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Citations for Millipore Sigma :
551 - 600 of 662 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 1 hour) and Yta7-FLAG3x and mutant versions were bound to pre-washed anti-FLAG M2 affinity gel (Sigma) in batch for 45 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: Transgenic or mutant embryos were grown to the 10 somite stage and then transferred to Modified Barth’s Saline (MBS) (Sigma). In this medium ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: E14 mouse embryonic stem cells (E14Tg2a.4) (RRID:MMRRC_015890-UCD) from Mutant Mouse Regional Resource Centers were maintained on 0.1% gelatin (Sigma-Aldrich G1393) coated 6-well plates under the serum/LIF condition containing 15% ES-grade FBS (Gibco 16141061) ...
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bioRxiv - Developmental Biology 2023Quote: Control and mutant embryonic cortices from E13.5 animals were dissected and dissociated into single cell suspension and digested with Acutase (Sigma). Cells were maintained in proliferation media (STEMCELL Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... npq4 mutant (when indicated) was grown in TAP medium supplemented with different concentrations of PEG - 8000 (Sigma - Aldrich, Germany). Under continuous illumination (50 μmol photons m-2 s-1 ...
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bioRxiv - Biophysics 2023Quote: ... MCM9_1-276) and relative mutants were cloned into multiple cloning site I (MCS I) of pRSFDuet-1 vector (Novagen). All of the mutations were introduced by the standard PCR-based mutagenesis method described before (42) ...
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bioRxiv - Biochemistry 2023Quote: Genes encoding VanX (C78/157S mutants with and without W24R) were constructed using the pET-47b plasmid vector (Novagen). The HRV3C recognition sequence and a linker to improve the digestion efficiency were inserted between the (His)6-tag and the vanx sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... Transient transfection with mutant MLH1 constructs (purchased from TWIST Biosciences) and siRNA against CDK4/6 (purchased from Sigma-Aldrich) was conducted using JetPrime PolyPlus transfection reagent ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Crude bacterial template DNA for toxin profiling was prepared by resuspension of cells in a 5% (wt/vol) solution of Chelex-100 resin (Sigma-Aldrich, Castle Hill, NSW, Australia). All isolates were screened by PCR for the presence of toxin A (tcdA) ...
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bioRxiv - Cell Biology 2020Quote: CHO-K1 cells expressing human α1ATWT or the polymerogenic α1ATH334D mutant under a tetracycline inducible promoter (Ordonez et al., 2013) were cultured in DMEM cell media (Sigma) supplemented with 10% Tet-free serum (Pan-Biotech) ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... All deletion and point mutants were generated by Quick change site-directed mutagenesis protocol using KOD Hot Start Polymerase (Millipore) and DpnI (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... of wild-type Rad6 or an A126 mutant was PCR amplified and inserted by SLIC into BamH1-Not1 sites in bacterial expression vector pRSF-Duet (Novagen). Subsequently ...
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bioRxiv - Neuroscience 2019Quote: ... 5dpf WT and rnaset2sh532 mutant zebrafish larvae were anaesthetised and imbedded in low melting point agarose containing tricaine (0.168 mg/ml; Sigma-Aldrich) and imaged using a 40x objective on a UltraVIEW VoX spinning disk confocal microscope (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Plant Biology 2019Quote: ... the roots of WT and ELF4-ox (fused to GFP) grafted shoots into elf4-1 mutants were placed on microscope slides (Sigma). Fluorescent signals were imaged with an argon laser (transmissivity ...
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Sterol flow between the plasma membrane and the endosome is regulated by the LAM family protein Ltc1bioRxiv - Cell Biology 2019Quote: Imaging of sec8 mutant and FM4-64 uptake experiments were done in glass chambers coated with soybean lectin (Glycine max lectin, Sigma). Shortly chambers of 96-well-plates (MGB096-1-2LG-L ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2020Quote: The human wt-PD-1 (33 – 150 aa, C93S mutation) and mutants (N66A, Y68A, E135A and N66AY68A) were cloned into pET24d (Novagen) while recombinant PD-L1 (18 – 134 aa ...
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bioRxiv - Plant Biology 2020Quote: ... The coding sequence of PhS3-RNase (without signal peptide) as well as its six mutant forms described above were separately cloned into engineered pET-30a (Novagen) containing N-terminal SUMO tag to produce SUMO-His-tagged proteins ...
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bioRxiv - Plant Biology 2020Quote: The coding sequences of PhS3-RNase (without signal peptide) and its six mutant forms were separately cloned into pMAl-c2x (Novagen) to generate MBP-tagged fusion proteins ...
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bioRxiv - Microbiology 2021Quote: ... coli O111:B4 (catalog no. L3024) and lipid A from E.coli F583 (Rd mutant, catalog no. L5399) were purchased from Sigma Aldrich. Deuterium oxide was from Cambridge Isotope Laboratories (Tewksbury ...
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bioRxiv - Neuroscience 2020Quote: ... in the WT and F147L/F149L mutant forms were subcloned from the pCS2 vector (Kabashi et al., 2010) to the pET21b vector (Novagen) modified to include a TEV cleavage site ...
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bioRxiv - Neuroscience 2022Quote: ... Tamoxifen induction of Cre recombination in the double mutant embryos was performed by gavage administration of tamoxifen (5 mg dissolved in corn oil, Sigma) at E10.5 to specifically target the principal sensory thalamic nucleus ...
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bioRxiv - Evolutionary Biology 2022Quote: The ΔscDH mutant was concentrated at 10 mg/ml in SEC buffer using a 30kDa molecular weight cutoff spin concentrator (Millipore). SAH was added at 1 mM to the concentrated protein sample ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... aureus Cowan I wildtype as well as the isogenic mutants ΔpknB and Δstp were grown to logarithmic phase and 108 CFUs mixed 1:1 with cytodex beads (Sigma) 60 ...
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bioRxiv - Microbiology 2020Quote: ... validated as hex1 deletion mutants by spore PCR and Southern blot analysis (DIG DNA Labeling and Detection Kit, Sigma-Aldrich), and stored as 25% glycerol stocks at −80 °C.
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bioRxiv - Microbiology 2021Quote: T1L WT or T1L σ3 mutant virions (2×1012 particles per ml in 100 μl) were incubated with 10 μg/ml trypsin (Sigma) at 8°C in a MyCyclerTM thermal cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... mt1 and mt2 were generated by inserting the ORF of human TruB1 and its mutants into the pET22b vector (Merck Millipore) at the NdeI and XhoI sites ...
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bioRxiv - Microbiology 2022Quote: ... NIH clone A2 and the isogenic Δgp63 mutants and their complemented counterparts Δgp63+GP63 (13) were cultured at 26° C in Leishmania medium (M199 [Sigma] supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2022Quote: ... Ksp-cre transgenic mice in both Nnt mutant or wild type backgrounds was constantly treated with N-acetyl-L-cysteine (NAC; A7250 Sigma) mixed in drinking water at 7 mg/mL 26 supplemented with 5 mg/ml of Splenda sweetener ...
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bioRxiv - Developmental Biology 2022Quote: ... and wild-type or dominant-negative GJA1 and Rab11a mutants were detected using anti-Flag M2 magnetic beads (Sigma Aldrich), which captured the GJA1 Flag-tagged proteins ...
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bioRxiv - Neuroscience 2019Quote: ... pigmentless (nac−/−) Dscaml1 heterozygous and homozygous mutant siblings were immobilized in a gel of 1.8% low-melting temperature agarose (Sigma-Aldrich) in preparation for imaging ...
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bioRxiv - Microbiology 2021Quote: ... S2 or S2 P-mutant subunits in the presence of increasing doses (0.25-1 ng/ml) of LPS (Sigma, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 mg/ml of purified PIM1 and PIM1 mutants were incubated at 37 °C with or without 0.08 mg/ml beta-casein (Sigma-Aldrich). Aliquots were taken at specified time intervals and immediately mixed with 6X SDS sample buffer to terminate the reaction ...
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bioRxiv - Genetics 2020Quote: Paromomycin sensitivity was evaluated at 27°C by following over nine days the growth of four subcultures of wild-type and mutant mat+ strains on M2 medium supplemented (or not) with 500 μg ml-1 of paromomycin (Sigma). For each strain ...
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bioRxiv - Biochemistry 2022Quote: Streptococcus pyogenes Cas9 wild type protein and the nuclease dead mutant (D10A, H840A) were both recombinantly expressed for 16 hours at 18 °C in Escherichia coli Rosetta 2 (DE3) (Novagen) N-terminally fused to a hexahistidine affinity tag ...
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bioRxiv - Microbiology 2022Quote: ... the wild-type H99 and isw1Δ mutant strains were either untreated or were treated with 10 μg/ml FLC (Sigma) in 50 ml YPD media at 30°C until cell densities reached the exponential phase (approximately 6 to 7 hours) ...
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bioRxiv - Plant Biology 2023Quote: ... fresh sporangia produced after crossing T0 Mprop mutants to wild-type (Tak-1 or Tak-2) were sterilised in 1 % (w/v) sodium dichloroisocyanurate (Sigma) solution for 3 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... encoding the human KRASG12V and KRASY64A mutants (residues 1–169) and human SOS1 (residues 564–1049) were cloned as NdeI and XhoI fragments into pET28b (Novagen). His-tagged KRAS•GDP (abbreviated RAS ...
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bioRxiv - Cancer Biology 2023Quote: ... age-matched mutant mice together with wild-type negative control C57BL/6J mice were injected intraperitoneally with tamoxifen (Sigma, T5648) in corn oil at a concentration of 2 mg per 20g body weight at age 8 – 12 weeks ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Unique integration of the cassette in the mutants was then controlled by Southern blot using DIG-High Prime DNA Labelling and Detection Starter Kit I (Sigma). Primers used for these experiments are listed in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines stably expressing the WT/mutant proteins were selected using media supplemented with 5 μg/ml puromycin (Sigma-Aldrich). The reconstitution of mutant GSECs was assessed by SDS-PAGE/western blotting ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... acr-2(gf) double mutants were washed into a 2 mL solution of M9 media with 47 mM ethyl methanesulfonate (Sigma) and incubated at 22°C on a spinning wheel for 4 hours ...
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bioRxiv - Genetics 2024Quote: ... Approximately 40 L4 stage mutant animals were then picked and placed on 35 mm OP50-seeded NGM plates containing 0.5 mM aldicarb (Sigma Aldrich, 33386 ...