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Citations for Millipore Sigma :
401 - 450 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 0.5% NP-40) supplemented with protease inhibitors and 1000 units of Benzonase (Sigma-Aldrich, #E1014-25KU) and rotated at 4℃ for 4h ...
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bioRxiv - Neuroscience 2024Quote: ... NP-40 STEN- and RIPA-lysis buffer were supplemented with protease inhibitor cocktail (# P8340, Sigma-Aldrich) and phosphatase inhibitor (# 4906845001 PhosStop™ ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 mM NaCl and 0.5 % NP-40 alternative) and benzonase (75 U per sample) (Sigma E1014). Samples were incubated for 15 min at 4 °C and centrifuged at 21000 g for 2 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Microbiology 2021Quote: ... The virus/antibody mixture was replaced with an overly media containing 1% CMC (Sigma) before incubation for 24 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... 1% NP-40 and 2 mM EDTA) supplemented with protease and phosphatase I and II inhibitors (Sigma–Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 mM EDTA and 0.5% NP-40) supplemented with phosphatase/protease inhibitor cocktail (MS-SAFE, Sigma-Aldrich) in a 4°C water bath using a sonicator (QSONICA ...
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bioRxiv - Cancer Biology 2020Quote: ... permeabilized with 0.5%-Triton-X100 for 5 minutes and blocked with 0.1%-NP-40 FBS (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The washed cell pellet was lysed in 5 pellet volumes of lysis buffer (50mM Tris pH 7.4, 50mM NaCl, 10% glycerol, 1.5mM MgCl2, 0.5mM CaCl2, 0.5% NP-40, 1x SigmaFast protease inhibitor [Sigma] ...
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bioRxiv - Bioengineering 2019Quote: ... PEGylated and unPEGylated polystyrene NPs were weighed and fully dissolved in a mixture of chloroform (CDCl3, Sigma), trifluoroacetic acid-d (TFAd ...
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bioRxiv - Cancer Biology 2020Quote: ... collected and lysed with NP-40 lysis buffer supplemented with phosphatase inhibitor cocktails 2 and 3 (Sigma) and protease inhibitor mix (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... PLGA) nanoparticles (NP) loaded with the protein (in the control condition bovine serum albumin, BSA, Sigma-Aldrich). The NPs were prepared according to the protocol described by Chaturvedi et al.29 ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.8) supplemented with 0.5 % NP-40 and protease inhibitor cocktail according to manufacturer’s recommendations (Sigma Aldrich) as described elsewhere82 ...
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bioRxiv - Genomics 2021Quote: ... 100 000 single cells were treated with 50 μl cold lysis buffer (0,1% NP-40 (492016, Millipore), 0,1% Tween-20 (P1379 ...
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Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: Cells were lysed with 1% NP-40 in phosphate-buffered saline (PBS) containing protease inhibitor cocktail (Sigma). After removal of nucleus by low-speed centrifugation ...
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bioRxiv - Immunology 2021Quote: ... NP-KLH was premixed in a 1:1 mixture of PBS: Complete Freund’s Adjuvant (CFA) (Sigma Aldrich) prior to injection ...
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bioRxiv - Cell Biology 2020Quote: ... NP-O) with a nominal spring constant of 0.06 Nm-1 were coated with ConA (Sigma, C7275)52 and applied for single cell adhesion as published47 ...
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bioRxiv - Immunology 2022Quote: ... DCs were pulsed with 20 µg/mL NP-CGG (LGC) and 100 ng/mL LPS (Sigma Aldrich) in R10 medium for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 1% NP-40) supplemented with 1 mM phenylmethyl sulfonyl fluoride (PMSF) and protease inhibitor tablet (Sigma, 11836170001). The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were collected and lysed in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, Sigma-Aldrich) and further processed for western blot analysis of the relative protein expression levels ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% NP-40) at 4°C for 2 h with gentle rotation in a siliconized microtube (Millipore). Afterwards 25 µl of Protein A/G magnetic beads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... NP and NPK mice were administered tamoxifen (Sigma; 10.8 mg/40 g body weight in corn oil) by oral gavage once daily for 1 day at the age of two months ...
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bioRxiv - Cell Biology 2023Quote: ... then thawed on ice and lysed in ice cold 200µl lysis buffer (1% NP-40 (Sigma Aldrich) in DPBS (Thermo Fischer Scientific)) ...
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bioRxiv - Genetics 2024Quote: ... were lysed with 175 μl of cold cytoRNA solution (50 mM Tris HCl pH 8.0; 140 mM NaCl; 1.5 mM MgCl2; 0,5% NP-40; 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5′ in ice ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 4-(4-Diethylaminostyryl-1-methyl-pyridinium-iodid (DiAsp) and NPS-2143 hydrochloride were purchased from Sigma-Aldrich and were kept at −20 °C at a stock concentration of 50 mM in DMSO.