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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The protein complex was purified via FLAG affinity chromatography (Sigma) as described59 ...
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bioRxiv - Genetics 2021Quote: ... Protein concentration was measured with Assay-free Card (EMD Millipore) and Bradford Ultra (Expedeon ...
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bioRxiv - Biophysics 2021Quote: ... the protein solution was spiked with 5μM of heparin (Sigma) and aggregation was monitored by adding 25μM of Th-T in half-area black 96-well microplates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... Goat and cow whey proteins were purchased from Sigma (USA). Heparin was purchased from Sigma (USA) ...
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bioRxiv - Immunology 2021Quote: ... Protein expression was induced by addition of L-(+)-arabinose (Sigma) to a final concentration of 0.02% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were precipitated with cold HPLC grade acetonitrile (Sigma-Aldrich). Acetonitrile was added in the ratio of 1:5 (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... The fragments were immunoprecipitated with anti-Protein A antibodies (Sigma). ChIP DNA was analyzed by both semi-quantitative PCR and quantitative real-time PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein loading was visualized using Amido black staining solution (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Millipore Sigma Immobilon-FL for near-infrared fluorescent detection ...
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bioRxiv - Microbiology 2020Quote: ... and clean-up using a PVDF protein-binding membrane (Millipore). Empower 3 software (Waters ...
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bioRxiv - Immunology 2021Quote: ... and immunoprecipitated with Sephadex protein A/G beads (Sigma Aldrich, St ...
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bioRxiv - Neuroscience 2020Quote: ... glial fibrillary acidic protein (GFAP, 1:1000, #SAB4100002, Sigma-Aldrich), NeuN (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Separated proteins were transferred onto PVDF membrane (EMD Millipore, IPVH00010) for 2.5 hours at 200 mA in cold transfer buffer (25 mM TrisHCl ...
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bioRxiv - Developmental Biology 2020Quote: ... and protein was transferred to a Immobilon-FL membrane (Millipore), which was incubated with goat anti-h/m Gli3 (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were loaded in 10 kDa centrifugal filter tubes (Millipore), washed twice with 200 μL UA buffer (8 M urea in 0.1 M Tris-HCl ...
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bioRxiv - Genomics 2021Quote: ... which were then probed with anti-Protein A antibodies (Sigma, Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin in TBST ...
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bioRxiv - Genetics 2020Quote: ... Immuno-detection of proteins was done using ECL kit (Millipore) following the manufacturer’s recommendations and were developed using G-Box iChemi XR scanner ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were alkylated with 2μl of 0.5M iodoacetamide (Sigma) for 45 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... Protein transport inhibitors Brefeldin A (10 µg/ml, Sigma-Aldrich) and Golgi Stop (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified proteins were concentrated by ultrafiltration (Amicon Ultra-4, Millipore) and diluted in PBS.
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bioRxiv - Microbiology 2022Quote: ... Proteins were subsequently transferred onto PVDF membranes (Immobilon-P, Millipore) and blocked at room temperature with either 5% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was subsequently concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated using 10K Spin Concentrator (Millipore Sigma UFC801008) and the concentration was determined using Detergent Compatible Bradford ...
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bioRxiv - Biochemistry 2022Quote: ... GST proteins were eluted with 10 mM reduced glutathione (Sigma) in 50 mM Tris pH 8.0.
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bioRxiv - Bioengineering 2022Quote: Concentrated proteins were filtered through 0.45 μm filters (Sigma #SLHV033R) and were then quantified using a Pierce BCA kit (ThermoFisher #23227 ...
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bioRxiv - Cell Biology 2022Quote: Proteins were reduced with 2.5 μl 0.2 M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were subsequently concentrated using Amicon spin concentrators (Millipore), snap-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... After the protein was transferred to a PVDF membrane (Millipore; Merck KGaA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was mixed with 2X Laemmli Buffer (Sigma-Aldrich 53401), run on BioRad 4-20% precast SDS-polyacrylamide gels ...
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bioRxiv - Cell Biology 2022Quote: ... The resolved proteins were transferred onto charged PVDF membrane (Millipore) using semi-dry blotting apparatus ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were visualized using antibodies that recognize PSTAIRE (P7962, Sigma), V5 (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μM M3814 (DNA-dependent protein kinase inhibitor) (Sigma-Aldrich) was added to the cell media for 72 hr to repress non-homologous end joining DNA repair (Riesenberg et al. ...
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bioRxiv - Neuroscience 2022Quote: ... was pre-cleared with Protein A agarose beads (P3476, Sigma). An ‘input’ sample was set aside and 900 µl of supernatant was incubated overnight with beads cross-linked to CAMK2A primary antibody (10 µg ...
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bioRxiv - Neuroscience 2022Quote: ... total tranferred protein levels were quantified using Ponceau S (Sigma) or the No-Stain Labeling Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon®-FL PVDF membrane (Millipore). Membranes were blocked in Odyssey® Blocking Buffer (LI-COR) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-microtubule associated protein-2 (MAP2) (Millipore Sigma AB5622), and mouse anti-TUBB3/β3 tubulin (Novus Biologicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The resolved proteins were electro-transferred to PVDF membranes (Millipore). The proteins of interest were detected with the specific antibodies as indicated in the figure legends and above ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100% trichloroacetic acid (Sigma 76039) to a final concentration of 20% v/v and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-Glial Fibrillary Acidic Protein GFAP (catalog #G9269; Sigma-Aldrich), anti-PP2A antibody (catalog #SAB4200266 ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were transferred to a PVDF (polyvinylidene difluoride) membrane (Millipore) using Wet transference method (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein was extracted using BugBuster Master Mix (Sigma Aldrich, 71456), mixed 1:1 with 2x laemmli sample buffer (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Protein bands were visualized with ECL-chemiluminescent kit (Millipore, MA). The Abs against LC3B was purchased from Novusbio (Littleton ...
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bioRxiv - Microbiology 2024Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Physiology 2022Quote: ... specific proteins were detected with anti-RyR2 (MA3-916, Sigma), anti-CaV1.2 (CACNA1C ...
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bioRxiv - Synthetic Biology 2022Quote: ... and lysed by 20 mL Bugbuster protein extraction buffer (Millipore, 50 mL Bugbuster lysis buffer contains - 49 mL Bugbuster reagent ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of the indicated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Proteins transferred to PVDF-FL membranes (Millipore, Billerica, MA, USA) were detected with mAb PRC5 at a dilution of 1:5000 and mAb PRC1 at a dilution of 1:2500 (23) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies against pan-talin protein (1:1000, Sigma-Aldrich) and actin control (1:2000 ...