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Citations for Millipore Sigma :
4551 - 4600 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... The desalted peptide solution was analyzed by LC-MS/MS using a C18 ZipTip® (Millipore, Burlington, MA, Cat. No. ZTC18S096).
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bioRxiv - Biochemistry 2023Quote: ... before incubation in 60 µL 3xFLAG elution solution (146 ng/µL of 3xFLAG peptide [Sigma-Aldrich, F4799] in 0.97X equilibration buffer) at 25°C with shaking at 1,100 rpm for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in TBS.
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in Native Elution Buffer.
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bioRxiv - Biochemistry 2024Quote: ... and Cdc13 was eluted by incubating the resin with 2 mL of K buffer supplemented with 200 µg/mL of 3x FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using Amicon Ultra 0.5 mL 100 k MW cutoff centrifuge filters (Millipore) ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide clean-up was performed on mixed-phase columns (PreOmics®) according to the manufacturer’s protocol using triethylamine (TEA, Sigma-Aldrich) instead of NH4OH in the elution buffer due to subsequent TMT labelling ...
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bioRxiv - Immunology 2020Quote: ... and product intensity measured by Software ImageJ(83) and normalized to ACTIN amplicon products (5’-GACGACATGGAGAAAATCTG-3’ and 5’-ATGATCTGGGTCATCTTCTC-3’, Sigma-Aldrich, St. Louis, Missouri, USA).
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bioRxiv - Neuroscience 2021Quote: ... FW 5’ – CCA CAT GGG AGA GTC ACA T −3’ and RV 5’-ATA GCC TGG AAG CGG TCA GAT G −3’ (Sigma-Aldrich Japan, Inc., Tokyo, Japan). The PCR products (521 bp ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Neuroscience 2022Quote: ... BrdU injections were done 24h before sacrifice for the analysis at 3 dpKA and 3/7 days post administration of zinc (Cat#83265-250ML-F, Sigma-Aldrich, St Louis, MO, USA). To identify newborn cells at 14 dpKA ...
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bioRxiv - Plant Biology 2024Quote: ... 2-(1H-Indol-3-yl)-4-oxo-4-phenyl-butyric acid (PEO-IAA; OlChemIm, Olomouc, Czech Republic) and indole-3-carbinol (I3C; Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The sections were then dehydrated in ascending concentrations of ethanol for 5 min each (2[×[35%, 50%, 70%, 80%, 90%, 3[×[100%) and washed 3 times for 5 min with propylene oxide (Sigma-Aldrich, #cat 110205-18L-C). Next ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were permeabilized for 1 min with 0.1% Triton X-100 in PBS and saturated with blocking buffer (1% BSA in PBS) before incubation for one hour with anti-α-Tubulin (T5168. SIGMA; 1:2.000) in PBS containing 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Monolayers were permeabilized with 0.5% Triton X-100 and blocked with a blocking buffer containing 10% goat serum (cat# G9023, Sigma-Aldrich, St. Louis, MO) and 1% bovine serum albumin (cat# A3803 ...
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bioRxiv - Neuroscience 2020Quote: ... and treated with blocking solution containing 1 % bovine serum albumin (Fisher Bioreagents, Cat.# BP1605-100) and 10 % normal goat serum (Sigma-Aldrich, Cat.# G9023) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slots were washed with one volume of DPBS before washing the whole membrane with TBS and blocking with 5% skimmed milk powder (Sigma-Aldrich, Munich, Germany) in TBS for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked in 1:1 mixture of 1XPBS : Intercept PBS blocking buffer (LiCor) and then incubated with anti-PAP (Sigma, P1291, lot 92557) or anti-MYC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... The wells were subsequently blocked for 1 hr in blocking solution (25 µL of 1% BSA [Sigma], 0.2% Tween-20 in 1x PBS) then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455® ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2019Quote: ... Free-floating slices were washed for 3 × 10 min periods in PBS before blocking and permeabilization for 2 h with 0.3 % Triton X-100 in PBS and 4% normal goat serum (Sigma-Aldrich, Oakville, Ontario, Canada). Slices were incubated with streptavidin conjugated with Alexa Fluor 555 or 546 (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2021Quote: ... and rehydrated for 20 mins with 1X PBS, followed by blocking buffer containing permeabilization solution (Myers et al., 2014) with either 5% normal donkey (EMD Millipore, S30-100ml) or goat serum (Thermofisher scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... in PBS (blocking buffer) was used to block the cells for one hour and replaced with anti-FLAG antibodies (Sigma Aldrich, F-1804) diluted in blocking buffer (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... fluorophore-conjugated secondary antibodies were incubated at room temperature for 30-60 minutes in blocking buffer together with DAPI (Sigma-Adrich,1μg/ml). All washes were performed in Tween/PBS (0.2%) ...
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bioRxiv - Microbiology 2024Quote: ... Thereafter the cells were incubated at 4 °C overnight in blocking-solution containing the primary antibody (Rabbit anti-hemoglobin (SIGMA, Cat. No. H4890) diluted 1/1000) ...
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bioRxiv - Neuroscience 2023Quote: ... in a staining jar and incubated for 2 h at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item# G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 72-hours in the same blocking solution with rabbit anti-CRF (Peninsula,T-4037, 1:1K) and mouse anti-TH (Millipore, MAB318, 1:5K) at 4°C on a shaker followed by 24-hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were permeabilized and blocked for an hour at room temperature using blocking buffer made fresh with the following ingredients: PBS containing 10% donkey serum (Sigma-Aldrich, catalog #D9663), 10% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were then blocked for 30 min at room temperature in blocking buffer containing 1% (wt/vol) BSA (Sigma-Aldrich, A9647-100G), 22.52 mg/mL glycine (AmericanBio ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G, Sigma-Aldrich] in TBST) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich in PBS) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4°C with primary antibody in blocking solution : anti-vinculin (Sigma-Aldrich, catalogue no. V9131, dilution 1:400) followed by three washes with PBS Tween 0.2% ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML]) for 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed with PBS (3x10 min at room temperature) and incubated for 2 hours at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item # G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with blocking buffer (PBST containing 10% normal goat serum [Thermo Fisher Scientific, 16-210-064] or donkey serum [Sigma-Aldrich, D9663]) at room temperature for 1 hr ...
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bioRxiv - Genetics 2023Quote: ... then blocking solution was added to each well consisting of PBS containing 0.5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA). Cellswere incubated in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Neuroscience 2024Quote: ... floating sections were washed in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in a blocking buffer of 10% normal donkey serum (Millipore, cat# S30-100ML) and 1% Triton X-100 in TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743; anti-pan-cytokeratin, Millipore Sigma, C5992 ...