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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitors (Sigma-Aldrich), kept on ice for 10 min and centrifuged at 16,000 x g for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The inhibitor was reconstituted in 10% DMSO (Sigma Aldrich), 40% PEG300 (MedChemExpress) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-laminin and anti-MHC-I antibodies were purchased from SIGMA. Anti-MHC-IIA was a kind gift of Dr Schiaffino (Department of Biomedical Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... washed three times with 0.1% BSA/PBS before they were incubated with DAPI (Sigma-Aldrich) in 200 μL for 5-10 min on ice and flow sorted on an ARIA I or ARIA III BD sorters using a 70 μm nozzle ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial membrane potential was evaluated using the potentiometric dye tetramethyl rhodamine methyl ester (TMRM; Sigma Aldrich) at a final concentration of 10 nm ...
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bioRxiv - Cell Biology 2024Quote: ... fluorophore-conjugated secondary antibodies were incubated at room temperature for 30-60 minutes in blocking buffer together with DAPI (Sigma-Adrich,1μg/ml). All washes were performed in Tween/PBS (0.2%) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were co-stained with Rhodamine-phalloidin (1:250; Sigma Aldrich #P1951), and DAPI (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG; Sigma-Aldrich, A6154). Protein expression was detected using ImageSaver6 software via an EZ capture MG system (ATTO).
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bioRxiv - Cell Biology 2024Quote: ... Sample values were referred to a p-nitrophenol (Sigma-Aldrich) standard curve and calculated as ng/mL/min.
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated by incubating wing discs in 10x trypsin-EDTA solution (#T4174, Sigma-Aldrich,) in the presence of the DNA dye Hoechst 33342 (#10337G ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies used were as follows: anti-Flag from mouse (#F1804, Sigma-Aldrich, 1:2000), anti-Lamin Dm0 from mouse (Developmental Studies Hybridoma Bank ADL84.12 deposited by P ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-phospho-Histone H3 (pHH3) from rabbit (EMD Millipore, 1:200), anti-Histone pH2AvD from rabbit (#601-401-914S ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies used for WB were as follows: anti-α-Tubulin DM1A from mouse (#T6199, Sigma, 1:5000), anti-Lamin Dm0 from mouse (Developmental Studies Hybridoma Bank ADL84.12 deposited by P ...
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bioRxiv - Cell Biology 2024Quote: ... 0.35mM proline (all Sigma-Aldrich, Darmstadt, Germany), 1% ITS™+ Premix (Corning Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilization was carried out by treating the samples with 0.1% Triton X-100 (T8787, Sigma-Aldrich) during 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... stained with Safranin O (0.2% w/v in 1% acetic acid, Sigma-Aldrich) for proteoglycan detection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T used for lentiviral transduction were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich, UK) supplemented with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Cell Biology 2024Quote: ... RPE-1 Cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F-12; Sigma-Aldrich, UK) media containing 0.365 mg/ml L-glutamine (Lglut) ...
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bioRxiv - Cell Biology 2024Quote: ... Doxycycline was purchased from Sigma-Aldrich, UK and used at 1 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... + 1% [w/v] BSA (Sigma A2153). Cells were incubated with primary antibodies overnight at 4°C in PBST + 1% [w/v] BSA ...
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bioRxiv - Cell Biology 2024Quote: ... + 1 mg/mL tRNA (Sigma 10109541001), and used to stain cells at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... All samples were washed and stored in PBS containing 2% FBS (Sigma-Aldrich) and 5 mM of EDTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2024Quote: ... in which the 2x SSC buffer (300 mM NaCl, 30 mM sodium citrate, pH = 7.0) was diluted from 20x SSC (Sigma S6639) using UltraPure DNase/RNase-Free Distilled Water (Thermo 10977015) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS and lysed in RIPA lysis buffer (Sigma-Aldrich) supplemented with complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Stable expressors were derived by selection with 5 μg/mL puromycin (Sigma-Aldrich, UK). Doxycycline was purchased from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells cultured in each 35 mm dish were dissolved in 200 – 300 μL RIPA buffer (Thermo 89900) + 2 mM PMSF (Sigma P7626) + 3 μL Protease Inhibitor Cocktail (PIC ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 10 min at RT with 0.1% [w/v] sodium borohydride (NaBH4, Sigma 452882) in PBS to reduce GA ...
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bioRxiv - Cell Biology 2024Quote: ... which consists of SSC wash buffer + 10% [w/v] dextran sulfate (Sigma D8906) + 2 mM vanadyl-ribonucleoside complex (NEB S1402 ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 mM of EDTA (Sigma-Aldrich) before acquisition ...
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bioRxiv - Cell Biology 2024Quote: ... + 2.5 mM MgCl2 (Sigma 5985-OP) with occasional pipette aspiration ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% Dimethylformamide (Sigma) and 0.6 mg ml-1 Fast Red Salt (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 6.3 ng ml-1 progesterone (Sigma), 5.2 ng ml-1 sodium selenite (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated with the “staining solution” which contained 0.1 mg ml-1 Naphtol phosphate (Sigma), 0.5% Dimethylformamide (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were treated with 0.1% of gelatin (Sigma) at 37°C for at least 20 min and then mitomyzed SNLs were plated at high density (2.5×106 cell/plate ...
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bioRxiv - Cell Biology 2024Quote: ... retrovirus mixture was supplemented with 4 µg ml-1 of Polybrene (Sigma). NSCs were then incubated for 14-18 hours at 37°C in a humidified incubator ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred by wet blot transfer on 0.2 µM pore size PVDF membrane (Amersham, GE10600021) and immunoblotted using MCU (Sigma-Aldrich, HPA016480) according to the standard protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was analyzed by WST-1 (Sigma, 11644807001) at 450 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... and shATF3#3 (TCRN000013568) were purchased from Sigma Aldrich (St. Louis, MO, USA). shControl (1864) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μg/ml Insulin (Sigma-Aldrich, St. Louis, MO, cat#: 10516), and 1% Penicillin/Streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... proteosome loading solution was prepared according to a fluorometric kit (Sigma Aldrich, MAK172) and added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... 150,000 cells were plated onto glass coverslips (for immunofluorescence) or into 12-well culture dishes coated with 10 μg/ml fibronectin (Sigma-Aldrich, F1141) diluted in DPBS with Mg2+ and Ca2+ ...
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bioRxiv - Cancer Biology 2024Quote: ... Monoclonal Anti-FLAG M2 and Monoclonal Anti-β-actin were from Sigma-Aldrich. Phospho-GATA2 (Ser192 ...
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bioRxiv - Cancer Biology 2024Quote: ... from Cell Signaling Technologies and anti-Myc-Cy3 (Sigma). Recombinant hEREG (1195EP025CF ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μM GSK3 inhibitor (CHIR99021, Sigma-Aldrich), and 10 μM ROCK inhibitor (Y27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with media containing 3% dextran (Leuconostoc spp., Mr 450,000–650,000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Vinculin (Cat# MAB3574, Millipore), rabbit anti-p27 Kip1 phospho-Serine 10 (Cat# ab62364 ...
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bioRxiv - Cancer Biology 2024Quote: ... Doxorubicin (Sigma Aldrich), EMD534085 (Medchem Express) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μM ALK inhibitor (A83-01, Sigma-Aldrich, Saint Louis, MO, USA), 5 μM GSK3 inhibitor (CHIR99021 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed using Laemmli buffer (Sigma) supplied with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) ...