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Citations for Millipore Sigma :
4801 - 4850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... prior to SDS-PAGE and transfer to Immobilon-FL membranes (Millipore Sigma, Burlington, MA). Blots were blocked with Intercept TBS blocking buffer (LI-COR Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... and the proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, #IPVH00010). Immunoblots were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2024Quote: ... followed by transfer to 0.2 µM pore size PVDF membrane (Millipore; Billerica, MA, USA). Membranes were blocked with 1 hour incubation in 5% milk in PBS ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: ... Following gel electrophoresis proteins were transferred to an Immobilon-P PVDF membrane (Merck Millipore) using the Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was processed for chemiluminescence detection using Luminata Forte Western HRP Substrate (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 4°C) and filtering the soluble supernatant through a 0.22 μm polyethersulfone membrane (Millipore) to ensure sterility (Segeritz and Vallier ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by electro-blotting onto a PVDF membrane (PVDF-PSQ or PVDF-FL, Millipore). The following antibodies were used for detection at a dilution of 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysate was separated by Bis-Tris-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked for 1 h and incubated using the indicated antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... membranes were blocked for 1 h in Blocking Buffer (0.1 % casein (Sigma-Aldrich, E0789) in 0.2 x PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a wet transfer onto Immobilon-P Polyvinylidene Fluoride (PVDF) membrane (Merck Millipore) using the Mini Trans-Blot Cell system (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were subsequently transferred from gels to Immobilon-P PVDF membranes (IPVH00010, Millipore) and blocked in 5% milk in TBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase (Sigma Aldrich) at a 1:10,000 dilution in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed and incubated with anti-laminin (LAM-89) (1:100; Sigma-Aldrich) primary antibody and the corresponding secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 60 minutes at RT using casein-blocking buffer (Sigma-Aldrich) and then probed overnight with primary antibodies diluted in TBST (110mM Tris-HCl ...
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bioRxiv - Genetics 2024Quote: ... separated in SDS-PAGE gels and transferred onto PVDF membranes (0.45 µm, Merck Millipore). Membranes were incubated in 5% BSA for 1 h and subsequently incubated with primary and fluorescently-labeled secondary antibodies (Supplementary Tables S2 and S3 ...
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bioRxiv - Biophysics 2021Quote: ... and streptavidin (used for the LNP detachment assay) were from Sigma-Aldrich. Citrate buffer (Teknova ...
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bioRxiv - Cell Biology 2020Quote: ... PBS was then removed and OPD assay solution (Cat # P9187, Sigma-Aldrich) was added for 30 minutes in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... cell proliferation and cell death assays were anesthetized using 1x benzocaine (Sigma) and fixed overnight in 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2021Quote: ... were subjected to a PI3K activity assay (catalog no. 17-493; Millipore), as detailed in the manufacturer’s protocol manual.
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bioRxiv - Molecular Biology 2020Quote: PLA assay was performed following Duolink® PLA Fluorescence Protocol (Sigma-Aldrich). Cells were grown ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2020Quote: ... kinase assays were prepared using C18 ZipTips (EMD Millipore, Etobicoke, ON, Canada). Samples in 50% acetonitrile and 0.1% formic acid were introduced into a linear ion-trap mass spectrometer (LTQ XL ...
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bioRxiv - Bioengineering 2020Quote: ... DNA content was quantified with Hoechst Bisbenzimide 33258 dye assay (Sigma-Aldrich) as described previously [31] ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was recovered and proteins were quantified with Bradford assay (Sigma Aldrich) and samples were stored at -80°C until further used ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell survival was measured by using the colorimetric MTT assay (Sigma-Aldrich) as we previously performed (11).
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bioRxiv - Neuroscience 2020Quote: ... after which chromatin immunoprecipitation assays were performed according the manufacturer’s instructions (Millipore). Detailed information about primers used for quantitative PCR is provided in Supplementary Table 1.
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bioRxiv - Biochemistry 2021Quote: ... protein expression and purification and enzyme assays were obtained from Sigma-Aldrich, TCI chemicals ...
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bioRxiv - Biochemistry 2020Quote: eNAD-based NADase assay: eNAD (Nicotinamide 1,N6-ethenoadenine dinucleotide, SIGMA – N2630) was solubilized in water and mixed with native NAD+ in ration of 1:10 (mol:mol ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting glucose was assayed using glucose HK assay reagent (Sigma G3293) in microtitre plates using a SPECTROstarNano (BMG LABTECH ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Molecular Biology 2021Quote: Cell migration assay was performed using 24 well chambers (Sigma CLS3422-48EA) with uncoated polycarbonate membranes (pore size 8µm) ...
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bioRxiv - Cancer Biology 2020Quote: Succinate levels were determined using a colorimetric assay (Sigma-Aldrich Cat # MAK184) according to the manufacturer’s specifications.
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bioRxiv - Cell Biology 2021Quote: ... kinase assays were prepared using C18 ZipTips (EMD Millipore, Etobicoke, ON, Canada). Samples in 50% acetonitrile and 0.1% formic acid were introduced into a linear ion-trap mass spectrometer (LTQ XL ...
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bioRxiv - Molecular Biology 2022Quote: ... and it was determined by BCA protein assay (Sigma, Sao Paulo, Brazil). A total of 10 µg protein concentrate was used in a 10% or 12% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Bioengineering 2022Quote: ... Ammonium concentration was determined via an enzymatic spectrophotometric assay (Sigma cat#AA0100). Average daily growth rate (μmax) ...
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bioRxiv - Neuroscience 2022Quote: ... Total serum calcium levels were obtained using a colorimetric assay (Sigma, MAK022). The results were read by a Nano Quant plate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: For the trehalose assays we incubated samples overnight with trehalase (#T0167, Sigma). Absorbance was measured in a plate reader (BMG Labtech ...
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bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
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bioRxiv - Neuroscience 2022Quote: ... protein content was additionally measured using the Bradford assay (Sigma-Aldrich, USA). Bovine serum albumin dissolved in 1xPBS was used for the standard curve ...
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bioRxiv - Systems Biology 2021Quote: ... Metabolite standards and internal standards for assays include trans-zeatin (Sigma-Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich) with the previously described method (Xiong et al. ...
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bioRxiv - Systems Biology 2021Quote: ... and phospholipids (PL) levels by colorimetric assay from Sigma (St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ADP content was measured with a fluorimetric assay (Sigma Aldrich, ref. MAK033).
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bioRxiv - Synthetic Biology 2020Quote: ... for 2hrs and detected using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Microbiology 2021Quote: ... The resulting lysates were then used in the aconitase assay (Sigma-Aldrich) in a 96 well plate format according to the kit instructions ...
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bioRxiv - Biochemistry 2020Quote: ... reagents used in the coupled enzyme assay (Sigma-Aldrich, Oakville, ON Canada).
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bioRxiv - Cancer Biology 2020Quote: ... when viable cell numbers were determined using the Guava ViaCount assay (Millipore) or CellTitreGlo (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... For differentiation assays promastigotes were grown in Grace’s insect medium (Sigma-Aldrich) with 10% HIFCS (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Other mammalian IgGs used in this assay were purchased from Sigma-Aldrich or donated as described above.
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bioRxiv - Immunology 2020Quote: ... The purified protein content was measured by Bradford assay (B6916, Sigma-Aldrich) and analyzed under reducing conditions with 12% SDS-PAGE ...