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Citations for Millipore Sigma :
4801 - 4850 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated by adding 5-sulfosalicylic acid (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were blocked in 5% skim milk (Nacalai ...
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bioRxiv - Biochemistry 2023Quote: ... Activated protein was transferred to a PVDF membrane (ED Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a Polyvinylidene fluoride (PVDF) membrane (Millipore) and blocked with 5% nonfat dry milk in TBST (20 mM Tris-HCl ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... proteins were transferred onto a PVDF membrane (Merck Millipore #IPFL00010) and blocked with 5% skimmed milk-PBST ...
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bioRxiv - Microbiology 2023Quote: ... Reduced proteins were alkylated with 55 mM Iodoacetamide (Sigma-Aldrich) for 45 min at 37°C in the dark followed by the same washing-drying step described above ...
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bioRxiv - Cell Biology 2023Quote: ... protein expression was induced with 0.5 mM IPTG (Sigma-Aldrich) for 1.5 h ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred onto PVDF membrane (Immobilon-FL, Millipore) using a semi-dry transfer apparatus (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Filtered media was then loaded onto a Protein A (Millipore) column prewashed in ice cold HEPES-buffered saline (HBS ...
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bioRxiv - Physiology 2023Quote: ... Separated proteins were transferred to polyvinylidene difluoride membrane (PVDF, Millipore) using a Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a PVDF-FL membrane (Millipore Sigma) and blocked in 5% milk in 1X PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany) and the membrane was blocked with 5% fat-free milk powder in TBST and incubated for 1 h at room temperature or overnight at 4°C with primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... the proteins were visualized using a chemiluminescence detection reagent (Millipore) or directly by fluorescence (LICOR) ...
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bioRxiv - Immunology 2023Quote: Protein samples were denatured in 2x Laemmli buffer (Sigma-Aldrich) at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE ...
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bioRxiv - Cell Biology 2023Quote: ... separated proteins were transferred to ECL immobilon-P membranes (Millipore) using semi-dry transfer (BioRad) ...
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bioRxiv - Microbiology 2023Quote: The PhoP protein was expressed via pET28a(+) plasmid (Novagen, Denmark). The amplified phoP gene was inserted into pET28a using T5 recombination cloning kit ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 1.5 hours ...
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bioRxiv - Genomics 2023Quote: ... Protein concentration was determined using the Bradford assay (Sigma B6916).
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bioRxiv - Cancer Biology 2023Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich B6916). Lysates were resolved on a 4-12% Bis-Tris NuPage gel (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in 2× Laemmli buffer (Sigma-Aldrich), boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Biophysics 2023Quote: Protein samples were purchased from Sigma-Aldrich (St. Louis, MO) in lyophilized form ...
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bioRxiv - Biophysics 2023Quote: ... After transferring the proteins onto a PVDF membrane (Merck Millipore), the membranes were blocked in TBS-T (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Protein loading was determined by Ponceau Stain (Sigma Aldrich, USA). Immunoblot images were processed using Image Lab Software Version 6.1 (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The following chemicals were used: A2MG protein standard (Sigma-Aldrich), fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were diluted with Laemmli sample buffer (Sigma Aldrich) and distilled water ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg/ml protein of ECM gel (Sigma-Aldrich: E1270)) in medium without FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg/ml protein of ECM gel (Sigma-Aldrich: E1270)) in medium without FBS.
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bioRxiv - Cancer Biology 2023Quote: ... and proteins were transferred onto PVDF membranes (Immobilon-P, Millipore) that were subsequently blocked using 3% BSA ...
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bioRxiv - Immunology 2023Quote: ... proteins were immunoblotted on a PVDF-membrane (Sigma-Aldrich #3010040001) for 1h at 100V and blocked with 5% milk in TBS and 1% Tween20 ...
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bioRxiv - Developmental Biology 2023Quote: ... and proteins were then transferred to a nitrocellulose membrane (Millipore). The membranes were blocked in 5% non-fat dry milk in PBS with 0.2% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore) using a Bio-Rad transfer system at 100 V for 1 h or 25 V for 16 hrs ...
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bioRxiv - Physiology 2023Quote: ... Freshly extracted proteins were diluted in Laemmli buffer 2x (Sigma), incubated 10 min at 95°C ...
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bioRxiv - Plant Biology 2023Quote: ... and Protein A Agarose/Salmon Sperm DNA (Millipore, 16-157) were used for IP of GFP- and YFP-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... protein pellets from cardiomyocytes were dissolved in RIPA buffer (Sigma) containing 0.1% Triton X-100 and protease inhibitors (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Millipore, #IPVH0001) and blocked with 5% BSA in 0.1% Tween-20 in TBS ...
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bioRxiv - Bioengineering 2023Quote: ... The protein was purified using Ni-NTA His·Bind Column (Novagen). The purified protein was quantified using BCA protein assay kit (Pierce) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 0.1 mM IPTG for 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (IPVH00010, Immobilon, Millipore) and blocked for 1 h with Tris-buffered saline containing 0.05% Tween 20 and 3% bovine serum albumin ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (IPFL00010, Millipore, USA) and incubated with specific antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified from lysate using either nickel (Sigma-Aldrich) or glutathione resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... and whole-protein lysate was extracted using Bugbuster reagent (Novagen) and Pierce Universal Nuclease according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were then transferred to a PVDF membrane (Millipore) using a semi-dry transfer apparatus at 10V for 30 minutes at room temperature and imaged under the stain-free blot setting on Chemi-Doc to visualize the total transferred proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Total transferred protein was visualized with Ponceau S (Sigma-Aldrich) and incubated overnight in blocking buffer (Intercept Blocking Buffer ...
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bioRxiv - Microbiology 2023Quote: ... proteins were alkylated with 10 mM IAA (Sigma-Aldrich, Germany) in 100 mM TEAB at 25 °C for 20 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred onto a PVDF membrane (Millipore) using a semi-dry blotting system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein G Mag Sepharose beads (Sigma-Aldrich GE28-9513-79) were washed in cell lysis buffer and blocked in 10% BSA (Jackson ImmunoResearch 001-000-162 ...