Labshake search
Citations for Millipore Sigma :
451 - 500 of 662 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... cells were incubated with the following mitochondrial toxins for 16 h: 1.25 ng/mL oligomycin (Sigma-Aldrich # 75351), 50 µg/mL doxycycline (Clontech # 631311) ...
-
bioRxiv - Cancer Biology 2019Quote: ... FNE1/FNE2 cell lines were maintained in FOMI media (University of Miami) supplemented with cholera toxin (C8052, Sigma). H2B-RFP stable cell lines were generated after transfection with lentiviral construct H2B-RFP (Addgene 26001 ...
-
bioRxiv - Immunology 2021Quote: Formyl-methionyl-leucyl-phenylalanine or fMLF (F3506), DMSO, and Pertussis toxin (PT, P7208) were purchased from Sigma-Aldrich. Human recombinant CXCL8 (CHM349) ...
-
Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... CHO cells were incubated with 6E10 and 10 μg/ml FITC-conjugated cholera toxin B subunits (Sigma, #C1655) to label lipid raft microdomains at 4°C for 1 h before fixation ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20 ng/ml basic fibroblast growth factor (bFGF, R&D) and 50 ng/ml Cholera Toxin (Merck-SIGMA). Cells were counted using a haemocytometer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... we used these toxins to conjugate to NHS-activated Sepharose® 4 Fast Flow beads (Sigma-Aldrich, UK) to generate ssDNA aptamers as novel toxin inhibiting therapeutics ...
-
bioRxiv - Immunology 2023Quote: ... The immunized mice also received intraperitoneal (i.p.) injections of 200 ng of Bordetella pertussis toxin (PTx) (Sigma-Aldrich) at the time of each immunization and 48 h later ...
-
bioRxiv - Immunology 2023Quote: CD11c-DTR or control C57Bl/6J mice were injected intraperitoneally with 5ng/g diptheria toxin (Sigma-Aldrich, D0643) every 3 days ...
-
bioRxiv - Biophysics 2024Quote: ... 10 mM creatine phosphate in IB) and 50 μg/ml of Staphylococcus aureus α-toxin (Hemolysin, Sigma-Aldrich) to permeabilize the bleb membrane and allow for ATP and UTP to penetrate the blebs ...
-
bioRxiv - Neuroscience 2020Quote: ... Both immunized and SHAM mice were administered with Pertussis toxin (400 ng/mouse, Sigma-Aldrich, St. Louis, MO, USA), injected intravenously through the tail vein on the day of immunization and 48 h later ...
-
bioRxiv - Microbiology 2022Quote: ... BMDMs were pre-treated with the following inhibitors or vehicle controls: pertussis toxin (Sigma, 1 μg/mL 2 h), SB290157 (Sigma ...
-
bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
-
bioRxiv - Cell Biology 2023Quote: ... retinoic acid and epidermal growth factor (EGF) as per the SAGM Bullet Kit and 0.1 μg ml−1 cholera toxin (cat. no. C8052, Sigma), 5% FBS and 1% Pen/Strep ...
-
bioRxiv - Neuroscience 2019Quote: ... The wild-type or T94M mutant ORFs were then PCR-cloned into pET28c (Novagen) for bacterial production of the proteins with an N-terminal 6× His tag ...
-
bioRxiv - Plant Biology 2020Quote: MBP tagged SyYVCV βC1 and mutants were transformed in Rosetta Gammi DE3 cells (Novagen) according to manufacturer’s directions ...
-
bioRxiv - Biophysics 2020Quote: ... except comparison of TSG101 mutants proceeded by 6-well plates (9.4 cm2 wells, Sigma). The NEB Gaussia and Cypridina plasmids were used at 40 ng each per well for each 96-well assay (or ten times as much for 6-well plates) ...
-
bioRxiv - Biochemistry 2021Quote: ... smegmatis mc2 155 strain and its mutants were propagated in liquid 7H9 medium (Sigma) with 10% ADC supplement (5% BSA ...
-
bioRxiv - Developmental Biology 2022Quote: The wild-type and stat3 mutant zebrafish was euthanized with tricaine methanesulfonate (MS222, Sigma) and fixed using 2.5% glutaraldehyde (diluted in PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... wild-type and mutant NSCs were cultured and treated with or without BMP4 (Sigma). After 48-hour treatment ...
-
bioRxiv - Biochemistry 2023Quote: ... Wild-type HROB and mutants were purified using FLAG M2 Affinity Gel (Sigma, A2220) based column chromatography39.
-
bioRxiv - Cell Biology 2023Quote: ... EqtII mutants were generated by site-directed mutagenesis and then introduced into pET45b (+) (Novagen) for bacterial protein expression and pRetroX-TetOne-puro (clontech ...
-
bioRxiv - Neuroscience 2023Quote: ... eGFP and PAK1 mutant expression were induced with doxycycline (2 μg/mL, Sigma-Aldrich) at 1DIV post-transduction ...
-
bioRxiv - Biochemistry 2024Quote: ... Binding pocket mutants were generated by site-directed mutagenesis using primers from Millipore sigma and sequenced (Elim Biopharmaceuticals ...
-
bioRxiv - Cell Biology 2019Quote: MCF10A cells were grown as previously described (Debnath et al.) in DMEM media (Biological Industries) supplemented with 100 ng/ml cholera toxin (Sigma), 20 ng/ml epidermal growth factor (EGF ...
-
bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
-
bioRxiv - Neuroscience 2022Quote: ... selective astrocytic toxin L-α-aminoadipate (L-AA) as well as Gabapentin were purchased from Sigma-Aldrich (St. Louis, MO). Caspase-1 siRNA was purchased from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were treated with anthrax toxin (0.5 µg/mL PA and 0.1 µg/mL LF) in minimal media (Glasgow minimal essential media (Sigma-Aldrich G6148) buffered with 10 mM HEPES ...
-
bioRxiv - Microbiology 2022Quote: Total protein was extracted from LT toxin-treated (100ng/ml) human differentiated enteroid monolayers (235D line) and untreated control using radioimmunoprecipitation assay (RIPA) buffer (Sigma) containing protease inhibitor cocktail ...
-
bioRxiv - Neuroscience 2022Quote: Retrograde labelling of preBötC→7n neurons was achieved by either the injection of AAVrg-CAG-GFP (1.29x1013VP/ml) or Alexa Fluor 647-conjugated cholera toxin subunit B (CTB; Sigma, C34778) into 7n ...
-
bioRxiv - Immunology 2023Quote: ... On the day of immunization as well as 2 days later mice were injected intraperitoneally (i.p.) with 400 ng pertussis toxin (Sigma Aldrich). Disease progression in mice was monitored daily and assessed according to the following disease scores ...
-
bioRxiv - Microbiology 2023Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
-
bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
-
bioRxiv - Genetics 2020Quote: ... we anaesthetized embryos from incrosses of heterozygous mutant fish in 0.016% tricaine (A5040, Sigma Aldrich) in embryo medium (EM ...
-
bioRxiv - Molecular Biology 2021Quote: WT MtbRho and the M495L mutant were overexpressed in Rosetta 2(DE3) cells (Merck-Millipore) harboring the appropriate pET28b derivative and purified following published protocols35 with minor modifications ...
-
bioRxiv - Molecular Biology 2020Quote: ... the mutant cells were treated with different dose of capmtothecin (CPT, Sigma, St. Louis, MO) and methyl methanesulfonate (MMS ...
-
bioRxiv - Microbiology 2019Quote: WT ppr-SiiA protein and mutant variants were produced using freshly transformed BL21(DE3) (Novagen) cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... pBMS50 for the K278R catalytically-inactive mutant) was transformed into Rosetta II (DE3) cells (Novagen), and overexpression and initial purification were performed as described in “General procedure for purification of H6-SUMO-tagged proteins,” with Ulp1 cleavage carried out at room temperature for 2 hr ...
-
bioRxiv - Microbiology 2020Quote: ... Wild-type and mutant VP40 bearing a Strep-Tag were cloned into pTriEx-5 (Novagen) and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus) ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
-
bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
-
bioRxiv - Molecular Biology 2020Quote: ... U224G mutants were grown in LB Broth with 50 μg/ml spectinomycin (Spec) (Sigma-Aldrich) and the RNase E mutants of the respective SgrS mutations were grown in LB Broth with 25 μg/ml kanamycin and 50 μg/ml spectinomycin ...
-
bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
-
bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
-
bioRxiv - Microbiology 2023Quote: ... FLAG-LANA mutants (N+C, N, C) were detected with Rabbit anti FLAG (Sigma 7425).
-
Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: Heterozygous mutant casper embryos were treated with 0.003% 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) from 24 hpf onwards to delay pigmentation ...
-
bioRxiv - Cancer Biology 2023Quote: ... after which TP53 mutant selection was achieved by addition of Nutlin-3A (Sigma-Aldrich, SML0580) to a final concentration of 5nM ...
-
bioRxiv - Cell Biology 2020Quote: ... at room temperature before dissociated cells were collected and cultured in RPMI medium containing 200nM TPA and 200pM cholera toxin (RPMI+ hereafter) (both Sigma-Aldrich). At passage 0 ...
-
bioRxiv - Physiology 2021Quote: Recipient mice were rendered diabetic before islet transplantation with a single intravenous injection of the beta cell toxin streptozotocin (STZ 200 mg/Kg; Sigma, MO) dissolved fresh in 100 mM sodium citrate (pH 4.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... University of Pennsylvania) and grown in serum-free WIT-P medium (Cellaria) without antibiotics and 100ng/ml cholera toxin (Sigma-Aldrich). Quality control of all cell lines was carried out by frequent STR analysis (Eurofins MWG) ...
-
bioRxiv - Cell Biology 2022Quote: ... Collected blebs were then pelleted with a centrifugation at 16100 g for 5 min and incubated in a solution containing an exogeneous ATP regeneration system (energy mix) and alpha-toxin to permeabilise the bleb membrane (5% A-Hemolysin alphatoxin (1 mg/mL; H9395, Sigma Aldrich), 2% energy mix (50 mg/mL UTP ...