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Citations for Millipore Sigma :
4901 - 4950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Duolink in situ proximity ligation assay was performed per manufacturer protocols (Sigma), and coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI.
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bioRxiv - Biochemistry 2024Quote: ... Cellular proliferation was analyzed using the XTT assay (Sigma Aldrich, MO, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Electrochemiluminescence (ECL) assay was performed using 2 ml HRP substrate (WBLUR0500, Millipore) for 3 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Protein content was determined by performing a BCA protein assay (Merck Millipore). SDS-PAGE was performed on a Mini-PROTEAN System (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... Unbuffered DMEM assay medium was composed of DMEM (Sigma #5030; pH 7.4) supplemented with 31.6 mM NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunoblots were visualized by treating membranes with Immobilon Western Chemiluminescent HRP substrate (Millipore, Burlington, MA), exposed ...
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bioRxiv - Cell Biology 2020Quote: Membranes were first blocked in 5% milk (Marvel) in PBS-1x Tween® 20 (Sigma) for 1 h at RT and incubated with primary antibodies overnight at 4 °C on a shaker platform (for a full list see Supplementary Table 3) ...
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bioRxiv - Cell Biology 2020Quote: ... Then the membrane was blocked in 5% w/v bovine serum albumin (BSA, Sigma-Aldrich) and incubated with blocking buffer diluted primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were blocked in non-fat milk with 0,1% Tween 20 for 1h (Sigma-Aldrich), and incubated with antibodies as indicated in Table S2 at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: WB were performed by standard procedures using polyvinylidene fluoride membranes (Immobilon-P polyvinylidene difluoride, Millipore), ECL Prime (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered twice through 0.45 μm PVDF membrane filters (Millipore, Billerica, MA, USA) to remove bacteria and concentrated with 30K Amicon filters (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were probed with the following antibodies: Tyrosine-Tubulin (Sigma-Aldrich T9028, 1:10,000), GAPDH (Santa Cruz Biotechnology sc-25778 ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were washed and incubated with anti-laminin antibody (L9393; Sigma-Aldrich 1:1000 dilution) followed by IRDye 800 CW dye-conjugated donkey anti-rabbit IgG (LI-COR ...
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bioRxiv - Developmental Biology 2020Quote: ... The membranes were incubated separately with anti-Mmp-9 (1:1000, Cat# AB19016, Merck Millipore), anti-Vegf (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... and the transfer quality was evaluated by staining the membrane with Ponceau S (Sigma-Aldrich). The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... from samples filtered on with a 0.2 μm Isopore membrane filter (Millipore Sigma, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with the antibodies as follows: antibodies for HSV tag (Millipore, 69171), SREBP-1 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with primary antibody (mouse anti-FLAG antibody (Sigma, F1804; 1:1000); mouse anti-Tubulin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to a 0.45 µm PVDF membrane (EMD Millipore, Billerica, MA, USA), and the blot was probed with anti-AGO2 mouse monoclonal antibody (Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins by SDS-PAGE were transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore), washed with TBST for 5 minutes and then were blocked with 5% BSA in TBST[0.1%(v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate [EMD Millipore AP160P] ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were immunoblotted with the following antibodies: mouse anti-β-actin (AC-15, Sigma #A5441), mouse anti-β-catenin (cl14 ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto a polyvinylidene fluoride membrane (Immobilon R, Merck Millipore Ltd) for 1 hour at 20 V ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) and concentrated with an ultrafiltration unit using a 10-kDa cutoff membrane (Millipore). The concentrated protein was flash-frozen in liquid nitrogen and stored at -80°C.
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bioRxiv - Cell Biology 2022Quote: ... for SDS-PAGE and transferred using the semidry transfer method to PVDF membranes (Millipore, IPFL00010). The PVDF membranes were incubated in TBS containing 5% nonfat milk and 0.05% Triton X-100 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... Media components were mixed and sterile-filtered through a 0.22 um PES membrane filter (Millipore).
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane and probed with anti-HA-HRP (3F10) (Sigma), mouse anti-GFP (ab6556 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins samples were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore) for 16 h at 100 mA ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were subsequently transferred onto a PVDF membrane and FLAG epitopes were detected by Sigma M2 antibodies followed by ECL imaging.
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bioRxiv - Developmental Biology 2021Quote: ... The protein was then transferred from the gel to a PVDF membrane (Immobilon-P, Millipore) in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed several times and analyzed with chemiluminescence using ECL Lumina Forte (Millipore, WBLUF0500) using the Chemidoc XRS Imager (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... A 5 μm pore diameter polycarbonate isopore membrane filter (22 μm thickness) (Millipore, Burlington, MA) was stacked on top of a 0.2 μm membrane (Pall Corp ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated retina (dorsal part) was then mounted on a 0.22 mm membrane filter (Millipore) with a pre-cut window to allow light to reach the retina and put under a two-photon microscope (Bruker ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane that was blocked with 5% skim milk (Sigma) in PBS overnight and incubated with rabbit antiserum against ACS (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... and placed on 30-mm hydrophilic PTFE membranes with 0.4 µm pores (Merck, Millipore, Ireland). Membranes were inserted into 35-mm Petri dishes containing 1 ml of culture medium and cultures were maintained up to 25 days in an incubator at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Then bacteria were filtered from the culture media with 0.22 μm membrane filter (Millipore, MA) using vacuum filtration (Berg and Turner ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with secondary antibody (Horseradish peroxidase conjugated) (1:2000 dilution, #AP132P Sigma) for 1 hr at RT ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
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bioRxiv - Bioengineering 2021Quote: ... Each sample was sealed in a benzoylated dialysis membrane (MWCO ∼ 2 kDa; Sigma, Cat.# D2272) and dialyzed against sodium acetate buffer at 37°C for 48 h ...
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bioRxiv - Bioengineering 2021Quote: ... membranes were fixed in the lung on a chip device with 10% formalin (Sigma Aldrich) and 2.5% glutaraldehyde (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with the anti-rabbit-IgG conjugated with horseradish peroxidase (Sigma Aldrich) at room temperature at 1:10,000 dilution for 2 hr ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were separated by 10% SDS-PAGE and transferred to a Nitrocellulose membrane (Millipore, #HATF00010). The membranes were incubated with anti-UCP1 (rabbit polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... resolved protein bands were transferred onto Polyvinylidene Fluoride membranes (PVDF, 0.45μm, EMD Millipore, ON, Canada) for 75 minutes on ice in a transfer buffer containing 25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were kept on sterile 0.45 μm Omnipore membrane inserts (Millipore, cat no. FHLC01300) in an incubator (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed again in TBST and incubated in Immobilon Western Chemiluminescent HRP (Sigma-Aldrich, Burlington ...
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bioRxiv - Cancer Biology 2020Quote: ... and were then transferred onto polyvinylidene difluoride membranes (IPVH00010, pore size: 0.45 mm; Millipore Sigma). Membranes were blocked for 1 hour in 5% bovine albumin serum (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were blocked with 5% non-fat milk in 0.1% PBS-Tween 20 (Sigma) for 1 hr at room temperature and then incubated with the following primary antibodies (diluted in 5% non-fat milk in 0.1% PBS-Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... PVDF membranes were incubated in 1X Casein blocking buffer (10X stock from Sigma-Aldrich, B6429) made up in TBS ...