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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Frozen stocks of macrophage THP-1 cells (ATCC TIB-202) were thawed in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were stably differentiated into macrophages by phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) at 5 ng/ml over 48h ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Immunology 2020Quote: 5 × 105 macrophages were fixed in 2.5% glutaraldehyde (Sigma) in 100 mM sodium cocodylate (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Macrophages were then harvested by incubation with Accutase (Sigma) at 37 °C to gently detach cells followed by centrifugation at 300 x g for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Macrophages were plated on poly-L-lysine (Sigma-Aldrich)-coated coverslips ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were plated on poly-L-lysine (Sigma-Aldrich)-coated coverslips ...
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bioRxiv - Molecular Biology 2023Quote: Macrophages were lifted from plates using Accutase (Sigma-Aldrich) and immediately incubated with 1:1000 Zombie-NIR (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... macrophages were harvested after incubation with Accutase (Sigma-Aldrich) for 15 minutes along with gentle cell scraping.
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Neuroscience 2023Quote: Western blot analysis of MBP was performed on protein lysates from the anterior cerebrum at P22 as previously described [32] using rat anti-MBP (Millipore MAB386 1:500) and anti-β-actin (Sigma-Aldrich AC-74 ...
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bioRxiv - Immunology 2021Quote: Transiently expressed antibodies were purified from 30 ml of HEK 293 Expi cultures using Protein G GraviTrap columns (Sigma, GE28-9852-55) and the Ab Buffer Kit (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10,000 in ELISA buffer (PBS, 5% Tween20, 1% goat serum (Sigma-Aldrich, G9023)) was added to all wells ...
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bioRxiv - Neuroscience 2019Quote: ... calcitonin gene-related peptide (CGRP, mouse; 1:100, Sigma, USA), vasoactive intestinal peptide (VIP ...
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bioRxiv - Microbiology 2023Quote: ... both genes were inserted into the pETDueT-1 plasmid (Novagen) in two steps ...
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bioRxiv - Physiology 2022Quote: ... or ELISA (ghrelin; Millipore).
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bioRxiv - Neuroscience 2021Quote: ... GFAP was also measured in the supernatant with a sandwich ELISA kits (Sigma NS830) using a 200x dilution ...
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bioRxiv - Physiology 2019Quote: ... P10 and 10 weeks of age using a commercially available leptin ELISA kit (Millipore). Serum free fatty acid levels were assayed in chow-fed or HFHS-fed mothers at gestational day 16 and in the offspring of chow- or HFHS-fed dams at P10 and 10 weeks of age using a commercially available FFA kit (Abcam).
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bioRxiv - Microbiology 2023Quote: ... and IL-18 (BMS672TEN) in supernatants were measured using the ELISA Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neuropeptide Y levels were measured using commercially available ELISA kits (EMD Millipore, EZHNPY-25K).
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... A primary antibody (rat anti-HA, 1:1,000, Sigma, 3F10) was diluted in 5% fetal bovine serum/TBST and the membrane was incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... rat monoclonal anti-Nicotinic Acetylcholine Receptor (Sigma-Aldrich, 1:800). The following secondary antibodies (diluted in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Flag rat (Sigma Cat# SAB4200071; 1:500 surface ICC), anti-Myc rat (Abcam Cat# ab206486 ...
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bioRxiv - Cell Biology 2021Quote: ... rat antibody against tyrosinated tubulin (1:100; Sigma Aldrich, MAB1864), and mouse antibody against detyrosinated tubulin (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... rat monoclonal anti-HA (1:800, clone 3F10 Roche/Sigma).
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bioRxiv - Microbiology 2020Quote: ... and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma) for MLKL detection followed by treatment with 1:1000 chicken anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma) for MLKL detection followed by treatment with 1:1000 chicken anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... rat anti-HA clone 3F10 (diluted 1:3000, Sigma #2158167001), mouse anti-GAPDH clone 6C5 (diluted 1:3000 ...
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bioRxiv - Plant Biology 2021Quote: ... and rabbit anti-rat-peroxidase A9542 (1:20000, Sigma-Aldrich) with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... rat anti-HA (1:1000, Millipore Sigma cat#11867423001, RRID:AB_390918), goat anti-chicken Alexa 488 (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... the primary antibody used was 1:100 ChAT (rat, Millipore), and the secondary antibody was 1:250 anti-goat-Cy3 (Jackson Immunoresearch) ...
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bioRxiv - Neuroscience 2020Quote: ... and goat anti-rat Cy3 (1:500, Millipore, Burlington, MA) for 4-h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Blocking component 2 (B2): 1 mg/ml rat IgG (Sigma) in S2.
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bioRxiv - Plant Biology 2019Quote: ... or goat α-rat IgG-HRP (1:10000, Sigma-Aldrich) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... rat monoclonal antibody (mAb) anti-HA (3F10, 1:500, Sigma), mouse mAb anti-Arm (N2-7A1 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA (1:100, clone 3F10, Sigma-Aldrich, #11867423001), mouse anti-ubiquitin conjugates (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... rat anti-Ser2 Pol II (AB_11212363, Merck Millipore, 1:5000). Respective secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... and donkey anti-rat Cy3 (0.002 μg.μL-1, Millipore AP189C). After rinsing three times with PGT 0.125% buffer and once with PB ...
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bioRxiv - Immunology 2022Quote: ... 1:1000 rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Rat insulinoma cell line INS-1 832/3 (Millipore Sigma) was cultured in RPMI-1640 supplemented with 2mM L-Glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... and rat anti-human E-cadherin (EMD Millipore, DECMA-1) at a concentration of 1/200 in a humidified incubator overnight ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-substance P (1:400; Millipore Cat# MAB356, RRID:AB_94639), or rabbit anti-DsRed (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... a rat anti-DAT (1:2000, Millipore Cat# MAB369, RRID:AB_2190413), a rabbit anti-VMAT2 (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-ZO-1 clone R40.76 (rat, EMD Millipore Sigma #MABT11), 1:100 ...
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bioRxiv - Genetics 2023Quote: ... Rat anti-RNA Pol II (#04-1571 Millipore, 1:1000), rabbit anti-pH3 (#SC-8656-R Santa Cruz Biotechnology 1:100) ...