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Citations for Millipore Sigma :
701 - 750 of 886 citations for Ebola Virus Like Particles since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The supernatant was then passed through a 0.45 µm filter to remove debris and virus was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Sigma, UFC9100). For this ...
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bioRxiv - Cancer Biology 2019Quote: ... Functional titration was performed by transduction of cells with serially diluted virus in the presence of polybrene (4 μg/ml, Sigma) for 6 hours followed by puromycin (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
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bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and the virus containing supernatant was loaded on a previously with lysis buffer pre-equilibrated 1 ml HiTrap Heparin HP column (SIGMA). Several washing steps later (increasing salt conditions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Microbiology 2019Quote: ... of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro], 25 mM HEPES [Corning cellgro], 0.3% BSA [Sigma-Aldrich]) with 0.25 μg/ml TPCK-trypsin ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Influenza viruses as well as vesicular stomatitis virus strain Indiana (VSV) were propagated on Madin-Darby canine kidney (MDCKII) cells cultured in minimal essential medium (MEM-Sigma) containing 10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Virus transduction capability was then tittered on 293T-Ace2 cells treated with 50μl of 5μg/ml polybrene (Sigma-Aldritch LLC). LzGreen titer was determined by fluorescence using BZ-X700 all-in-one fluorescent microscope (Keyence) ...
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bioRxiv - Immunology 2021Quote: ... Dengue virus was added at a MOI=1 in a minimum volume of Dubelcco’s PBS with CaCl2 and antibiotics (SIGMA-Aldrich). After 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cells were resuspended at a concentration of 106 cells/mL in virus-containing medium supplemented with 10 μg/mL polybrene (Sigma), divided into 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Immunology 2022Quote: ... hTert-BJ1 or U937 were infected with 2 ml of fresh virus in presence of 8µg/ml Protamine (Millipore Sigma). To generate transduced MDDCs ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and the virus was resuspended in ultrapure water containing 0.005% Bovine serum albumin (Sigma-Aldrich, USA). Purified and filtrated SARS-CoV-2 pseudovirus was firstly fixed in 2% glutaraldehyde (Leagene ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were infected with either of the sgPLXNB2 clones or the control sgRNA with Cas9-GFP virus (Sigma-Aldrich #0030) at 10 IFU/cell in Opti-MEM (Thermo Fisher Scientific #31985070 ...
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bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were transduced with equal titers of concentrated virus in complete growth media supplemented with 1 μg/ml polybrene (Sigma) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 786-O cells were cultured with virus containing media and 4µg/mL polybrene (Sigma Aldrich, Cat. No. TR-1003-G) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... The next day 50 µl of concentrated virus was added together with 5 µg/ml polybrene (Millipore, TR-1003-G) to the culture media ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Plant Biology 2019Quote: ... A ratio of 1:2 and 1:3 (v/v) polyvinylpolypyrrolidone (PVPP, ~110 µm particle size, Sigma-Aldrich, Saint Louis, USA) to ground tissue was used ...
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bioRxiv - Cell Biology 2020Quote: ... or human NUP153-specific shRNA lentivirus particles overnight at 37°C followed by selection in medium containing Puromycin (Puro, 2 μg/ml) (Sigma-Aldrich) for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: Lentiviral particles were either generated by the laboratory (NF-κB or p53 reporter) or obtained from the MISSION shRNA library (Sigma-Aldrich). These obtained lentiviral particles harbor the following shRNA clones in pLKO.1 backbone vector and were used to infect BJ cells ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... hESC and HT-29 cells were infected by lentiviral particles incubated in the growth medium containing and 8μg/ml Polybren (Sigma, 107689-10G) to attached cells ...
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bioRxiv - Bioengineering 2019Quote: ... Inks consisted of particles suspended in 1% alginate or pristine alginate (CAS 9005-38-3, Sigma Aldrich, St. Louis, MO, USA) solutions.
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bioRxiv - Cancer Biology 2019Quote: ... Supernatants containing lentiviral particles were used to infect MDA-LM2 cancer cells overnight in the presence of 8 μg/ml polybrene (Sigma-Aldrich). Infected cells were selected with 2 μg/ml puromycin (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... were 55 μm pre-filtered to remove large particles and then 0.22 μm filtered (cat. GPWP14250 142 mm Express Plus Filter, Millipore; Darmstadt, Germany) using a peristaltic pump ...
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bioRxiv - Cell Biology 2021Quote: ... hCMEC/D3 cells were transduced first by lentiviral particles expressing Cas9 and blue fluorescent protein (BFP) as a transduction marker (LVCAS9BST, Sigma-Aldrich). Cas9-expressing cells were then selected with blasticidin antibiotic at 50 μg/mL for 6 days (Life technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... RMS cells were infected with viral particles for 24 hr at 37°C using 8 mg/mL of polybrene (EMD Millipore).
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bioRxiv - Immunology 2022Quote: ... or HKLM (TLR2 agonist, 108 particles/ml) for 8 hours in the presence of 5 μg/ml brefeldin A (Sigma-Aldrich). TLR agonists were purchased from InvivoGen (San Diego ...
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bioRxiv - Immunology 2022Quote: ... the gradient purified viral particles were polished by size-exclusion chromatography using the HiPrep 16/60 Sephacryl S-500 HR column (Sigma Aldrich) running in TBS buffer (Alfa Aesar) ...
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bioRxiv - Immunology 2022Quote: ... Viral particles pseudotyped with RD114 were generated using 293T cells with GeneJuice® transfection reagent (Sigma-Aldrich, Saint Louis, MO, USA) following manufacturer’s instructions with a 1.5:1.5:1 μg ratio of the SFG:Peq-Pam:RD114 DNA plasmids and concentrated using Retro-X™ Concentrator (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... the 22RV1 and LNCaP cells were infected with the collected lentiviral particles along with polybrene (hexadimethrine bromide; 8 µg/ml) (Sigma-Aldrich). Culture media was changed the next day and puromycin (Sigma-Aldrich ...