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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Bioengineering 2022Quote: ... A 2 μL aliquot of 100 μM aptamer in PBS was thawed and mixed with 4 μL of 10 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) for 8 hours to reduce the disulfide bond ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...
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bioRxiv - Immunology 2020Quote: ... Then the slips were washed with PBS for three times, and after that the hemocytes were stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/ml) (Sigma, USA) for 5 min [55] ...
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bioRxiv - Neuroscience 2020Quote: ... Embryos at 2 dpf (days post-fertilization) were removed from their chorions and anesthetized with 4% tricaine (MS-222, Sigma Aldrich). The embryos were placed in a 1% agarose-coated dish with small holes ...
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bioRxiv - Microbiology 2021Quote: ... cells from control and experimental culture were washed with PBS and mounted with DAPI (4’,6-diamidino-2-phenylindole) over glass slides using slide mounting media from Sigma-Aldrich and observed under the fluorescent microscope ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining 2 ml were added to falcon tubes containing 4 ml of 6 M GndHCl (Sigma Aldrich, Saint Louis, Missouri) and mixed thoroughly to denature the samples ...
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bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed again for three times before the final staining of the nuclei using 4’,6-diamidin-2-phenylindol (DAPI, Sigma-Aldrich). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then dried in 80% ethanol and mount on a glass slide with antifadent AF1 (Citifluor, USA) added with 4’-6’-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) for counterstain at a final concentration of 2 μg.ml−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Plant Biology 2021Quote: ... rinsed with 3 mL of medium and washed off into new Eppendorf tubes with 600 μL of boiling buffer (100 mM Tris-HCl, 4 mM EDTA, pH 7.5, autoclaved and supplemented with phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726)) ...
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bioRxiv - Developmental Biology 2020Quote: ... After 4 days, cells were switched to DMEM/F-12, HEPES (31330038, Thermofischer Scientific) with 2% Horse serum (H1270, Sigma-Aldrich). Before staining ...
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bioRxiv - Cell Biology 2020Quote: ... C9H2Cl4O2 was purchased from Tebu-Bio and used at 2μM working concentration. Monastrol (Ref. M8515) and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Ref. D8417) were purchased from Sigma-Aldrich. S-Trityl-L-cysteine (STLC) ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then counterstained (5 min) with 0.5 µg/ml 4’,6’-diamino-2-phenylindole (DAPI; Sigma-Aldrich® Cat#D9542) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Bioengineering 2021Quote: BMOs were collected from the array as described above and fixed with 2 % or 4 % PFA or 10 % Formalin (Sigma, HT501128) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... central back skin of K14ΔNβ-cat x PDGFRαH2BEGFP transgenics was clipped and treated topically with 100 µl 4-Hydroxytamoxifen (4OHT) (2 mg/ml dissolved in acetone; Sigma-Aldrich) every second or third day for a total of 6 applications before and after sham or acUVB exposure (see experimental design in Figure 5C) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... was changed daily and animals over 4 days post fertilisation were euthanized by overdose of MS-222 (300 mg/l) or 15% 2-Phenoyethanol (77699 SIGMA-ALDRICH) at the end of experiments.
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bioRxiv - Cell Biology 2023Quote: ... Minced samples were transferred into a digestion buffer (4 mL digestion buffer per 100 mg of muscle) containing 2 mg/ml Dispase II (D4693 Sigma-Aldrich) and 2 mg/ml Collagenase IV (17104019 Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Nuclear DNA integrity and gross cell morphology in dPPM was assessed with 4’,6’- diamidino - 2 - phenyl indole (DAPI, Sigma-Aldrich) staining and fluorescence microscopy (Nikon Eclipse Ti ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, 10 μg/ml, Cat.No.: D27802, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μm tissue sections (2-4 sections per donor) were fixed with 10% neutral buffered formalin solution (catalog no. HT501128, Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... or MC38 murine colon adenocarcinoma cells (2 × 105) into their shaved flanks (Day 0) and intraperitoneally injected with tamoxifen (4 mg/mouse in corn oil) (Sigma-Aldrich) on day 0 ...
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bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Systems Biology 2022Quote: ... cells were washed with PBS and their nuclei were stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride) and lysed by the addition of 10 mg hen egg white lysozyme (Sigma, L6876) and sodium deoxycholate (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... they were cryopreserved for 48h at 4°C in a 30% sucrose solution and subsequently frozen with 2-methylbutane solution (Sigma-Aldrich). Parallel free-floating coronal sections (30-µm-thick ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then washed again for 2 min with the same 4% poly-acrylamide (PA) gel solution supplemented with the polymerizing agents ammonium persulfate (Sigma, A3678) and TEMED (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in DMEM without FBS and red phenol supplemented with 2 mM L-glutamine and 25 µM verapamil (V4629, CAS 152-11-4, Sigma Aldrich) for 1 h at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Neuroscience 2023Quote: ... buspirone hydrochloride (1078-80-2) and all-trans retinal (116-31-4) were purchased from Sigma Aldrich (St Louis, MO, USA).(Dunham & Venton ...
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bioRxiv - Bioengineering 2023Quote: ... Each EC-patch was transferred by a wide bore pipette tip into 50 μL EGM-2 medium containing 4 U/mL thrombin (Sigma, T9549). Then the medium with a patch was mixed with 50 μL fibrinogen stock solution ...
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bioRxiv - Genomics 2022Quote: ... bead/chromatin complexes were washed twice with Low Salt Wash Buffer at 4°C (Final 20 mM Tris-HCl, pH 8,0; 2 mM EDTA; 150 mM NaCl (Sigma-Aldrich, S6546); 1% Triton-X ...
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bioRxiv - Microbiology 2022Quote: ... counter-stained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 minutes at room temperature and mounted with Fluoroshield (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed in PBS and stained with 5 µg/ml 4′,6-Diamidino-2- phenylindole (DAPI) (D9564-10MG, Sigma-Aldrich) in 1xPBS+0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... 12-well non-tissue culture treated plates were coated with 32 μg/mL retronectin (WEHI) in PBS solution overnight at 4°C then blocked with 2% bovine serum albumin solution (#A1595 Sigma-Aldrich) in PBS at 37°C for 30 min were prepared ...