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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... All membranes were incubated with α-tubulin serving as loading control (Sigma #T5168, 1:5000) for one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... donor cells were recommended for optimal staining with the fluorescent membrane dye PKH26 (Sigma-Aldrich) for 5 minutes according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then probed overnight (4°C) with 1:1000 anti-Puromycin antibody (MABE343, Millipore) in 5% milk TBST with gentle rotation ...
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bioRxiv - Cell Biology 2020Quote: ... A membrane filter (Sigma-Aldrich Company Ltd., Dorset, UK; MF-Millipore, 0.22 µm pore size) was placed between the syringe and the dispensing tip (Intertronics ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s instructions (Avanti Polar Lipids, USA; membranes were purchased from Sigma, Switzerland). Osmolarities of CDV hosting solutions were determined with an osmometer (Osmomat 3000basic ...
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bioRxiv - Microbiology 2020Quote: ... Outer membrane proteins of these bacteria were subsequently labelled with fluorescein isothiocyanate (FITC, Sigma-Aldrich) as previously described [57] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel followed by electrotransfer to PVDF membranes (Millipore). The membranes were blocked with TBST supplemented with 10% skim milk for 30 mins at room temperature and further probed with primary antibodies at 4°C overnight including Trim17 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... The membranes were blocked in either 5% w/v milk or 2.5% BSA (Sigma, A3608) in PBS ...
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bioRxiv - Biophysics 2021Quote: ... for 1 hour then filtered using 0.22 μm Millex-GP PES membrane filters (Millipore SLGP033RS). The clarified lysate was loaded onto a HisTrap FF Crude column (Cytiva 29048631 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by SDS-polyacrylamide gel electrophoresis and transferred onto PVDF membranes (IPVH00010, Millipore). Membranes were blocked in 5% milk before adding antibodies of interest ...
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bioRxiv - Cancer Biology 2021Quote: ... 12-16% or 16% SDS-PAGE gels and transferred to Immobilon-P PVDF membranes (Millipore) with 0.45 um (CA9 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blotted with rabbit polyclonal anti-SNAP-25 (1:1000, Millipore Sigma #S9684) in TBST + 5% NFM and HRP-linked goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... membranes were incubated overnight at 4 °C with anti-SDHB (1:1000; Sigma-Aldrich, #HPA002868), anti-DNMT1 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-PSQ membrane (0.2 µm; Millipore). Immunoblotting was performed using the following primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed in milliQ water and protein transfer was checked by Ponceau staining (Sigma). Transferred membranes were washed once with TBS and three times with TBS-Tween (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were transferred onto a buffered poly-vinilidine di-fluoride (PVDF) membrane (Millipore, Germany). The non-specific staining was blocked by 5% skimmed milk protein (1X TBST;4hr ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were exposed to the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA). Blots were detected on X-ray film using the ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were incubated with antibodies specific for ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed in Tris-buffered saline with 0.1% (vol/vol) Tween 20 (TBST, Sigma) before blocking in 5% (wt/vol ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to PVDF membranes at 70V for 2 hours or overnight (Immobilon P, Millipore). Ponceau S staining was used to verify even transfer (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked with 1% w/v bovine serum albumin (BSA, Cat. No. A7906, Sigma) and 5% w/v skimmed milk ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE and transferred to a PDF membrane (Millipore, Billerica, MA). The membrane was blocked with PBS containing 0.5% tween (PBST ...
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bioRxiv - Plant Biology 2022Quote: Protein samples were run on SDS-PAGE and transfer onto PVDF membrane (Immobilon-P, Millipore). The membrane was blocked by PVDF blocking reagent (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were then rinsed in deionized water and prehybridized with PerfectHybTM Plus Hybridization Buffer (Sigma) at 42°C for 20-60 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 7,4’-dihydroxyflavone and the plasma membrane microsomes using nitrocellulose filters (0.45 mm pore-size; Millipore). The transport assays were performed with microsomes corresponding to 520 ng·μl-1 protein concentration mixed with transport buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then probed with primary antibodies specific for murine caspase-11 (#C1354; Sigma-Aldrich), caspase-3 (#9662 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed three times with TBS-T (Tris-buffered-saline/0.1% Tween 20 (Sigma)) and incubated overnight at 4°C with primary antibodies in 2% BSA/TBS-T (0.1%) ...
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bioRxiv - Neuroscience 2022Quote: ... 10% or 15% SDS polyacrylamide gels and transferred to a PVDF (polyvinylidene fluoride) membrane (Millipore) at 100 V for 2 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then transferred to Immobilon-P-polyvinylidene difluoride (PVDF) 0.45 μm membranes (Millipore, USA) for 55 minutes at 80 mA per blot using a semi-dry transfer cell ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed in Tris-buffered saline with 0.1% (vol/vol) Tween 20 (TBST, Sigma) before blocking in 5% (wt/vol ...
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Morphine-induced side effects can be differentially modulated by cannabidiol in male and female ratsbioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by a filtration with a 0.45 µm Durapore membrane filter (Millipore, São Paulo, Brazil). Finally ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered twice through 0.45 μm PVDF membrane filters (Millipore, Billerica, MA, USA) to remove bacteria (confirmed by colony counts ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Neuroscience 2024Quote: ... before being transferred onto Immobilon-P polyvinylidene fluoride membranes (#IPVH00010, 0.45 mm pore size; Millipore) in NuPAGE™ Transfer Buffer with methanol 20% (NP0006 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The isolated retina was mounted on a small piece of filter paper (MF-membrane, Millipore), in which a 2 × 2 mm window had been cut ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was washed again before developing with Luminata Classico Western HRP substrate (Millipore, #WBLUR0100) Luminata Classico Western HRP substrate (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck, Sigma Aldrich) at 50°C in rotation ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were immunoblotted with primary antibodies against actin (1:30,000, Sigma Aldrich, Stockholm, Sweden) and TH (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... we had to switch to a 1 µm PTFE membrane (Omnipore, Merck Millipore, Darmstadt, Germany). Filters were stored on dry ice or −80 °C until DNA extraction.
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked for 1 hour in blocking buffer [3% Skim milk (Sigma-Aldrich) in T-TBS buffer (0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... The crude was purified by dialyzing (membrane cutoff = 12–14 kDa) against DMF (Sigma-Aldrich) for 24 h by changing the DMF every 8 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the membranes were incubated with chemiluminescent HRP substrate (Cat. No. WBKLS0500, Millipore Sigma, Billerica, MA), and were recorded using the ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blotted with anti-GFP monoclonal primary antibody (mouse monoclonal, Sigma- Roche #11814460001) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with either 4% non-fat milk or fish gelatin (Sigma #G7041-100G) for 30 min at room temperature and incubated overnight in primary antibodies on an orbital rocker at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... membranes were washed with 1X TBS-T and revealed with HRP substrate (Immobilon Forte, Millipore). Images were acquired using the ChemiDoc MP Imaging System (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with mouse anti-FLAG (1:5,000) (cat #3724, Sigma, St Louis, MO), mouse anti-tubulin (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... run for 80 minutes at 50V then blotted onto PVDF membrane (Millipore, Bedford, MA, USA) overnight at 4°C.
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bioRxiv - Neuroscience 2022Quote: ... All media were filtered through a polyethersulfone membrane with a 0.22 μm pore size (Millipore). All cortical neuron cultures were seeded in co-culture with a confluent layer of primary rat cortical astrocytes (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... and membranes were immediately washed in distilled water and stained with ponceau (P7170-1L, Sigma) Membranes were then blocked by incubation with 5% (w/v ...