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Citations for Millipore Sigma :
4651 - 4700 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... G-Sepharose beads (50µL for 2mg of total proteins, Sigma) were added for 1h before being washed with lysis buffer 3 times ...
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bioRxiv - Bioengineering 2019Quote: ... 20μg protein was incubated in 10% 2-Mercaptoethanol (Sigma-Aldrich) in laemmli loading buffer (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Millipore Corporation). The membranes were blocked with Blocking One (Nacalai Tesque ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations were read by a MAGPIX from EMD Millipore using xPONENT 4.2.
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bioRxiv - Biochemistry 2020Quote: ... 100 µg protein were dot blotted on PVDF membrane (Millipore), stained with Alcian blue and excised ...
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bioRxiv - Zoology 2020Quote: ... in comparison with wells containing visible protein ladder (Sigma 26616). The antibody staining intensity of each Western blot sample was normalised to its corresponding total-protein stain intensity using BioRad ImageLab software ...
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bioRxiv - Biochemistry 2019Quote: ... Solubilized proteins were applied to FLAG-M2 agarose (Sigma-Aldrich) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced with 200 pg/mL doxycyclin (Sigma). Cell cycle synchronization was performed using a combination of 2.5 mM thymidine (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Millipore, IPVH00010), nonspecific binding was blocked by incubation with 5% skim milk ...
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bioRxiv - Immunology 2020Quote: ... and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich, St ...
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bioRxiv - Biochemistry 2021Quote: ... The fusion protein was purified by Ni-NTA (Novagen, USA) affinity chromatography followed by a Superdex HiLoad 16/600 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred to polyvinylidene difluoride membrane (Millipore, MA, USA), and the membrane was blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins were transferred to Immobilon-F PVDF membranes (Millipore) at 0.8 mA/cm2 for 2 hr at RT under semi-dry blotting conditions using a discontinuous 3 buffer system (Anode Buffer I ...
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bioRxiv - Biochemistry 2020Quote: ... proteins in the gels were transferred to PVDF membranes (Millipore). Immunoblots were analyzed using primary antibodies against α4/Pre6 (D ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein extracts were made using trichloroacetic acid (TCA, Sigma) precipitation (Knop et al. ...
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bioRxiv - Microbiology 2020Quote: ... Protein was concentrated using Amicon Ultra-4 30k MWCO (Millipore). 3C protease was added to purified protein in a 1:50 (w:w ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-amyloid precursor protein (APP, clone 22C11, Millipore), mouse monoclonal anti-α-Synuclein (Syn-1 ...
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bioRxiv - Plant Biology 2020Quote: SDS-PAGE and protein gel blotting with PVDF membranes (Millipore) were performed as described (Lauber et al ...
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bioRxiv - Plant Biology 2021Quote: ... with Magna ChIP™ Protein A Magnetic Beads (Sigma-Aldrich) for Myc-NPR1 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were stained with Brilliant Blue G Colloidal Coomassie (Sigma) and the HDAC6 band of interest was excised ...
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bioRxiv - Plant Biology 2020Quote: ... and salmon sperm DNA/protein A agarose beads (Millipore, USA) were used for ChIP experiment ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to a PVDF membrane (ISEQ00010, Merck Millipore) blocked and blotted using the iBind system (SLF1020 ...
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bioRxiv - Biochemistry 2020Quote: ... Purified 5-HT2C protein immobilized on nickel agarose beads (Sigma) was incubated with a given marker ligand at 2 nM mixed with each test compound (aporphine ligands ...
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bioRxiv - Cell Biology 2019Quote: ... The resolved proteins were electro-transferred to PVDF membranes (Millipore). The proteins of interest were detected with specific polyclonal or monoclonal antibodies as indicated in the figures ...
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bioRxiv - Cell Biology 2020Quote: ... and RSV F protein (1:500 dilution, 488 conjugated; Millipore). Cultures were mounted using DAPI mounting medium (Vectashield ...
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bioRxiv - Pathology 2019Quote: ... The Protein concentration was determined using Bradford reagent (Sigma Aldrich) (Bradford et al. ...
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bioRxiv - Genetics 2021Quote: ... ChIP-grade protein A+G magnetic beads (Millipore, 16-663) were added and the enriched chromatin was eluted with 150 uL ChIP Elution Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was then induced with 50 μM copper (Sigma). After 3 hrs of induction ...
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bioRxiv - Cell Biology 2020Quote: The total protein concentration was determined by Bradford Assay (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich) and 30 μg of protein per sample was electrophoresed by SDS PAGE on 4–20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred onto a PVDF membrane (0.45μm, IPVH00010, Millipore) and detected using antibodies targeting p16INK4A (Abcam ...
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bioRxiv - Physiology 2021Quote: ... followed by peroxidase-conjugated Protein G (Sigma P8170; 1:130’000) or peroxidase-conjugated anti-mouse IgG (Fab Specific ...
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bioRxiv - Biochemistry 2021Quote: ... The column was calibrated using the following protein standards (Sigma); ferritin (12 nm – 440 KDa) ...
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase conjugated secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... proteins were transferred onto a polyvinylidene difluoride membrane (Immobilon, Sigma). The membrane was blocked using skimmed milk and treated with the primary antibody for overnight ...
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Enzymes degraded under high light maintain proteostasis by transcriptional regulation in ArabidopsisbioRxiv - Plant Biology 2021Quote: ... A total plant protein extraction kit (PE0230-1KT, Sigma Chemicals) was used to extract total proteins ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were incubated with anti-FLAG M2 resin (Sigma) for overnight with nutation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were transferred onto a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2021Quote: ... after which Protein G agarose/Salmon sperm beads (Merck Millipore) were added and incubated for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression was induced by 2 μg/mL doxycycline (SIGMA) for 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra, Sigma part no. 5892791001), 1mM PMSF.
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were reduced with tris-2-carboxyethyl phosphine (ΤCEP, Sigma) for 1h at 60°C at a final concentration of 5mM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (IPFL00010, Merck Millipore) and blocked with 5% skimmed milk- PBST ...
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bioRxiv - Cancer Biology 2020Quote: Immunoprecipitation was performed using an Immunoprecipitation kit (Protein G, Sigma), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Magna ChIP ™ Protein A/G conjugated magnetic beads (Millipore) were used to immunoprecipitate antibody-bound chromatin complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were detected using a chemiluminescent detection kit (Millipore) and the Fuji Super RX-N X-ray films or an Amersham Imager 680.
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were developed with HRP-Substrate ECL (Millipore) detected with the Alliance Q9 system.
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bioRxiv - Cancer Biology 2021Quote: ... Total protein content was determined by Precision Red Reagent (Sigma) protein assay ...
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bioRxiv - Biophysics 2020Quote: ... The protein was concentrated using a centrifugal filter (Merck Millipore) and the concentration was determined by UV absorbance using the corresponding ε values (M-1 cm-1).
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualized by ECL detection system (Millipore) or ODYSSEY (LI-COR).