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Citations for Millipore Sigma :
4901 - 4950 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2021Quote: ... dried under a stream of nitrogen and reconstituted in 25 μL of chloroform:methanol (2:1, vol/vol) and loaded on a TLC plate (Sigma Aldrich Cat #1118450001). The plate was activated using 1.8% (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... and 10 μCi [γ32P]-ATP in reaction buffer (25 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 0.5 mM CaCl2, 2 μM CaM [Sigma-Aldrich], 1 mM DTT) at 30°C for 30 min to perform the vitro kinase assay ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Systems Biology 2021Quote: Embryos which were to be used in immunofluorescence experiments were grown in egg water supplemented with 0.003% 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, Cat# P7629) from 24 hpf to prevent pigmentation ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of freshly made 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 1 mg/mL; Sigma-Aldrich, St. Louis, MO) in serum-free ...
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bioRxiv - Neuroscience 2020Quote: ... These sections were placed in a blocking buffer (2% normal donkey serum and 1% bovine serum albumin in PBST; Sigma A7906-100G) for 30min ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM 2-mercaptoethanol) and concentrated up to 1 nmol/ml (1A260 = 69 pmol) using Amicon Ultra centrifugal filters (Millipore, MWCO 100,000). Further ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays; F1804; Sigma, St. Louis, MO, USA). Primers and sequence information are provided in Supplementary Table S1.
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bioRxiv - Physiology 2020Quote: ... Frozen tissue was sectioned into 2 mm coronal sections and a 1 mm diameter biopsy tool (Harris Micro-punch, Millipore-Sigma, Z708658) was used to collect tissue punches in ipsilateral regions distal and proximal to the infarcted tissue similar to IHC regions (Figure 3B) ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 2% (v/v) Vitox® (Oxoid Ltd) and 15 μg mL−1 of porcine haematin (ferriprotoporphyrin IX hydroxide, Sigma-Aldrich). Exposure to non-growth conditions was minimized by maintaining suspensions and diluents at 37°C in heat block with sand or benchtop incubator.
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bioRxiv - Microbiology 2020Quote: ... the purified proteins were concentrated to 1-2 ml in phosphate buffered saline (PBS, pH 7.4) by 30K Amicon Ultra-15 (Millipore Co Ltd., USA). Concentrations of these proteins were determined by Bradford Protein Assay Kit (Beytotime Bio Co Ltd. ...
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bioRxiv - Cell Biology 2020Quote: ... IMR90 cells (n=2 biological replicates) stably expressing ZNF416-FLAG were cross-linked with 1% formaldehyde (Sigma Aldrich, St. Louis, MO, USA) for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells used for total protein purification were lysed with 2 ml of a buffer containing 1% Sodium dodecyl sulfate (SDS; Sigma-Aldrich #436143), 50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times with PBS and incubated 2 h at RT in the dark with FITC-coupled α-rabbit antibodies (1:200 in BSA-PBS, Sigma Aldrich). After washing and drying ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.1 (final urea concentration < 2 M) before addition of trypsin (1 µg per 50 µg of protein; Sigma-Aldrich Cat# T6567) for a 3 h incubation ...
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bioRxiv - Plant Biology 2021Quote: ... Immunodetection was performed by incubating the immunoblot for 2 h with anti-mouse IgG conjugated with horseradish peroxidase (1:10,000 dilution; Millipore; Catalog # 12-349). Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Systems Biology 2022Quote: ... the media was replaced with MEM-ɑ media supplemented 10% FBS plus (1) rosiglitazone or (2) 0.517 mM IBMX (Sigma Aldrich, USA), 62 nM dexamethasone (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... or LTB in 50 mM carbonate coating buffer (NaCO3, NaHCO3, 0,1% NaN3) or 2 μg/mL GM-1 ganglioside (Sigma, St. Louis, Mo) at 4 °C overnight or at room temperature for 2 hr ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich, P8833). The RNAi consortium clone IDs for the hairpins used were ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Eggs were left to desiccate for 1-2 min before being covered in halocarbon oil 700 (Sigma Aldrich, Castle Hill, NSW Australia). Eggs were injected using a MINJ-1000 microinjection system (Tritech Research ...
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bioRxiv - Plant Biology 2020Quote: ... The same incubation and A340nm measurements were performed after adding 2 μL of yeast invertase solution (100 mg ml−1, Sigma-Aldrich®) diluted in buffer (solution 1 ...
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bioRxiv - Immunology 2020Quote: ... Isolated CP colonies (1-2 colonies) were sub-cultured in 50 mL tubes containing thioglycolate broth with resazurine (Sigma-Aldrich®, MO) supplemented with 2% beef extract (Bioworld ...
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bioRxiv - Cell Biology 2020Quote: ... extracted MT arrays were placed in imaging buffer (1 mM ATP, 2 uM Paclitaxel, 0.133 mg/mL casein (Sigma Aldrich, C5890-500G), 0.133 mg/mL bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were washed 3 times with PBS and incubated at room temperature for 2 hrs with Cy3-conjugated goat anti-rabbit IgG antibody (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted (600g, 2 minutes) and resuspended in 1 ml SC-Full and supplemented with 10 μl Fluorescent Brightener 28 (Sigma-Aldrich F3543). Cells were incubated for 5 minutes in the dark and pelleted (600g ...
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bioRxiv - Cell Biology 2021Quote: ... 1 volume of AluI-stock was added to 2 volumes of 5 mg ml−1 fluorescein (FITC) labelled 500 kDa dextran fraction (Sigma-Aldrich, FD500S) dissolved in injection buffer (50 mM K-HEPES ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were co-incubated with β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH; 1 mM; N7505, Sigma-Aldrich) and HET0016 (10 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... in Spectra/PorTM 1 RC Dialysis Membrane Tubing (SpectrumTM 132650) and concentrated in Amicon ®Ultra-2 mL centrifugal filters (Millipore UFC205024).
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bioRxiv - Genetics 2022Quote: ... similar ten-fold serial dilutions were performed and equal volume from each dilution was plated on solid minimal medium (CO2) containing 1) either no MESNA (2-MercaptoEthane Sulfonate Na, Sigma, M1511-25G), or 2 ...
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 500 to 1000 μl L-15 full medium were added to the suspension and the solution was seeded on 1 - 2 peanut lectin (Sigma-Aldrich, USA) coated glass bottom petri dishes (ibidi ...
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bioRxiv - Microbiology 2024Quote: ... Each strain was grown in liquid mineral medium (LMM) [43] supplemented with 2 g.L -1 of 99% 13C6-glucose (Sigma-Aldrich, Munich, Germany) as a sole carbon source ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... cells were permeabilized in 0.5% v/v Triton X-100 for 5 min in 1x DPBS and blocked with 1% BSA in IF wash buffer (1% w/v BSA, 2% v/v Triton X-100; 0.41% v/v Tween-20, EMD Millipore, 1x DPBS) for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Bioengineering 2024Quote: ... cells were permeabilized in 0.5% v/v Triton X-100 for 5 min in 1x DPBS and blocked with 1% BSA in IF wash buffer (1% w/v BSA, 2% v/v Triton X-100; 0.41% v/v Tween-20, EMD Millipore, 1x DPBS) for 1 hr.
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bioRxiv - Cell Biology 2024Quote: ... lentiviral particle production and viral transduction LV-ShOpa1 clone #1 (TRCN0000091111) and LV-SHOpa1 clone #2 (TRCN0000348537) (Sigma, St. Louis, MO, USA) and pLKO.1 ShCtrl and psPAX2 (#12260 ...
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bioRxiv - Cell Biology 2024Quote: ... Staining Solution was prepared by freshly diluting 2 μL of LTR stock solution (1 mM LysoTracker Red DND-99; Sigma Aldrich, L7528) in 1 mL of medium ...
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bioRxiv - Neuroscience 2023Quote: ... OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα) (1:4000) (Supplementary Table 2) and then seeded on coverslips coated with poly-D-lysine (20 μg/ml) (Sigma-Aldrich; P0899) and incubated at 37°C and 10% CO2 in Sato medium ...
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bioRxiv - Molecular Biology 2023Quote: Total fatty acids were extracted from the liver using a 1:2 (v/v) mixture of chloroform/methanol in the presence of 10 nmol of tridecanoic acid (Sigma-Aldrich #T0502) and 10 nmol of phosphatidylcholine (PC ...
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bioRxiv - Neuroscience 2023Quote: ... 1:125 in blocking buffer for 2 hours and tertiary antibody solution (streptavidin-horseradish peroxidase conjugated, 1:250 in blocking buffer, Sigma-Aldrich, RPN1231V) for 2 hours before being exposed to DAB (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Republic of Korea) and cultured in micro-drops of KSOM medium with 1/2 amino acids (EmbryoMax®; EMD Millipore, Billerica, MA) in a humidified atmosphere of 5% CO2 at 37°C until use.