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Citations for Millipore Sigma :
501 - 550 of 3833 citations for Malate dehydrogenase mitochondrial MDH2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Microbiology 2020Quote: ... the cytosolic and mitochondrial fractions were 10-fold concentrated using Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Measurement of IDH activity on the enriched mitochondrial fractions was conducted using an IDH activity assay (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Mitochondrial or cellular hydrogen peroxide (H2O2) levels were measured in fibroblasts using mitochondria peroxy yellow 1 (MitoPY1) (Sigma) or Amplex Red reagent and horseradish peroxidase from the Amplex® Red Catalase Assay Kit (Molecular Probes) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with the following mitochondrial toxins for 16 h: 1.25 ng/mL oligomycin (Sigma-Aldrich # 75351), 50 µg/mL doxycycline (Clontech # 631311) ...
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bioRxiv - Physiology 2020Quote: ... Intracellular and mitochondrial superoxide generation was measured using dihydroethidium (DHE, 5 μM, 20 minutes) (Sigma-Aldrich, Dorset, UK) and MitoSOX Red mitochondrial superoxide indicator (5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondrial pellets were resuspended in 100 μl RIPA buffer supplemented with EDTA-free protease inhibitor cocktail (EMD Millipore) under constant agitation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 97062]) and then overlaid with 175μL of mitochondrial assay solution supplemented with 10mM sodium pyruvate (Sigma-Aldrich, P5280), 1mM malate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903] ...
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bioRxiv - Biochemistry 2024Quote: ... Mitochondrial depolarisation was induced by treatment with 10 μM AntimycinA and 1 μM Oligomycin (Sigma; prepared in DMSO) for 3 hrs ...
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial function and potential were analyzed by sequential injection of 1 μM oligomycin (ATP synthase inhibitor; Sigma-Aldrich), 0.6 μM Carbonyl Cyanide m-Chlorophenylhydrazone (CCCP ...
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bioRxiv - Microbiology 2023Quote: ... opening of the mitochondrial permeabilization transition pore (mPTP) was visualized by tetramethylrhodamine methyl ester perchlorate (TMRM) staining (Sigma). Cells were washed once with 1 x HBBS (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... The activity of creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma were measured using creatine kinase activity assay Kit (Sigma) and LDH activity assay kit (sigma ...
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bioRxiv - Bioengineering 2021Quote: ... the pellets were collected to examine the cell lysis interference using a Glucose-6-Phosphate Dehydrogenase kit (Sigma-Aldrich, USA). The details of EPS extraction and analytical methods for various EPS components (proteins ...
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bioRxiv - Plant Biology 2022Quote: AMPK3 and 4 activity was determined using a coupled enzyme assay with pyruvate kinase and lactate dehydrogenase (LDH, Sigma, P0294), measuring the production of NAD+ that results in absorption-decrease at 340 nm ...
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bioRxiv - Microbiology 2020Quote: Secreted lactate dehydrogenase (LDH) activity was measured as a correlate of cell death in culture supernatants using Cytotoxicity Detection KitPLUS (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: IDH activity assays were performed using the Isocitrate Dehydrogenase Activity Assay kit (MAK062) from Sigma-Aldrich (St. Louis, MS, USA) and monitored with a Synergy HT microplate reader (BioTek ...
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bioRxiv - Neuroscience 2022Quote: The cellular viability of the brain-like tissues was tested using a lactate dehydrogenase assay (LDH) (Sigma, cat.no. MAK066-1KT) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... and following the best approaches for optimizing activity (Moyes et al., 2021): Solution A (4 units mL-1 lactate dehydrogenase (L2500, Sigma), 5 units mL-1 pyruvate kinase (P1506 ...
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bioRxiv - Microbiology 2023Quote: ... 2012) using the reaction buffer (bicine-KOH 20 mM, pH=9.0 and 1 U/mL alcohol dehydrogenase, Sigma Aldrich A7011). The assay was carried out at 340 nm ...
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bioRxiv - Bioengineering 2023Quote: ... and creatine kinase (CK) levels were assessed using the Lactate Dehydrogenase Activity Assay Kit and Creatine Kinase Activity Assay Kit (Sigma), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate production was enzymatically quantified as NADH fluorescence (360 nm/460 nm) by the reverse reaction of L-lactate dehydrogenase (Rabbit muscle, L25005KU, Sigma) in a reaction containing 20 µL cell media ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...