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Citations for Millipore Sigma :
4501 - 4550 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 µL/well of 1 ng/µL solution of fragments 308-331 or 421-438 from HIV gp120 peptides or fragment 579-601 of HIV gp41 (Sigma-Aldrich) in carbonate-bicarbonate buffer (pH 9.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Elution of the co-immunoprecipitated complex was performed by gentle shake of the beads at 4°C in elution buffer supplemented with 3X FLAG peptide (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and eluted with FLAG elution buffer [identical to FLAG wash buffer but also containing 3x FLAG peptide (100 µg/ml, Sigma Aldrich)] ...
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bioRxiv - Bioengineering 2020Quote: ... Bound proteins were eluted by competition with four 2 x CV of DPBS containing 100 µg/mL FLAG peptide (Sigma Aldrich). Purified proteins were formulated in DPBS by buffer exchange through desalting columns (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were prepared in PBS and contained either 0.2 μM of the respective DegP enzyme (monomer concentration) and 7.5 μM of p23 peptide or 10 μM of the enzyme with 50 μM β-Casein (Sigma-Aldrich) and 100 μM of the Abz-KASPVSLGYNO2D reporter peptide ...
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bioRxiv - Neuroscience 2021Quote: ... Arc-Myc-FLAG was eluted by incubating beads in 50 µL of RIPA buffer containing 25 µg of FLAG peptide (Sigma-Aldrich) for 2 h at 25°C with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... subtilis was achieved by 5 min incubation with small cation specific channel-forming antimicrobial peptide Gramicidin ABC (10 µM; gABC; Sigma Aldrich), and membrane permeabilisation by 5 min incubation with pore-forming lantibiotic Nisin (10 μM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with increasing concentrations (0 to 120 μg/ml) of a reverse sequence control peptide (Ser-Asp-Gly-Arg-Gly/SDGRG, Sigma-Aldrich) and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3xFLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... in-house identifier: AfR364-3P2) and hCAP- D3 pS1474 peptide (C-QQWNVRpSPARNK; in-house identifier: AfR358-3P2) were generated commercially (SIGMA Genosys). Secondary antibodies used in the current study were as follows ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6J mice were injected subcutaneously in the flanks with 100 μg of MOG35-55 peptide (Polypeptide) emulsified in 100 μl of CFA (Sigma-Aldrich) supplemented with 50 μg of heat-killed Mycobacterium tuberculosis H37Ra (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were reduced with 4 mM DTT for 30 min at 25°C and alkylated by 10 mM iodoacetamide (Sigma-Aldrich) for 45 min in dark in a thermoshaker ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 500 μg Flag-GST-T6B peptide [46] and 80 μl of anti-Flag M2 Magnetic beads (Sigma, #M8823) for at least 3 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and sDCs were stimulated for 18 h with 1µg/ml of SIINFEKL peptide (OVA 257-264, Proimmune) or with 1mg/ml of low endotoxin soluble ovalbumin protein (sOVA; Sigma-Aldrich) in combination with NaCl or 5 ng/ml of IFNα ...
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bioRxiv - Cell Biology 2022Quote: ... 2% glycerol) and then eluting by shaking with 15 μL of 100 μg/μL of 3X FLAG peptide (Sigma-Aldrich, F4799) in the original lysis buffer for 30 min at 4°C.
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... the digested tryptic peptides from the pull-down samples and from the control group were desalted using C18 ziptips (EMD Millipore). Samples were further reconstituted in liquid chromatography-mass spectrometry (LC-MS ...
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bioRxiv - Bioengineering 2019Quote: ... a machined 48-vial rack holding 5 mL fritted peptide synthesis vessels (Torviq SF-0500) with 500 µL dimethylformamide (DMF; Sigma-Aldrich) in each was placed on the deck’s plate slot ...
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bioRxiv - Genetics 2020Quote: ... Protein-DNA complexes were eluted from the beads by adding 200 μL of ice-cold M2 buffer without detergent containing 3xFLAG peptides (Sigma-Aldrich) (final concentration of 150 ng/μL ...
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bioRxiv - Cancer Biology 2021Quote: ... and Ago1-4 were pulled down using 500 μg of Flag-GST-T6B peptide (50) and with 80 μl anti-Flag M2 Magnetic beads (Sigma #M8823) for 3 hrs on a rotor at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 5 times with RNase-free lysis buffer followed by elution with 50 µl RNase-free lysis buffer supplemented with 100 µg/ml 3xFLAG peptide (Sigma #F4799) for 30 min at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2019Quote: ... Analysis by Western blot shows a band at 19 kDA from rat olfactory bulb which is blocked by inhibition with specific OMP immunizing peptide (Sigma datasheet).
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Biochemistry 2021Quote: ... PAR chains from a purified tetra-ADP-ribosylated H2B (1-16) peptide were subjected to treatment with Alkaline Phosphatase (Sigma-Aldrich) and Phosphodiesterase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... FITC-labelled peptide samples (Li, i+4L, Li, i+4D or the RCM control) were dissolved in 100% rat serum (Sigma Aldrich) at an initial concentration of 4 μM ...
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bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the specificity of the CRF antibody in these tissues was demonstrated by preadsorption with 10X and 100X CRF peptide (Sigma-Aldrich), both of which eliminated CRF immunoreactivity [15].
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bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The emulsion was prepared by mixing MOG35-55 peptide (United Biosystems, Cat# U104628) and complete Freund’s adjuvant (Sigma-Aldrich, Cat# F5881) with additional Mtb (BD Difco ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated with 1 µg/ml of gp33 peptide in the presence of 10 µg/ml Brefeldin A (Sigma-Aldrich) at 37°C for 6 h ...
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bioRxiv - Immunology 2022Quote: ... The proteins captured on the anti-FLAG resin were eluted by incubation with 46 μl of TBS containing 150 ng/µL of 3X FLAG peptide (Sigma, #F4799). For mass spectrometry (MS ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The desalted peptides in the cartridge were eluted using a buffer containing 0.5% acetic acid and 80% acetonitrile (Sigma-Aldrich-Aldrich). The samples were subsequently dried using a speedvac for mass spectrometry analysis.
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of biotinylated peptides were bound to 25-50 μl of a 50:50 slurry of streptavidin agarose (Sigma-Aldrich), mixed with recombinant proteins or cell lysates ...
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bioRxiv - Neuroscience 2022Quote: Seven-week isogenic control- and healthy control hiPSC derived neurons were treated with 2.5 μM Aβ peptide 1-42 (Sigma, PP69-0.25MG) for 24 hours.
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Immunology 2023Quote: ... injected into the left and right flank with 100 μl of an emulsion of MOG35-55 peptide (500 μg/ml) (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (made from incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... the affinity columns were eluted with 500ul of 0.5ug/ul FLAG peptide prepared according to the manufacturer instruction (Sigma-Aldrich #F3290).
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bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with 10nM SIINFEKL (OVA) peptide for 5h at 37°C in the presence of Brefeldin A (Sigma–Aldrich) for the last 2h before staining ...