Labshake search
Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: mESC protein samples were isolated from mESC pellets using ice-cold RIPA buffer (Sigma) in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen) ...
-
bioRxiv - Cell Biology 2024Quote: ... to stain lectin and nuclei were labeled with DAPI (1:2000, Sigma). Cells were imaged at 120X magnification using either an inverted fluorescent microscope (Olympus ...
-
bioRxiv - Cell Biology 2024Quote: ... protease inhibitor cocktail (1:1000; Sigma-Aldrich) and Phosphatase Inhibitor Cocktail 3 (1:100 ...
-
bioRxiv - Cell Biology 2024Quote: ... and Phosphatase Inhibitor Cocktail 3 (1:100; Sigma-Aldrich). Lysates were clarified by centrifugation at 14,000 x g for 1h at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoreses (SDS-PAGE) on 15% homemade gel and transferred to Immobilon-P transfer membranes (Millipore Corp).
-
bioRxiv - Cell Biology 2024Quote: ... blocked with PBS containing 2% bovine serum albumin (BSA; Sigma-Aldrich) for 45 min and subsequently incubated with primary and secondary antibodies for 1 h ...
-
bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
-
bioRxiv - Cell Biology 2024Quote: Doxycycline (D1822; Sigma) 1 μg/ml.
-
bioRxiv - Molecular Biology 2024Quote: ... mice were fed with doxycycline (Sigma-Aldrich, 200µg/mL in 5% sucrose) for the indicated time ...
-
bioRxiv - Molecular Biology 2024Quote: ... The signal was detected using Immobilon Western chemiluminescent HRP substrate (Merck Millipore). Blot images were captured using the Fusion Solo S system (Vilber Lourmat ...
-
bioRxiv - Microbiology 2024Quote: ... flasks were coated with 2-5ml of pre-warmed 1% gelatin (Sigma Aldrich) dissolved in PBS and incubated at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... Primary detection was performed using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000), anti-V5 mouse monoclonal antibody (Cat# V8012 ...
-
bioRxiv - Microbiology 2024Quote: The 293T cells were seeded in a T75 cm2 flask (1 x 106) and the next day were transfected with plasmid DNA using GeneJuice transfection reagent (EMD Millipore) as per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... rabbit polyclonal anti-RBM25 (Sigma, HPA070713, 1:200), rabbit polyclonal anti-RBM14 (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... rabbit polyclonal anti-RBM14 (Sigma, HPA006628, 1:50), rabbit monoclonal anti-TAF1 (in-house/Abcam ...
-
bioRxiv - Molecular Biology 2024Quote: ... The filtrate was incubated for 2 hours with 200 µL anti-FLAG M2 affinity resin (Sigma) that was prewashed with lysis buffer.
-
bioRxiv - Cell Biology 2024Quote: ... MISSION esiRNA (Sigma-Aldrich, USA, EHU081051-20UG) was used for depletion of the human SHIP2 ...
-
Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... anti-CDHR5 (Rabbit, Sigma #HPA009173), 1:250 ...
-
Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... anti-ZO-1 clone R40.76 in CL4 (rat, EMD Millipore Sigma #MABT11), 1:100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Sections were de-paraffinized and rehydrated through xylene substitute (Sigma-Aldrich) and graded ethanol solutions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Anti-FLAG M2 magnetic beads (Sigma-Aldrich) or protein G dynabeads (Invitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... Rabbit anti-MUC5B (Sigma-Aldrich; HPA008246). DAPI (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mouse anti-ACT (Sigma-Aldrich; T6793). Rabbit anti-MUC5B (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... DAPI (Sigma-Aldrich; D9542). AF488 Donkey anti-rabbit (Life Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... The buffer was filtered using a 0.22-μm disposable membrane cartridge (Millipore). The fractions were eluted offline over a 60min gradient through a dual column set-up of a YarraTM 3 μm SEC-4000 (300 x 7.8 mm ...
-
bioRxiv - Molecular Biology 2024Quote: ... was diluted 1:8000 and Rabbit anti-MUC5B (Sigma-Aldrich; HPA008246) was diluted 1:500 in staining buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... Nuclear extract was incubated with M2 anti-FLAG affinity gel (Sigma A2220) for 2 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with Benzonase (Sigma-Aldrich, E1014-25KU) and Halt™ Protease/Phosphatase Inhibitor Cocktail (ThermoFisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... and DAPI (Sigma-Aldrich; D9542) diluted 1:1000 in staining buffer were added to the ALI transwells and incubated for 1 hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10µM CFTRinh-172 (Sigma Millipore, #C2992) apically to inhibit CFTR channel ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mouse anti-ACT (Sigma-Aldrich; T6793) was diluted 1:8000 and Rabbit anti-MUC5B (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10µM CFTRinh-172 (Sigma Millipore, #C2992) apically to inhibit CFTR channel ...
-
Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... BMMs were transduced with the viral supernatant along with 10 μg/mL of protamine sulfate (Sigma-Aldrich) for 24 h ...
-
bioRxiv - Cell Biology 2024Quote: ... using Polyethyleneimine (Sigma-Aldrich, USA, #408727). DNA was added in a ratio of 4:2:1:1 ...
-
Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: The lentiviral constructs containing PLCβ4 small hairpin RNA (shRNA) or a non-specific shRNA control (Con-sh) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Lentiviral particles were generated by transfecting 293T cells with the expression constructs ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 ml of 2 µM LatrunculinA (Sigma Aldrich) was spiked-in to the imaging dish at the 4 min mark to bring the final concentration of LatrunculinA to 1 µM ...
-
bioRxiv - Cell Biology 2024Quote: ... and HeLa wild-type and Drp1 KO cells (kindly provided by Mariusz Karbowski; (Oshima et al., 2021)) were cultured in DMEM (Sigma-Aldrich; D5796) that was supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... that was supplemented with 10% fetal bovine serum (Sigma-Aldrich; F0926), 25mM HEPES ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1% penicillin/streptomycin (Sigma-Aldrich; P4333). All experiments using CRISPRi cells were performed on early passages (<10 ...
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM d-Desthiobiotin (Sigma-Aldrich)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mg/ml DNase (Sigma, Cat# DN25) for 1-1.5 hours at 37 ℃ with rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... containing 0.25% collagenase (Sigma, Cat# C9091), 0.01 M HEPES (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... 24h post-treatment cells were washed using room temperature PBS 1X and transferred to an Attofluor Cell Chamber (Sigma Aldrich, A7816), 5 μM of DHE was added in HBBS medium (Hanks’ Balanced Salt Solution ...
-
bioRxiv - Cell Biology 2024Quote: ... for 5 min or 20 µg/mL anti-β-tubulin antibodies (in PBS, #T7816, Sigma) for 5 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 1x protease inhibitors (#04693159001, Sigma) and 0.05% Triton X-100 (# X100 ...
-
bioRxiv - Cell Biology 2024Quote: ... chambers were assembled by melting thin strips of parafilm in between two glass coverslips silanized with 0.05% dichlorodimethylsilane (DDS, #440272, Sigma). The chambers were incubated with 20 µg/mL anti-biotin antibodies (in PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... were diluted into BRB80T (BRB80: 80mM PIPES pH 6.9, 1mM EGTA, 1mM MgCl2, supplemented with 10 µM paclitaxel (#17191, Sigma)) ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by 1% Pluronic (F127 in PBS, #P2443, Sigma) for at least 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell pellets were resuspended in 0.5 pellet volumes of lysis buffer (BRB80 supplemented with 1x phosphatase inhibitors (#4906845001, Sigma), 1x protease inhibitors (#04693159001 ...
-
bioRxiv - Cell Biology 2024Quote: ... The chambers were incubated with 20 µg/mL anti-biotin antibodies (in PBS, #B3640, Sigma) for 5 min or 20 µg/mL anti-β-tubulin antibodies (in PBS ...