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Citations for Millipore Sigma :
551 - 600 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... NPs were synthesized by utilizing copolymers fluorescently-labeled by the conjugation of fluorescein isothiocyanate (FITC, green fluorescence, Sigma-Aldrich) or rhodamine isothiocyanate (RITC ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were permeabilized by moving the coverslip to a drop of 0.25% NP-40 (IPEGAL CA-630, Sigma Aldrich) in PBS for 5 min RT.
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bioRxiv - Systems Biology 2022Quote: ... Cell pellets were then lysed in 0.1% NP-40 Tris-NaCl lysis supplemented with protease and phosphatase inhibitors (Sigma). Cell lysis was facilitated by three cycles of rapid freeze-thawing in liquid nitrogen ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in the same volume of Buffer I and an equivalent volume of ice-cold Buffer I supplemented with 0.2% NP-40 alternative (Sigma) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... cross-linked cells were first lysed 10min at 4°C in 500 µL of the Lysis Buffer 1 (10mM Tris pH8, 10mM NaCl, 0.5% NP-40, Complete protease inhibitor (Sigma)) then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8 ...
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bioRxiv - Cell Biology 2020Quote: HEK 293T cells or DRG sensory neurons were collected and prepped with lysis buffer (1% NP-40; 50mM Tris-HCl, pH 7.4; 150mM NaCl; 2mM EDTA; protease inhibitor (Sigma); and phosphatase inhibitor (Life Technologies)) ...
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bioRxiv - Biochemistry 2021Quote: ... An equal volume of NUN buffer (20mM HEPES pH 7.5, 10mM MgCl2, 300mM NaCl, 0.2mM EDTA, 1M urea, 1% NP-40, 1mM DTT, 1x SigmaFast Protease inhibitor [Sigma]) was gently added to the nuclear suspension and incubated on ice for 5 minutes ...
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bioRxiv - Genetics 2022Quote: ... followed by strong douncing in cell lysis buffer buffer (KCL 85 mM, HEPES 5 mM, NP-40 0.5%, Sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma) following by 5 min centrifugation at 2000g ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently scraped with ice cold RIPA buffer (50 mM Tris pH 8, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP-40; all chemicals from Sigma Aldrich) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl, 1 % NP-40, 0.5 % sodium deoxycholate, and 0.1 % SDS, supplemented with protease inhibitor cocktail; Sigma-Aldrich) for 30 min at 4°C and the protein content was measured by DC Protein Assay (Bio-Rad) ...
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bioRxiv - Developmental Biology 2019Quote: ... was mixed with 100 μl IP-buffer (50 mM Tris-HCL pH 7.5, 100 mM NaCl, 2 mM EDTA, 1% NP-40, 1x cOmplete EDTA-free protease inhibitor cocktails from Sigma) and antibody (for details on antibodies used see Table S3 ...
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bioRxiv - Genomics 2021Quote: ... The embryos were washed in embryo wash buffer (2 mM magnesium chloride [Ambion, catalog no. AM9530], 0.02% NP-40 substitute [Fluka, catalog no. 74385], 0.01% deoxycholate [Sigma-Aldrich, catalog no ...
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bioRxiv - Cancer Biology 2020Quote: ... were crosslinked in 1% formaldehyde for 7 min and lysed in cell lysis buffer (5 mM HEPES, 85 mM KCl, 0.5% NP-40, 1X protease inhibitor cocktail (Sigma), 1X phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... buffer 2 (20 mM Tris, 1 mM EDTA, 250 mM LiCl, 0.5% NP-40, 0.5% sodium deoxycholate, all from Sigma-Aldrich) three times and twice with TE buffer + 50 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... brachial LN were homogenized in 350 μl of tissue lysis buffer comprising of 0.5% NP-40 in PBS and protease inhibitor cocktail (Sigma), allowed to lyse at RT for 30 min ...
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bioRxiv - Cell Biology 2021Quote: 293T cells expressing GST-tagged constructs were lysed on ice in Pulldown Lysis buffer (150 mM NaCl, 50 mM Tris-Cl pH 7.4, 1% NP-40) supplemented with protease inhibitor (HALT, Sigma) cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... Cells close to confluency were washed with PBS and lysed with lysis buffer (1% NP-40 substitute (Sigma 74385) in PBS + protease inhibitors) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected in lysis buffer (150mM NaCl, 50mM Tris-HCl pH 8, 1mM DTT, 0.5% NP-40, with protease and phosphatase inhibitors (Sigma).After 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were resuspended and lysed in 500 µl of ice-cold PBS containing 0.1% NP-40 (Sigma-Aldrich). The whole cell fraction (100 µl ...
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bioRxiv - Cell Biology 2023Quote: ... respectively for the indicated point times and lysed with 100μl extraction buffer (50mM Tris pH=8.0, 150 mM NaCl, 0.5mM EDTA, 1% NP-40, and 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
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bioRxiv - Biochemistry 2023Quote: ... the nuclear lysate was obtained by resuspending the nuclear pellet in PBS containing 0.1% Nonidet P-40 (NP-40) and 1% protease inhibitor (#P8340, Sigma). Both the nuclear and cellular fractions were sonicated for 10 seconds of two cycles each with a holding period of 5seconds to cool down and then separated by SDS-PAGE and immunoblotted for TRF2 and TERF2IP ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and lysed in NP-40 buffer supplemented with 10 mM N-ethylmaleimide (Sigma, catalog no.: E3876). Flag-immunoprecipitations were performed as described previously ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Immunology 2024Quote: Cells were washed with ice-cold PBS and then lysed in 1% NP-40 lysis buffer (50mM Tris-HCI [pH 7.4], 150 mM NaCI, 1% IGEPAL CA-630 [Sigma]) supplemented with Halt Protease and phosphatase inhibitors for 15 min on ice (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by strong douncing in cell lysis buffer (KCL 85 mM, HEPES 5 mM, NP-40 0.5%, Sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma) following by 5 min centrifugation at 2000g ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed for 20 min in 1ml of lysis buffer (50mM Tris, pH=7.4, 150mM NaCl, 1mM EDTA, 0.5% NP 40) supplemented by 1 mM DTT (Sigma-Aldrich) and 1x protease inhibitors (Roche Applied Science) ...
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bioRxiv - Plant Biology 2024Quote: ... anti-FLAG magnetic beads were washed 5 times with cold wash buffer (GTEN buffer plus 0.1 % NP-40 (Sigma) and Roche protease inhibitor (50 ml/ 1 tablet)) ...
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bioRxiv - Neuroscience 2024Quote: ... In brief, brain tissues were homogenised in NP40 lysis buffer (1M HEPES, 5M NaCl, 0.1 EDTA, 1% NP-40 (Sigma): pH = 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed on ice using NP-40 or RIPA lysis buffer supplemented with protease inhibitors (Sigma-Aldrich, P8340). Cell lysates were then centrifuged at 14,000 rpm for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cells following subjected treatments were collected and lysed in NP40 buffer (50mM Tris-HCl pH 8, 150mM NaCl, 5mM EDTA, 0.5% NP-40, 1x EDTA-free protease inhibitor (Sigma-Aldrich), 1x phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore, Temecula ...
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bioRxiv - Genetics 2021Quote: ... Cleared lysates were adjusted to match the composition of the immunoprecipitation (IP) buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% NP-40 alternative (EMD-Millipore product 492018). The adjusted lysates were combined with anti-VSV-G agarose beads (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Crosslinked cells were resuspended in a pre-chilled lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.2% NP-40) with fresh protease inhibitors (Millipore Sigma). The lysate was then digested with the primary restriction enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended in KCl lysis buffer (50 mM Tris-HCl pH 7.5, 225 mM KCl, 1% NP-40) with a protease inhibitor cocktail (Sigma-Aldrich) and other inhibitors (1 mM PMSF ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Neuroscience 2021Quote: ... The lysates were cleared by centrifuging at 2000 xg for 10 min at 4°C and treated with DHPC (to 30 mM, Avanti) and NP-40 (to 1%, Ipgal-ca630, Sigma) for 5 min in ice ...
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bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
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bioRxiv - Cell Biology 2021Quote: CCRF-CEM cells were collected and lysed in lysis buffer (PBS, 0.2% NP-40, 1 mM EDTA, protease inhibitor cocktail (Sigma-Aldrich) and 2 mM PMSF) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 225 mM KCl, 1% NP-40) with a protease inhibitor cocktail (Sigma-Aldrich) and other inhibitors (1 mM PMSF ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Microbiology 2022Quote: ... diluted with 600 µL of dilution buffer (lysis buffer without NP-40) and incubated with 25 µL of pre-washed Anti-FLAG M2 Magnetic Beads (Sigma) on a rotor overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... then scraped into 3 ml ice cold lysis buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40 substitute (Sigma 74385), 1x Protease inhibitor (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... after transfection and functional analysis using NP-40 lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris-HCl pH7.6) supplemented with 1× protease inhibitor cocktail (Sigma-Aldrich). Protein concentration was measured using a Coomassie (Bradford ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Sigma Millipore) was added to the thawed pellet ...
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bioRxiv - Genomics 2019Quote: ... sorted samples were re-suspended in 1ml Cell Lysis Buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40/Igepal) containing fresh protease inhibitors (Sigma P8340) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Physiology 2019Quote: ... 50 mM Tris-HCl pH 7.4, 1% NP-40, 0.1% sodium deoxycholate, 1 mM EDTA, all from Sigma-Aldrich, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed by scraping them into a pH 7.4 lysis buffer containing 1% NP-40 (Sigma-Aldrich, Gillingham, UK), 50 mmol/L Tris ...