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Citations for Millipore Sigma :
501 - 550 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and lysed in 0.1% NP-40 with phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). After brief sonication ...
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bioRxiv - Physiology 2020Quote: Cells in suspension were washed with PBS and lysed using NP-40 lysis buffer containing proteinase inhibitors (Sigma) and phosphatase inhibitors (Sigma ...
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bioRxiv - Biochemistry 2021Quote: Cells expressing GRAMD1C-EGFP or GRAMD1C(ΔGRAM)-EGFP were lysed with NP-40 lysis buffer (150 mM, 1.0% NP-40, 50 mM Tris-HCl pH 8.0) supplemented with PhosStop phosphatase inhibitor (Sigma) and complete protease inhibitor cocktail (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... Lysed worm powder was thawed on ice in a final volume of 3.75 ml lysis buffer including 0.5 % v/v NP-40 (Sigma), 0.4 U/μL RNasin (Promega) ...
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bioRxiv - Pathology 2021Quote: ... was homogenized with protein lysis buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP-40, 0.5mM PMSF(Sigma) and cOmplete protease inhibitor (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated at 4°C in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.1% SDS, Sigma protease inhibitors 1:500 ...
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bioRxiv - Immunology 2020Quote: Whole Cell lysates were prepared with the RIPA buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1mM EDTA with protease inhibitor cocktail [Sigma]), isolated from uninfected ...
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bioRxiv - Immunology 2020Quote: Cells were lysed in RIPA buffer (50 mM Tris.HCl, pH7.4; 150 mM NaCl; 1% NP-40 (Sigma-Aldrich), 0.5% Deoxycholate ...
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma) for 15 minutes at 4°C and centrifuged for 10 minutes at 300xg at 4°C to obtain cell lysates ...
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bioRxiv - Bioengineering 2023Quote: ... NPs were purified by centrifugation over a filter (molecular weight cutoff of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg mL−1 ...
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bioRxiv - Bioengineering 2023Quote: ... NPs were purified by centrifugation over a filter (molecular weight cutoff of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... All cells were lysed in RIPA-buffer (50 mM Tris-HCl pH 8.0, 1 % NP-40 (Sigma, 492018), 0.5 % sodium deoxycholate (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting supernatant or ‘secondary virus’ was filtered using 0.45 μm pore size sterile filter (Millipore) and was used immediately to infect large-scale expression cultures ...
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bioRxiv - Cell Biology 2022Quote: ... the medium containing the virus particles was recovered and concentrated with Polyethylene Glycol 8000 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were infected with serial dilutions of virus diluted in 1× minimum essential medium (MEM, Sigma) supplemented with 0.6% BSA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... HeLa cells were incubated overnight in growing media plus virus and polybrene (Sigma-Aldrich, TR-1003). The following day ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG, Sigma # P4338). V6.5 cells were infected in medium containing 5ug/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: Virus propagation was performed in Vero E6 cells in DMEM (Sigma-Aldrich, St Louis, MO, USA) supplemented with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... the virus containing suspension from HEK293T cells was filtered with a 0.45 μM filter (EMD Millipore), supplemented with polybrene (4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Virus was added at the indicated titer in the presence of polybrene (2µg/mL, Sigma, H9268) and the cells were incubated for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and the pellets were directly lysed in 750 µl of Polysome Lysis Buffer (10mM HEPES pH 7.4, 150mM KCl, 5mM MgCl2, 0.5% NP-40, 0.5mM DTT (Sigma-Aldrich, D0632), 50mM iodoacetamide ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore ...
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bioRxiv - Developmental Biology 2021Quote: Whole embryonic mouse brains were harvested at E15.5 and lysed in NP-40 lysis buffer with protease inhibitor cocktail (Sigma). Whole brain lysates from one embryo were added to either 6μg GST or 6μg GST-MLLT11 bead slurries and nutated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells’ membranes was lysed by adding 1 volume of the 2x Lysis Buffer (Buffer N supplemented with 0.6% NP-40 substitute (Sigma)) to the single cell suspension resuspended in 2 PCVs (packed cell volumes ...
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bioRxiv - Developmental Biology 2022Quote: ... lysed them in NP40 lysis buffer (50mMTris-HCl, 150mMNaCl, 0.1% NP-40, 5mM EDTA, 10% glycerol) with protease inhibitors cocktail (Sigma), and sonicated using a Bioruptor (Diagenode) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 2 mM MgCl2 and protease inhibitor cocktail [Sigma]). 10% of lysate volume was reserved to determine total protein amounts ...
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bioRxiv - Molecular Biology 2021Quote: ... then they were resuspended in cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Sigma-Aldrich) complemented with 1xPIC (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Bioengineering 2022Quote: GelMA and gelMA-Au NP prepolymer solutions comprising 10 or 20% w/v gelMA and up to 37 mM Au NPs were supplemented with 0.5-1.0% w/v Irgacure 2959 (Sigma-Aldrich) or lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed on ice in 5 ml lysis buffer (10 mM Tris-HCl, pH 8, 10 mM NaCl, 0.2% Igepal NP-40 (Sigma), 1× cOmplete protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were permeabilized in 1ml lysis buffer (10mM Tris–HCl pH 8, 10mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Sigma) for 15 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... ~2×108 cells were collected and lysed in a hypotonic buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Sigma) for 20 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by three washes in low-salt buffer (20 mM HEPES KOH, pH 7.4, 10 mM MgCl2, 150 mM KCl, 1% NP-40 [Sigma I8896-50ML] ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed with modified RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA, 1% NP-40, 0.25% deoxycholic acid; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors ...
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bioRxiv - Bioengineering 2021Quote: ... NPs were purified by ultracentrifugation over a filter (molecular weight cut-off of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in water to a final concentration of 250 mg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... NPs were purified by ultracentrifugation over a filter (molecular weight cut-off of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in water to a final concentration of 200 mg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... followed by total protein isolation with extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% [v/v] NP-40 and 1:200 protease inhibitor cocktail from Sigma). Co-IP was carried out the same as for co-IP with protoplasts except that GFP-Trap agarose beads (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...