzyme marker glyceraldehyde phosphate dehydrogenase (GAPDH) was assayed on each sub-proteome as per the manufacturer’s instructions (Sigma, St Louis). 500 ug of each sample was resuspended in 250 uL 0.5 M triethylammonium bicarbonate (pH 8.5 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... or ethanol (1.5 g/kg) with the alcohol dehydrogenase inhibitor pyrazole (68 mg/kg, i.p.; Sigma – Aldrich, P56607 – 6G; Figure 1A). The air – control group was also further divided into two separate groups that received either pyrazole (68 mg/kg in saline ...
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bioRxiv - Cancer Biology 2023Quote: ... To evaluate SDH activity cells were stimulated as described above and SDH activity was analyzed with Succinate dehydrogenase Activity Colorimetric Assay Kit (Sigma-Aldrich) according to the manufacturers′ instructions.
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was assayed between 20-24 hours post-treatment for lactate dehydrogenase (LDH) activity as a marker of cell death using a commercially available kit (Tox7, Sigma-Aldrich) as previously described (Aras et al. ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
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bioRxiv - Cell Biology 2019Quote: ... The standard assay medium used for all extracellular flux analyses and mitochondrial stress tests was unbuffered DMEM (SIGMA, D5030) without glucose ...
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bioRxiv - Biophysics 2021Quote: The spatial distribution of mitochondrial membrane potential within oocytes was measured with a potential-sensitive dye TMRM (Sigma Aldrich). Oocytes were cultured in AKSOM with 100 nM TMRM for 30 minutes before imaging ...
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bioRxiv - Biochemistry 2020Quote: Full length MRPP2 and His6-Δ39 MRPP1 lacking the predicted mitochondrial targeting sequences were cloned into pCDFDuet-1 (Novagen) vector (Liu et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondrial OCR was measured in a time course before and after the injection of 1.5 μM Oligomycin (Millipore, 495455), 2.5 μM FCCP (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and probed for the mitochondrial marker TOM20 with an anti-TOM20 rabbit polyclonal antibody (1:1000 dilution; Sigma-Aldrich) and anti-cytochrome c mouse monoclonal antibody (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 - 1 x 107 mitochondrial donors were suspended in 1:1 Percoll (Sigma-Aldrich, 65455-52-9) and cybrid generation buffer (D5030 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...