Labshake search
Citations for Millipore Sigma :
4801 - 4850 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
-
bioRxiv - Developmental Biology 2023Quote: We scanned formalin-fixed whole kidneys stained for 2 hours in 4% OsO4 solution (Sigma-Aldrich, St. Louis, MO, USA) using a previously developed method 31 ...
-
PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
-
bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
-
bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
-
bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
-
Physical activity increases neuronal activity in the circadian clock of diurnal Arvicanthis ansorgeibioRxiv - Neuroscience 2024Quote: ... The brain was then removed and fixed for 2 days in 4% paraformaldehyde containing C6FeK4N6·3H20 (Sigma-Aldrich, Lyon, France). Coronal slices (40 µm thick ...
-
bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
-
bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich, cat.no. 59427C-100ML). HEK293T-LentiX (Takara Bio ...
-
bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
-
bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
-
bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
-
bioRxiv - Neuroscience 2022Quote: ... 500 μg of total protein from PC12 cell extracts were incubated over night at 4°C with 2 μg of the phospho- tyrosine 4G10 (Sigma-Alrich/Upstate Cat# 05-321 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
-
bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
-
bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS and the cells’ nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL, Sigma-Aldrich) for 5 min at room temperature in the dark ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min ...
-
bioRxiv - Cell Biology 2024Quote: ... the respective secondary antibodies were incubated in ADB at room temperature in the dark for 90 min together with 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich). Before imaging the cells were washed again using PBS ...
-
bioRxiv - Molecular Biology 2024Quote: MM-NLP particles isolated from both sucrose gradient sedimentation (native) and GraFix (crosslinked) were concentrated to 2 µM using Amicon Ultra-4 centrifugal filters (Ultracel 30K, Millipore). C-Flat 1.2/1.3 (Cu ...
-
bioRxiv - Neuroscience 2024Quote: ... coverslips were incubated for 1 h in blocking medium containing the appropriate secondary antibody and 0.1 μg 4’,6-diamidine-2’-phenylindole (DAPI; Sigma-Aldrich). After three washes with PBS-T plus one additional washing step with PBS ...
-
bioRxiv - Neuroscience 2024Quote: All experiments were performed in the adapted BRUCE-4 mouse embryonic stem cell (ESC) line (Millipore Sigma #SF-CMTI-2). This line is derived from a male of the C57/BL6J mouse strain ...
-
bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
-
bioRxiv - Microbiology 2024Quote: ... Cell free supernatants were applied to anti-GFP mAb-Magnetic Beads (D153-11, MLB) for 2 hours at 4°C or anti-FLAG M2 Affinity Gel (A2220, Sigma) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified by centrifugation and incubated for 2 hours at 4 °C with anti-FLAG M2 agarose beads (Sigma) with gentle rotation ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... flies were homogenized in 50 μl PBS per fly (between 2-4 flies per sample) containing 0.1% Tween-20 (Sigma #1379) using a TissueLyser LT (Qiagen ...
-
bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
-
bioRxiv - Immunology 2024Quote: ... Next, 50 μl of 2× STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA (cat. E3889, Sigma-Aldrich) and 100 μg/ml RNase A (cat ...
-
bioRxiv - Neuroscience 2024Quote: ... we immersed the brain slices for 45 minutes at 4 °C in 2% Bovine Serum Albumin solution (BSA; Sigma-Aldrich) and 0.3% triton X-100 (ThermoFisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells from 2 brains (n = 4 cortices) were plated in a T-75 flask coated with poly-d-lysine (Sigma) in 15 mL of DMEM/F12 (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... A total of 2.5 × 105 HEK-293 cells were seeded onto poly-L-lysine (PLL)-coated 35 mm glass-bottom confocal dishes (SPL) with 2 μg/mL tau–mCherry plasmids and 4 μg/mL polyethyleneimine (PEI) in DMEM (Sigma) supplemented with 10% FBS (Welgene) ...
-
bioRxiv - Cancer Biology 2024Quote: ... #4 inguinal mammary glands were fixed in 4 % PFA overnight and then stained for 48 hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate ...
-
bioRxiv - Cell Biology 2024Quote: ... Fluorescent phalloidin was used to stain filamentous actin and DAPI (4′,6-diamidino-2-phenylindole) was used to stain nuclei (Sigma). Of note ...
-
bioRxiv - Developmental Biology 2024Quote: ... satellite cells isolated from ArpC2cKO mice were cultured in GM supplemented with or without 2 μM 4-hydroxytamoxifen (Sigma; H6278) for 10 days ...
-
bioRxiv - Neuroscience 2021Quote: The mGluR5 specific negative allosteric modulator CTEP (chloro-4-((2,5-dimethyl-1-(4-(trifluoromethoxy)phenyl)-1H-imidazol-4-yl)ethynyl)pyridine) was purchased from Sigma-Aldrich (SML2306) and administered at 2 mg/kg concentration by intraperitoneal (i.p. ...
-
bioRxiv - Systems Biology 2020Quote: ... We changed medium 2 days later (Day -2) with addition of 3 μg/mL Doxycycline (Sigma D9891) to induce the NIL transcription factors ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 2 hours in a 2% bovine serum albumin solution (Sigma-Aldrich, MO, USA) and incubated overnight with primary antibodies PDGFRa (1:1000 ...
-
bioRxiv - Genetics 2020Quote: ... we added either 2 uL of EtOH (negative control) or 2 uL of EtOH plus cholesterol (Sigma), 7-dehydrocholestrol (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... The lobe was filled with 2 x 500mL of cold PBS containing 2 mM EDTA (Sigma-Aldrich). After massage ...
-
bioRxiv - Neuroscience 2023Quote: ... mice were deeply anesthetized with one injection of avertin (2,2,2-tribromoethanol 1.25%, 2-methyl-2-butanol 0.78%; 20 μl/g, intraperitoneal, Sigma-Aldrich). Mice were then perfused transcardially with 1X PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Grids were then placed in a 2% solution of poly(2-hydroxyethyl methacrylate) (Sigma-Aldrich, P3932-10G) in ethanol to create a hydrophobic coating ...
-
bioRxiv - Neuroscience 2023Quote: Mice were anaesthetized with avertin (2,2,2-tribromoethanol 1.25%; 2-methyl-2-butanol 0.78%, 20 ll/g, ip, Sigma-Aldrich) before bilateral injection of AAV on a stereotaxic frame (David Kopf Instruments) ...