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Citations for Millipore Sigma :
451 - 500 of 886 citations for Ebola Virus Like Particles since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: Virus carrying the gene of interest was used to infect cell lines with 10mg/mL polybrene (Millipore). Media was replaced after 24 h and cells were sorted for GFP positivity after 48-72 h post-infection ...
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bioRxiv - Genetics 2019Quote: ... The resulting myoblasts were then infected with a retroviral cocktail containing Myf6-CTAP virus and polybrene (Millipore) for 8 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Suspensions of L-cells (from ATCC) for virus production were maintained in Joklik MEM medium (Sigma-Aldrich) supplemented with 1% L-Glutamine (Gibco.) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore) filter ...
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles were used to construct stable cell lines knocked down against mismatch control sequence (shMM, Sigma SHC002) or target genes ...
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bioRxiv - Immunology 2019Quote: ... Viral particles were added to cultured THP-1 cells in R10 together with 8 μg/ml Polybrene (Sigma) to improve transfection efficiency ...
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bioRxiv - Cell Biology 2020Quote: ... dust particles and aggregates of casein in the buffer were removed using a 100 kD centrifugal filter (Millipore). Another potential issue is the stage drift during measurement and it was minimized with an automated drift correction system using a xyz piezo stage (PI ...
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bioRxiv - Cancer Biology 2020Quote: ... the media with viral particles was collected and syringe filtered through a 0.45 µM filter (Millipore, Burlington, MA). The filtered viral supernatant was used immediately to transduce target cells (PDACc/PANC-1 or fibroblasts ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: shRNA Endo180 knock-down clones from 3T3 and CAFs were generated using lentiviral particles (Sigma, Supplementary Table 3). For siRNA knockdown ...
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bioRxiv - Bioengineering 2021Quote: ... the particles were suspended in 1x PBS containing glutaraldehyde (GA) at 2.5□wt% (Sigma-Aldrich cat. no. G6257) and kept mixing at room temperature for 3□h ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were infected with 100μl of lentiviral particles suspension along with 10 μg/ml polybrene (Sigma-aldrich,USA) 1.5ml of fresh DMEM media containing 10% FBS without antibiotics and incubated for minimum of 24hrs ...
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bioRxiv - Genetics 2021Quote: ... Polar metabolites were separated on a ZIC-pHILIC 150 × 2.1 mm (5-μm particle size) column (EMD Millipore) connected to a Thermo Vanquish ultrahigh-pressure liquid chromatography (UPLC ...
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bioRxiv - Cell Biology 2021Quote: ... Adherent HEMs or MNT1 cells were incubated with viral particles and 8 μg/ml polybrene (H9268, Sigma-Aldrich) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 cells were infected with Lentiviral packaged particles by supplementing 8 μg/ml protamine sulfate (P4505; Sigma-Aldrich), the virus infected cells were selected by overnight puromycin (7 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... calcium-phosphate-DNA-particle were generated by mixing the plasmid DNA’s with a 250 mM CaCl (Sigma-Aldrich) solution which was then added dropwise to an equal volume of 2 x hepes buffered saline and subsequently incubated for 20 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Pseudotyped particles were concentrated from producer cell lysates that were overlaid a 10% OptiPrep cushion in PBS (Sigma) and centrifuged at 20,000× g for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... The mouse MSCs were infected with the lentiviral particles by the addition of hexadimethrine bromide (Sigma-Aldrich, USA). Lentivirus infected mouse MSCs were selected by G418 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... and the number of viral particles (PFUs) visualized after staining with 1% (w/v) crystal violet (Sigma-Aldrich). Controls were the viral inoculum incubated in DMEM or PBS without the antimicrobial gel ...
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bioRxiv - Biochemistry 2019Quote: Freshly prepared samples were loaded on a SeQuant ZIC-HILIC capillary column (0.3 mm × 150 mm, 3.5 μm particle size, 200 A pore size, Millipore) using the capillary pump of a Dionex Ultimate 3000 Nano LC system with autosampler (Dionex ...
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bioRxiv - Genomics 2021Quote: ... The AAV particles were precipitated with Polyethylene Glycol (PEG 8000, Sigma-Aldrich, St. Louis, MO; cat. P2139-500G) and purified on an iodixanol gradient (OptiPrep ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Microbiology 2020Quote: ... a small batch of VSVΔG-rLuc particles bearing the CoV2pp were then treated with TPCK-treated trypsin (Sigma) at room temperature for 15 minutes prior to inhibition with soybean trypsin inhibitor (SBTI) ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant containing the viral particles was added to target cells along with 10 ng/ml Polybrene (Millipore) for 24 h ...
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bioRxiv - Immunology 2022Quote: ... The cell supernatants containing lentiviral particles were harvested after 48 h and filtered using 0.45 μm filter (Millipore). pCDH-lentivirus were used to transfect CHO cells with 8 μg/ml polybrene to express FcγRI (pCDH::Fcgr1) ...
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bioRxiv - Microbiology 2022Quote: ... They were then subjected to bead beating (3 × 45 s) with glass beads (particle size ≤ 106 μm; Sigma) using a MP Biomedicals™ FastPrep-24™ (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... MSK-QRESS-KIT] and 1μL was injected into a ZIC-pHILIC 150 × 2.1mm (5μm particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... flavefaciens was adapted to growth on microcrystalline cellulose (Avicel PH-101, 50 μm particle size, Sigma-Aldrich, UK) as the main carbon source ...
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bioRxiv - Microbiology 2023Quote: ... These were initially added 0.2 g sterilized Ottawa sand (50–70 mesh particle size, Sigma-Aldrich, Taufkirchen, Germany) each ...
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bioRxiv - Biochemistry 2023Quote: ... HEK 293t cells were infected with commercially available Lentiviral particles for hTRMT2A KD and scrambled control cell line (MISSION® shRNA Lentviral Transduction Particles: scrambled control WT = SHC002V, KD1 = NM_182984.2-856s1c1, KD2 = NM_182984.2-1574s1c1; Sigma-Aldrich). Cells with stable integration of shRNA were selected as puromycin-resistant colonies ...
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bioRxiv - Cell Biology 2023Quote: Gene editing of Tecpr1 in MEFs was achieved using custom CRISPR gRNA lentivirus transduction particles (Mission, Sigma Aldrich). MEFs were infected with the CRISPR gRNA lentiviruses in Opti-Minimal Essential Medium (Opti-MEM ...
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bioRxiv - Bioengineering 2023Quote: ... whereas the viral particles present in the supernatant were concentrated with 8% PEG8000 (Polyethylene glycol 8000, Sigma-Aldrich) by precipitation (4000g ...